The influence of short-chain fatty acids, vitamins and some other factors on the sulphate-reduction in lactate cultures ofDesulphovibrio desulphuricans (Beijerinck), Kluyver and van Niel

Ghose, T. K.

doi:10.1007/bf02483577

Cited by 3 publications

(4 citation statements)

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“…Estimation of sulphide in the gas phase was made by the passage of the gas produced during fermentation through alkaline cadmium hydroxide solution prepared by dissolving 4.3 g of cadmium sulphate (3CdS04.8HzO) in water, adding 0.3 g of sodium hydroxide dissolved in water and finally making up the volume to 1 l., thus trapping all the H2S quantitatively as yellow CdS, as proposed by Morris et al (1957). The amount of sulphate in the raw and fermented samples was determined by the gravimetric method of Treadwell (1930) and Kolthoff and Sandell (1947), as used by Ghose (1955) in a modified procedure in which barium sulphate is precipitated with 5 per cent barium chloride solution. The apparatus used for the separation of fatty acids from the raw and fermented samples by steam-distillation and the estimation of the fatty acids were the same as those used by Ghose (1955) …”

Section: B Analytical Methodsmentioning

confidence: 99%

“…The amount of sulphate in the raw and fermented samples was determined by the gravimetric method of Treadwell (1930) and Kolthoff and Sandell (1947), as used by Ghose (1955) in a modified procedure in which barium sulphate is precipitated with 5 per cent barium chloride solution. The apparatus used for the separation of fatty acids from the raw and fermented samples by steam-distillation and the estimation of the fatty acids were the same as those used by Ghose (1955) …”

Section: B Analytical Methodsmentioning

confidence: 99%

“…Later, Baars (1 930) distinguished between two species, D. desulphuricans and D. rubentschickii, on the basis of their capacity to utilize fatty acids as hydrogen donors and, accordingly, the only species of sulphate reducers capable of metabolizing fatty acids was named D. rubentschickii. Ghose (1955) established the existence of an inhibitory action by lower fatty acids on the reduction of sulphates by the Zurich strain of D. desulphuricans. The degree of this inhibition increased with increase in the chain length of the acid up to the C4-acid.…”

Section: A Injeuence Of Volatile Acids In the Bacterial Sulphte Redmentioning

confidence: 97%

“…A trace of Mohr's salt was also used as before and the media incubated at 37°C. Culture tubes were sealed under alkaline pyrogallol, plugged, and incubated anaerobically according to Ghose (1955). Distilled water and reagent grade chemicals were used in the preparation of media in all cases.…”

Section: -mentioning

confidence: 99%

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Bacterial sulphide production from sulphate‐enriched spent distillery liquor—II

Basu

Ghose

1961

Biotechnol. Bioeng.

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Summary. Accumulation of volatile acids produced during anaerobic fermentation of distillery-spent liquor decreased the activity of Desulphovibrio desulphuricans employed in previously communicated studies; this caused a decrease in the reduction of sulphates to sulphides. An enriched strain of Desulphovibrio rubentschikii made in a synthetic mineral medium (NH4C1, 1.0 g ; MgS04.7H20, 2.0 g ; NaCl, 10.0 g ; CaS04, 1.0 g; calcium acetate, 1.0 g ; K2HP04, 0.5 g; Mohrs' salt, trace in 1 1.) using calcium acetate as the substrate and carbon from the tap-water was gradually built up to a 3-1. volume. The amount of hydrogen sulphide in this medium was found to be 225 mg/l. The fermentation was next carried out in a synthetic medium containing volatile acids from distillery-spent liquor as the only substrate. A 3-1. fermentation set up with diluted distillery-spent liquor (300 ml of raw liquor in 3,000 ml of water), neutralized with NaOH to pH 7.2 and supplemented with calcium sulphate and 0.1 per cent urea, showed sulphide productions (calculated as milligrams of hydrogen sulphide per litre of raw distillery-spent liquid) of 2,000, 4,000 and 6,000 with 0.1, 1.0 and 2.0 per cent calcium sulphate in the charge respectively. The volatile acid content, expressed as acetic acid, was 18,564 mg/litre of raw distillery-spent liquor on an average throughout the study. The ratio of hydrogen sulphide produced per litre of raw distillery-spent liquor to the amount of volatile acids developed expressed in milliequivalents per litre of raw liquor during fermentation increased gradually from 0.1 367 to 0.5567 in the presence of 0.1 to 2-0 per cent of calcium sulphate in the charge. PrefaceIn a previous communication (Part I, in press), studies carried out with organisms of the type Desulp?wvibrio desulphuricans in the bacterial reduction of sulphates to sulphides using distillery -spent liquor as carbon and hydrogen donor were reported. It was pointed out that increased yields of sulphide might be obtained by employing in the microbial sulphate-reduction a volatile-acid-utilizing 181 182 S. K. BASU AND T. K. GHOSE bacteria of the type Desulphovibrio rubentschickii. Studies subsequently conducted on the enrichment, isolation and fermentation characteristics of a specific volatile-acid-utilizing strain of sulphate reducers isolated from Calcutta City Sewage are reported. A comparative study on the sulphide fermentation with the agency of both D . desulphuricans and D . rubentschickii using distillery-spent liquor as hydrogen-donor has also been made. The influence of volatile acids on the bacterial reduction of sulphates to sulphides is also reported here.

