The Importance of Antibody Concentration, Binding Constant, and Heterogeneity in the Suppression of Immunity to the Rh Factor

The Importance of Equilibrium Constant and Heterogeneity Index on Anti-Rh(0)(D) Quantitation Using the Auto-Analyser

“…A non-symmetrical distribution of binding energies could be interpreted as symmetrical if the proportion of bound antibody molecules were small and represented the material only with high equilibrium constant. However, the degree of asymmetry in the Rh0(D) -anti-Rho reaction must be small since Hughes-Jones [8] using antiserum, and Pollack and Kochesky [21] using immunoglobulin found a symme trical distribution when some 75 and 95%, respectively, of the antibody had been bound. It has been assumed, therefore, for the purpose of the calcula tions presented in this paper that the Sips equation effectively describes the antigen-antibody reaction in the Rh blood group system.…”

“…This condition is more readily complied with in the assay of anti-Rho(D) immunoglobulin since the equilibrium constants of different pools will tend to be similar [9,14,21]. The use of a single, pooled standard preparation for the anti-Rh0(D) assay in individual antisera has distinct advantages.…”

Assay of Anti-D Activity by Two Radioimmunoassay Techniques Using Purified 125I-Labelled Anti-D

“…The most accurate method that is available at the present time is the method of direct labelling of the IgG immunoglobulin containing the anti-D with 126I, the absorption of the labelled anti-D onto red cells and the analysis of the results by Scatchard's graphical analysis [6,8], A modification of this method is the assay of anti-D bound to red cells using 125I-labelled anti-IgG globulin [5] but the accuracy of this method in part rests on the assumption that the anti-IgG combines to the same extent with all preparations of anti-D. Since this may not be true, it is desirable to have an alternative method of assay.…”

Solid Phase Radioimmunoassay for Quantitating Anti-HB_S in Hepatitis B Immune Globulin

“…This report describes a modi fication of the solid-phase radioimmune assay of Cat! and Tregear [2] that allows the data to be resolved into a Sips adsorption isotherm and which has been used successfully for quantitating anti-D immune globulin [3]. This treatment of the data permits the total antibody, intrinsic average binding constant and index of heterogeneity to be determined since we could demonstrate that the antibody behaves as if it is monovalent with respect to binding to its antigen.…”