The in vitro metabolism of estrone and estradiol-17β and their 3-sulfates by the renal tissues from pregnant and female fetal guinea-pigs

Adessi, G. L.; Nhuan, Tran Quang; Prost, O.

doi:10.1016/0022-4731(81)90314-9

Cited by 3 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2c) (Adessi et al, 1981 M-oestradiol-17ß, these values reached 177-2 (19-4-565-3) and 62-9 (7-5-204T) respectively.…”

Section: Morphological Resultsmentioning

confidence: 87%

“…Conversely, a soluble sulphotransferase converts intracellular free oestrogens into their conjugated derivatives which are inactive metabolites . Moreover, the conversion of oestrone sulphate to oestradiol sulphate must arise without prior removal of the sulphate moiety and not by hydrolysis of the oestrogen sulphates followed by resulphurylation (Adessi et al, 1981).…”

Section: Morphological Resultsmentioning

confidence: 99%

“…Laure & Pasqualini (1983) have demonstrated that oestradiol-17ß increased the RNA polymerase in fetal uterus. Moutaouakkil, Prost, Dahan & Adessi (1984) have studied sulphatase activities in the uterus, and a metabolic pathway of oestrogens in pregnant female guinea-pigs has been proposed (Adessi, Tran Quang Nhuan & Prost, 1981;Adessi, Tran Quang Nhuan & Vingler, 1982).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Culture of epithelial and stromal cells of guinea-pig endometrium and the effect of oestradiol-17 on the epithelial cells

Chaminadas¹,

Propper²,

Royez³

et al. 1986

Reproduction

View full text Add to dashboard Cite

“…2c) (Adessi et al, 1981 M-oestradiol-17ß, these values reached 177-2 (19-4-565-3) and 62-9 (7-5-204T) respectively.…”

Section: Morphological Resultsmentioning

confidence: 87%

Section: Morphological Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Culture of epithelial and stromal cells of guinea-pig endometrium and the effect of oestradiol-17 on the epithelial cells

Chaminadas¹,

Propper²,

Royez³

et al. 1986

Reproduction

View full text Add to dashboard Cite

Effect of progesterone on protein synthesis and secretion by cultured epithelial cells from guinea-pig endometrium

et al. 1989

View full text Add to dashboard Cite

The pattern of proteins synthesized and secreted in response to progesterone by guinea-pig endometrial epithelial cell cultured with estradiol-17 beta was investigated. Glandular epithelial cells were maintained in culture for 3 days on growth medium, then washed three times with a steroid-free medium. After this period, 2 x 10(-8) M estradiol-17 beta or 2 x 10(-8) M estradiol-17 beta plus 5 x 10(-7) M progesterone were added to the medium for 48 h. To study biochemical changes, the proteins in medium and in cells were analyzed by one- and two-dimensional polyacrylamide gel electrophoresis, followed by fluorography. The addition of progesterone to estradiol-17 beta in the culture medium caused a change in the patterns of cellular and secreted proteins: one-dimensional gel electrophoresis showed that variation of 8 cellular proteins and 12 secreted proteins was caused by progesterone. Induction of individual proteins by progesterone treatment was observed by two-dimensional gel electrophoresis: one cellular protein (Mr 49,000; pI 5.90) and one secreted protein (Mr 14,300; pI 4.80) were specifically induced and might serve as markers of progesterone action.

show abstract

Comparison of Placental HSD17B1 Expression and Its Regulation in Various Mammalian Species

Yazawa

Islam

Imamichi

et al. 2023

Animals

View full text Add to dashboard Cite

During mammalian gestation, large amounts of progesterone are produced by the placenta and circulate for the maintenance of pregnancy. In contrast, primary plasma estrogens are different between species. To account for this difference, we compared the expression of ovarian and placental steroidogenic genes in various mammalian species (mouse, guinea pig, porcine, ovine, bovine, and human). Consistent with the ability to synthesize progesterone, CYP11A1/Cyp11a1, and bi-functional HSD3B/Hsd3b genes were expressed in all species. CYP17A1/Cyp17a1 was expressed in the placenta of all species, excluding humans. CYP19A/Cyp19a1 was expressed in all placental estrogen-producing species, whereas estradiol-producing HSD17B1 was only strongly expressed in the human placenta. The promoter region of HSD17B1 in various species possesses a well-conserved SP1 site that was activated in human placental cell line JEG-3 cells. However, DNA methylation analyses in the ovine placenta showed that the SP1-site in the promoter region of HSD17B1 was completely methylated. These results indicate that epigenetic regulation of HSD17B1 expression is important for species-specific placental sex steroid production. Because human HSD17B1 showed strong activity for the conversion of androstenedione into testosterone, similar to HSD17B1/Hsd17b1 in other species, we also discuss the biological significance of human placental HSD17B1 based on the symptoms of aromatase-deficient patients.

show abstract

The in vitro metabolism of estrone and estradiol-17β and their 3-sulfates by the renal tissues from pregnant and female fetal guinea-pigs

Cited by 3 publications

References 9 publications

Culture of epithelial and stromal cells of guinea-pig endometrium and the effect of oestradiol-17 on the epithelial cells

Culture of epithelial and stromal cells of guinea-pig endometrium and the effect of oestradiol-17 on the epithelial cells

Effect of progesterone on protein synthesis and secretion by cultured epithelial cells from guinea-pig endometrium

Comparison of Placental HSD17B1 Expression and Its Regulation in Various Mammalian Species

Contact Info

Product

Resources

About