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Section: B Analytical Methodsmentioning

confidence: 99%

Section: B Analytical Methodsmentioning

confidence: 99%

Section: A Injeuence Of Volatile Acids In the Bacterial Sulphte Redmentioning

confidence: 97%

Section: -mentioning

confidence: 99%

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Bacterial sulphide production from sulphate‐enriched spent distillery liquor—II

Basu

Ghose

1961

Biotechnol. Bioeng.

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Carbon balance in high rate dissimilation of acetic acid in semicontinuous methane fermentation

Ghose¹,

Gupta²,

Bhattacharyya³

1962

Biotech & Bioengineering

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SummaryThe nature of anaerobic methane fermentation has been studied extensively over a period of about three years by means of 10-1. semicontinuous fermenters, u-ith ethyl alcohol and calcium acetate as carbon and energy sources. Thc inoculum for the two systems was isolated by enrichment techniques from primary sewage sludge and used to initiat.e the processes. No subsequent seeding was necessary. The fermentation was started with a substrate carbon concentration of 50 mmole/l. which u-as gradually increased to 400 mmole/l.; this means that an increase of substrate level from 3000 ppm to 24,000 ppm of acetate and from 2300 to 18400 ppm of alcohol medium were effected during the study. Such high acetate tolerance of the methane organism does not appear to have been reported before; it was the result of adaptation by the organisms in a aemicontinuous culture for a period of 30 months. Effect of supplementary available nitrogen on t.he rate of gas production at different carbon levels (acetat.e) was also studied. It was found from the gas analyses that CH4:COz ratios were always more than 2 to 3 times the theoretical values, when acetate was used as substrate carbon. At some stages of acetate breakdown the concentration of hydrogen in the gaseous products was found to be as high as 8-9O/cl a very significant feature of this ferment,ation. Although sulphate ions were carefully excluded from the constituents of the media (Analytical reagent chemicals and double-distilled water used throughout) during the period of study (30 months), contaminants later identified as Desulphovibrio desulphuricans were found throughout the study. Morphological study of the organisms reveal that they are short rods appearing in individual or chain form, highly motile, gram-positive and nonflagellated, similar to Methanobacterium siihngenii Barker.

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Formation of mackinawite by the microbial reduction of jarosite and its application to tidal sediments

Ivarson

Hallberg

1976

Geoderma

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The influence of short-chain fatty acids, vitamins and some other factors on the sulphate-reduction in lactate cultures ofDesulphovibrio desulphuricans (Beijerinck), Kluyver and van Niel

Cited by 3 publications

References 69 publications

Bacterial sulphide production from sulphate‐enriched spent distillery liquor—II

Bacterial sulphide production from sulphate‐enriched spent distillery liquor—II

Carbon balance in high rate dissimilation of acetic acid in semicontinuous methane fermentation

Formation of mackinawite by the microbial reduction of jarosite and its application to tidal sediments

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