The implementation of a rapid sample preparation method for the detection of SARS-CoV-2 in a diagnostic laboratory in South Africa

Marais, Gert; Naidoo, Michelle; Hsiao, Nei Yuan; Valley‐Omar, Ziyaad; Smuts, Heidi; Hardie, Diana

doi:10.1371/journal.pone.0241029

Cited by 18 publications

(14 citation statements)

References 10 publications

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“…The genetic signature can be quickly identified consistent with the novel coronavirus. Marais et al [ 35 ] proposed a rapid sample preparation method based on liquid handling robots to detect the SARS-CoV-2 virus. Similarly, the sensitive and specific detection of SARS-CoV-2 antibodies is also taken on an automated liquid-handling robotic system [ 36 ].…”

Section: Robots In Covid-19mentioning

confidence: 99%

A literature survey of the robotic technologies during the COVID-19 pandemic

Wang

2021

Journal of Manufacturing Systems

185

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Since the late 2019, the COVID-19 pandemic has been spread all around the world. The pandemic is a critical challenge to the health and safety of the general public, the medical staff and the medical systems worldwide. It has been globally proposed to utilise robots during the pandemic, to improve the treatment of patients and leverage the load of the medical system. However, there is still a lack of detailed and systematic review of the robotic research for the pandemic, from the technologies’ perspective. Thus a thorough literature survey is conducted in this research and more than 280 publications have been reviewed, with the focus on robotics during the pandemic. The main contribution of this literature survey is to answer two research questions, i.e. 1) what the main research contributions are to combat the pandemic from the robotic technologies’ perspective, and 2) what the promising supporting technologies are needed during and after the pandemic to help and guide future robotics research. The current achievements of robotic technologies are reviewed and discussed in different categories, followed by the identification of the representative work’s technology readiness level. The future research trends and essential technologies are then highlighted, including artificial intelligence, 5 G, big data, wireless sensor network, and human-robot collaboration.

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Section: Robots In Covid-19mentioning

confidence: 99%

A literature survey of the robotic technologies during the COVID-19 pandemic

Wang

2021

Journal of Manufacturing Systems

185

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“…NP, nasopharyngeal; VTM, viral transport medium; NA, not available. b The CN values are specific values reported by the Abbott RealTime SARS CoV-2 assay and not directly comparable to C T values ( 17 ). …”

Section: Resultsmentioning

confidence: 99%

Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing

et al. 2021

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Combating the ongoing coronavirus disease 2019 (COVID-19) pandemic demands accurate, rapid, and point-of-care testing with fast results to triage cases for isolation and treatment. The current testing relies on RT-PCR, which is routinely performed in well-equipped laboratories by trained professionals at specific locations. However, during busy periods high numbers of samples queued for testing can delay the test results, impacting upon efforts to reduce the infection risk. Besides, the absence of well-established laboratories at remote sites and low-resourced environments can contribute to a silent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). These reasons compel the need to accommodate point-of-care testing for COVID-19 that meets the ASSURED criteria (Affordable, Sensitive, Specific, User-friendly, Rapid, robust, Equipment-free, and Deliverable). This study assessed the agreement and accuracy of the portable Biomeme SARS-CoV-2 system against the gold standard tests. Nasopharyngeal and nasal swabs were used. Of the 192 samples tested using the Biomeme SARS-CoV-2 system, the results from 189 samples (98.4%) were in agreement with the reference standard-of-care RT-PCR testing for SARS-CoV-2. The portable system generated simultaneous results for nine samples in 80 mins with high positive and negative percent agreements of 99.0% and 97.8%, respectively. We performed separate testing in a sealed glove box, offering complete biosafety containment. Thus, the Biomeme SARS-CoV-2 system can help decentralize COVID-19 testing and offer rapid test results for patients in remote and low-resourced settings. Single Sentence Summary The Biomeme SARS-CoV-2 system can help decentralize COVID-19 testing and offer rapid test results for patients in remote sites and low-resourced settings.

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“…Nasopharyngeal swabs were sent in a protected container directly to the laboratory for COVID-19 RT-PCR tests with Abbott RealTime SARS-CoV-2 Assay from Abbott Molecular Inc, (Salt Lake City, IL, USA) according to the manufacturer's instructions [ [16] , [17] , [18] ]. ICAM-1 serum levels were measured 1-week before and after debridement according to the protocol in the Human sICAM-1 ELISA Kit (Merck Millipore, cat.…”

Section: Methodsmentioning

confidence: 99%

ICAM-1 levels in patients with covid-19 with diabetic foot ulcers: A prospective study in southeast asia

Oley

Kepel

et al. 2021

Annals of Medicine &Amp; Surgery

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Background Viral infection can compound the severity of pre-existing inflammation caused by underlying diseases. For those with a chronic, immune-related condition such as diabetic foot ulcers (DFUs), the coronavirus disease (COVID-19) serves to exacerbate the inflammatory burden. Serum levels of intracellular adhesion molecule-1 (ICAM-1), a primary mediator of cell adhesion express in the inflammatory process, are often used to indicate the gravity of all inflammatory conditions. Therefore, the purpose of this study was to investigate serum ICAM-1 levels before and after debridement in patients with DFUs who were also diagnosed as COVID-19 positive compared with those who were COVID-19 negative. Methods 20 patients with DFUs were screened for COVID-19 and then divided into COVID-19 positive and negative groups according to the results. Before debridement, chest x-rays and blood analysis, including ICAM-1 serum levels, were performed in both groups. Only ICAM-1 serum levels were measured after debridement. Results Of the 20 patients included in this study, 55% were male (n = 11) and 45% were female (n = 9). The mean age was 52.9 ± 1.9 years. ICAM-1 levels in patients with DFU in the COVID-19-positive group were significantly higher than those in the COVID-19-negative group (median 317.2 vs 149.2, respectively; p < 0.001). Serum levels of ICAM-1 reduced significantly in patients with DFU in the COVID-19-positive group were significantly higher than those in the COVID-19-negative group after debridement (median 312.5 vs 130.3; p < 0.001). Conclusion ICAM-1 serum levels represent an additional, initial screening marker for COVID-19.

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The implementation of a rapid sample preparation method for the detection of SARS-CoV-2 in a diagnostic laboratory in South Africa

Cited by 18 publications

References 10 publications

A literature survey of the robotic technologies during the COVID-19 pandemic

A literature survey of the robotic technologies during the COVID-19 pandemic

Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing

ICAM-1 levels in patients with covid-19 with diabetic foot ulcers: A prospective study in southeast asia

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