Solid Phase Radioimmunoassay for Quantitating Anti-HB_S in Hepatitis B Immune Globulin

Abstract

A solid phase radioimmunoassay was used to quantitate the antibody in hepatitis B immune globulin. Polystyrene tubes coated with HB_sAg were incubated with several dilutions of trace-labeled immune globulin, and the specific uptake was measured. Resulting data could be used to obtain the total antibody concentration in micrograms per milliliter as well as the intrinsic binding constant and index of heterogeneity.