The Influence of Feed-Supplementation with Probiotic Strain Lactobacillus reuteri CCM 8617 and Alginite on Intestinal Microenvironment of SPF Mice Infected with Salmonella Typhimurium CCM 7205

Abstract

Alginite is a non-ore raw material arising by fossilization of accumulated organic (algae) and inorganic material, particularly clay, carbonates, quartz, and amorphous modification of silicic acid in the aqueous environment. Humic acids as a component of organic portion of alginite are known for very good buffering ability which allows them to stabilise pH throughout the digestion system of animals, stimulate receptors of the immune system in intestinal villi against pathogenic bacteria, and support proliferation and activity of beneficial bacteria (lactobacilli, bifidobacteria, and similar). Our investigations focused on the influence of a probiotic strain in combination with alginite on intestinal microenvironment of SPF mice infected with Salmonella Typhimurium. The 66 female mice (BALB/c) used in our study were divided to four experimental groups, control NC1, control NC2 (alginite), IC (alginite + Salmonella Typhimurium CCM 7205 NAL ), LAB (Lact. reuteri CCM 8617 + alginite + Salm. Typhimurium CCM 7205 NAL ). The group supplemented with Lact.reuteri CCM 8617 and alginite showed significant reduction in growth of Salm. Typhimurium in mice faeces at 24 and 72 h (P < 0.001) post infection. The supplementation of additives affected positively also nitrogen, enzymatic, hepatic and energy metabolism of mice. The demonstrable positive influence of additives alleviated the negative impact of Salm. Typhimurium infection on the morphology investigated in the jejunum and ileum of LAB group of mice. The livers of mice treated with both alginite and Lact.reuteri CCM 8617 showed marked reduction of overall inflammation, hepatocyte necrosis and size of typhoid nodules.

Effects of Oral Administration of Bamboo (Dendrocalamus membranaceus) Leaf Flavonoids on the Antioxidant Capacity, Caecal Microbiota, and Serum Metabolome of Gallus gallus domesticus

“…strains lead to the accumulation of SCFAs, such as acetic acids and butyrate (55)(56)(57). A previous study conducted by Gancarčíková et al confirmed that the increase in the caecal SCFA concentrations (mainly in the butyric, acetic, and propionic acid concentrations) was induced by L. reuteri (58). In the present study, the BLF supplementation increased the relative abundance of L. acidophilus, L. gasseri, and L. reuteri compared to the Anti treatment, as well as the relative abundance of L. reuteri compared to the control treatment.…”

Lactobacillus acidophilus DDS-1 Modulates Intestinal-Specific Microbiota, Short-Chain Fatty Acid and Immunological Profiles in Aging Mice

“…A limited number of studies have reported that valeric acid may stimulate intestinal growth and ameliorate the pathogenesis of diseases ranging from colitis and cancer to cardio-metabolic diseases [48,49,50]. Moreover, a variety of animal and human studies have reported very low to undetectable concentrations of valeric acid in faeces, colon, cecum, and ileum [51,52,53,54]. Interestingly, in the present study, valeric acid levels were clearly detected and upregulated among mucosal samples in both DDS-1 treated groups and were positively correlated with an increase in Bacteroidetes level [52].…”

Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets

“…Leonardite is rich in humic acids, and due to its macrocolloid structure can protect intestinal mucosa by reducing the resorption of toxic metabolites from the residues of harmful substances in feed [25]. Humic acids prevent an excessive loss of water from the gut, which is important in the treatment of diarrhoea, dyspepsia, and acute intoxications [26].…”