The influence of exposure to Euphorbia splendens var. hislopii latex on the concentrations of total proteins and nitrogen products in Biomphalaria glabrata infected with Schistosoma mansoni

Mello-Silva, Clélia Christina; Vasconcellos, Maurício Carvalho de; Bezerra, José Clecildo Barreto; Rodrigues, Maria de Lurdes Azevedo; Pinheiro, Jairo

doi:10.1016/j.actatropica.2010.10.007

Cited by 22 publications

(16 citation statements)

References 17 publications

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“…This phenomenon leads to mobilization of the energy reserves in the tissues to the haemolymph, promoting glucose homeostasis (Bezerra et al, 1999). Similar physiological alterations are observed in snails submitted to other physiological stresses such as severe starvation, aestivation (Becker, 1980), exposure to natural molluscicides (Mello-Silva et al, 2010) and association of infection with molluscicides (Mello-Silva et al, 2011).…”

Section: Resultsmentioning

confidence: 81%

Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Faro

Perazzini

Corrêa

et al. 2013

Experimental Parasitology

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Parasitic castration in the snail-trematode relationship can be understood as any change in the reproductive function of the snail that is due to interference by the developing larvae inside the snail that leads to the reduction or complete disruption of egg-laying activity. This study was designed to observe the parasitic castration of Biomphalaria glabrata infected with Schistosoma mansoni during both the pre-patent and patent periods. The effect of infection on snail fecundity and fertility, growth rate and survival was studied during the 62 days following miracidia exposure. An integrated approach was employed that used biochemical and histological tools over the same period. To study the effect of infection on reproduction, we individually exposed 30 snails to 5 miracidia each and tracked their fertility and fecundity. For our histopathological studies, 50 snails were exposed to 20 miracidia each, and for our histochemical studies, 50 snails were exposed to 5 miracidia each. An equal number of uninfected snails were used as a control for each group. The B. glabrata exposed to the BH strain of S. mansoni showed 50% positivity for cercarial shedding. Both the experimental and control groups showed 100% survival. The pre-patent period lasted until 39 days after exposure to miracidia. Exposed snails that showed cercarial shedding exhibited higher growth rates than either exposed snails that did not demonstrate cercarial shedding or uninfected controls. Exposed snails without cercarial shedding and uninfected controls showed no differences in the reproductive parameters evaluated during the patent period; snails experiencing cercarial shedding showed a reduction in fecundity and fertility. These snails began to lay eggs only after the 50th day post miracidia exposure. The haemolymph glucose levels showed an oscillating pattern that decreased during periods of greater mobilisation of energy by the larvae and was accompanied by a depletion of glycogen in the cephalopodal mass and digestive gland. Histopathological examination at 55 days showed that the ovotestis was highly atrophied. There was almost complete disappearance of germ cells, and the supporting stroma formed a nearly empty net. At day 45, the infected digestive gland showed a high cylindrical epithelium with little preserved cytoplasm. The contents of the secretory granules of the albumen gland of infected animals stained with Alcian blue (AB), pH 1.0, indicating the presence of sulphated carbohydrates. Thus, parasitic castration in the B. glabrata-S. mansoni model may be regulated directly and indirectly by the developmental stage of the trematode and the biochemical and histopathological alterations during the patent period of infection.

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Section: Resultsmentioning

confidence: 81%

Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Faro

Perazzini

Corrêa

et al. 2013

Experimental Parasitology

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“…Other studies have shown that the application of substances derived from E. milii in water bodies in transmission areas leads to changes in physiological stocks and S. mansoni/B. glabrata compatibility [16][17][18]. The effects of E. milii latex on the parasite life cycle in both schistosomiasis hosts (snails and mammals) have been described.…”

Section: Introductionmentioning

confidence: 99%

Destruction of secondary Schistosoma mansoni sporocysts in Biomphalaria glabrata after phytochemical exposure

