Effect of progesterone on protein synthesis and secretion by cultured epithelial cells from guinea-pig endometrium

Abstract

The pattern of proteins synthesized and secreted in response to progesterone by guinea-pig endometrial epithelial cell cultured with estradiol-17 beta was investigated. Glandular epithelial cells were maintained in culture for 3 days on growth medium, then washed three times with a steroid-free medium. After this period, 2 x 10(-8) M estradiol-17 beta or 2 x 10(-8) M estradiol-17 beta plus 5 x 10(-7) M progesterone were added to the medium for 48 h. To study biochemical changes, the proteins in medium and in cells were analyzed by one- and two-dimensional polyacrylamide gel electrophoresis, followed by fluorography. The addition of progesterone to estradiol-17 beta in the culture medium caused a change in the patterns of cellular and secreted proteins: one-dimensional gel electrophoresis showed that variation of 8 cellular proteins and 12 secreted proteins was caused by progesterone. Induction of individual proteins by progesterone treatment was observed by two-dimensional gel electrophoresis: one cellular protein (Mr 49,000; pI 5.90) and one secreted protein (Mr 14,300; pI 4.80) were specifically induced and might serve as markers of progesterone action.

Estrous-Cycle-Related Changes in the Expression of Mouse Endometrial and Oviductal Glycoproteins

“…Thus the 25-kD diestrus-related RCA-I-reactive endometrial mouse glycopro tein could be equivalent to the 30-kD rat protein induced by estrogen and progesterone treatment [20]. The 138-kD estradiol-induced guinea pig [11] secretory protein could be equivalent to the 135-kD glycoprotein from the present study. The 115-kD secretory protein detected in estrus by Jacobs and Little [9] in the rat uterus, which is suppressed under the influence of progesterone, probably corre sponds to the 115-kD estrus-phase-specific mouse glyco protein described in the present study.…”

“…Uterine and oviductal proteins secreted into the lumen of the nonpregnant and pregnant genital tract have been studied in several species, including rats [9], mice [4,10], hamsters [3], guinea pigs [11], pigs [ 12], sheep [ 13], cows [14] and baboons [15]. It appears that most of these pro teins are glycosylated, and it is assumed that they are hor monally regulated and related to the reproductive func tions of the uterus and the oviduct.…”

Glycoproteins of mouse vaginal epithelium: differential expression related to estrous cyclicity.

“…This study has expanded our previous lectin histochemical data and shows that the pattern of glycoproteins displayed by the mouse vag- has been widely used to study reproductive events in many species, such as guinea pigs (Chaminadas et al, 1989). hamsters (Leavitt et al, 1985).…”

Changes related to the oestrous cycle in the expression of endometrial and oviductal proteins of mice

“…The synthesis of the 115 kDa protein in the rat endometrium is inhibited by progesterone, which would correspond to our inability to detect it in dioestrus. It is also possible that our protein number 9 (Mr41, pi represents the mouse equivalent of the hamster uterine protein of the same molecular weight, described as number 9 by Chaminadas et al (1989). This protein appears only after combined oestrogen and progesterone treatment of cultured guinea-pig endometrium.…”