Culture of epithelial and stromal cells of guinea-pig endometrium and the effect of oestradiol-17  on the epithelial cells

Ultrastructural changes induced by oestradiol-17β, progesterone and oestrone-3-sulphate in guinea-pig endometrial glandular cells grown in primary culture

“…Standard food enriched with ascorbic acid was available ad libitum. Mature virgin females were killed on the day of vaginal opening, and uteri removed and placed in sterile HBSS as described by Chaminadas et al (1986).…”

“…Endometrial cell culture conditions Cells were prepared according to the method of Chaminadas et al (1986) as modified by Alkhalaf et al (1987o). Briefly, the endometria were dissociated by collagenase (0-25%, w/v) digestion for 2 h at 37°C.…”

“…Cell culture has provided a powerful tool for studying many aspects of cell growth and function under controlled conditions. Although hormone sensitivity of endometrial cells in primary culture is well documented (Gerschenson, Conner, Yang & Andersson, 1979;Chaminadas, Propper, Royez et al 1986;Alkhalaf, Chaminadas, Propper & Adessi, 1987 ), the effects of ovarian steroids on ultrastructural features of cultured endometrial cells have been rarely reported Propper & Adessi, 1987e). A direct action of oestrone sulphate on protein synthesis in rat and rabbit uterus has been demonstrated (Brooks, Leithauser, De Loecher & De Wever, 1969) and the prehormone role of oestrone sulphate has been established in different species and tissues (Payne, Laurence, Foster & Jaffe, 1973;Adessi, Tran & Vingler, 1982;Moutaouakkil, Prost, Dahan & Adessi, 1984;Prost, Turrel, Dahan et al 1984).…”

Effect of progesterone on proteins vectorially secreted by glandular epithelial cells of guinea-pig endometrium: modulation by homologous stroma

“…The establishment of glandular epithelial cell cultures has attracted considerable interest in various species including humans (Bongso et al, 1988;Rinehart et al, 1988;White et al, 1990) and guinea-pigs (Chaminadas et al, 1986;Alkhalaf et al, 1987Alkhalaf et al, , 1991 providing nourishment for the embryo before implantation (Roberts and Bazer, 1988;Beier et al, 1991). Progesterone is required to prepare the endometrium for implantation and progesterone-dependent proteins, synthesized and secreted by the endometrial gland cells, could be used as biochemical markers for the receptive state of the endometrium.…”

“…The endometrium is an epithelium/mesenchyme stromal structure that has an active role in producing autocrine and paracrine factors that modulate the physiology of steroid-dependent tissue (Inaba et al, 1988;Brigstock et al, 1989; Tabibzadeh, 1991). It has been suggested that at the time of blastocyst attachment the uterine epithelium may influence stromal decidualization via soluble factors (Lejeune et al, 1981;Tamada et al, 1990Tamada et al, , 1991 (Inaba et al, 1988;Hohn et al, 1989) (Glasser et al, 1988) and rabbits (Mani et al, 1991) (Chaminadas et al, 1986) and cultured and subcultured as decribed by Mahfoudi et al (1992) Mahfoudi et al (1991a).…”