Friani

Costa

Mota

et al. 2020

Preprint

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Background: Schistosomiasis is a neglected tropical disease and affects over 200 million people worldwide. The snail Biomphalaria glabrata is one of the intermediate hosts of S. mansoni . The aim of this work was to verify the action of Euphorbia milii var. hislopii latex in the hemocytes profile and histopathology of B. glabrata infected by S. mansoni .Methods: Uninfected and infected snails were exposed to sublethal concentrations of E. milii latex for 24 hours (1.0 mg/l less than the LC 50 ) and after this time were analysed.Results: The survival rate was 88.5% for the uninfected snails and 66.6% for the infected and exposed snails. In the snails infected by S. mansoni , the exposure to E. milii latex promoted proliferation of hemocytes in the tentacles, mantle, digestive gland, kidney and ovotestis. In the digestive gland and the kidney, granulomatous reactions occurred around the sporocysts and caused their destruction. Proliferation of hemocytes in the kidney and digestive gland and edema in the mantle area were also observed in the uninfected and exposed snails. The number of circulating hemocytes from the group infected and exposed to E. milii latex was significantly higher than in the other groups. Three types of hemocytes were found: hyalinocytes, granulocytes and blast-like cells, and in all the groups the proportion of hyalinocytes was higher than the other types. There was no significant difference among the cell types and the different groups analyzed. Conclusions: We conclude that the sublethal concentration of E. milii latex influenced the cellular immune response of the susceptible B. glabrata strain to infection by S. mansoni , promoting the destruction of parasites.

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“…It is active against mollusks which are intermediate hosts of Schistosomatrematodes at concentrations as low as 0.5 ppm in the laboratory and at concentrations as low as 5.0 ppm under field conditions (Vasconcellos&Schall, 1986;Mendes et al, 1992). Besides its remarkable potency, E. milii latex also presents other advantages as a molluscicide: E. milii can be easily cultivated in endemic areas and yields a rather large amount of latex throughout the year, no marked seasonal nor geographic variation have been found in the molluscicidal activity, and the latex has proved to be less harmful to nontarget organisms than niclosamide, today's mostly used synthetic molluscicide (Baptista et al, 1994;Oliveira-Filho et al, 2000;Yadav &Jagannadham, 2008;Oliveira-Filho et al, 2010;Mello-Silva et al, 2011).…”

Section: _164mentioning

confidence: 99%

Effects of Euphorbia milii latex on mitogen-induced lymphocyte proliferation

Delgado

Paumgartten

2014

Rev. bras. plantas med.

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ABSTRACT:The crude latex of "Crown-of-Thorns" (Euphorbia milii var hislopii, syn E.splendens) is a potent plant molluscicide. For this reason, toxicological studies have been performed to evaluate the health risks posed by its use in schistosomiasis control programs. The present study is part of a more comprehensive immunotoxicological evaluation of this molluscicide. Here, we investigated the effects of E. milii latex on the proliferation of human lymphocytes in vitro. Lyophilized latex of E. milii (0, 0.5, 5, 25 and 50 µg/ml) was incubated with whole blood in the presence of proliferation stimulators, i.e. lectins (phytohemagglutinin, concanavalin A and pokeweed mitogen), as well as with human monoclonal antibody against CD3 and tetanus toxoid. Cell proliferation was measured by 3 H-thymidine incorporation, and the effects of latex on mitogen-induced cell proliferation were compared to the effects of 10 ng/ ml of 12-O-tetradecanoylphorbol-13-acetate (TPA). Results showed that mitogen-induced cell proliferation was markedly enhanced by E. milii latex. This synergistic effect of latex on mitogeninduced lymphocyte proliferation may be due to the presence of TPA-like phorbol esters and/ or to mitogenic plant lectins.

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The influence of exposure to Euphorbia splendens var. hislopii latex on the concentrations of total proteins and nitrogen products in Biomphalaria glabrata infected with Schistosoma mansoni

Cited by 22 publications

References 17 publications

Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Destruction of secondary Schistosoma mansoni sporocysts in Biomphalaria glabrata after phytochemical exposure

Effects of Euphorbia milii latex on mitogen-induced lymphocyte proliferation

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