The impact of rhubarb polysaccharides on Toll-like receptor 4-mediated activation of macrophages

Activation of macrophages by the ophiopogon polysaccharide liposome from the root tuber of Ophiopogon japonicus

“…In this experiment, we only investigated the effect of OPL on activating macrophages, the mechanism of action had not been studied yet. Many researches have already demonstrated that polysaccharide could activate various immune cells through toll-like receptors, and further activate intracellular signaling pathways, then promote the secretion of cytokines [19,29,30]. Therefore, we speculate that OPL also could activate macrophages through toll-like receptors, and then plays a role in immune regulatory effect.…”

Preparation and optimization of ophiopogon polysaccharide liposome and its activity on Kupffer cells

“…In this experiment, we only investigated the effect of OPL on activating KCs, the mechanism of action had not been studied yet. Many researches have already demonstrated that polysaccharide could activate various immune cells through toll-like receptors, and further activate intracellular signaling pathways, then promote the secretion of cytokines (Huang et al, 2014;Kim et al, 2013;Zhang et al, 2013). Therefore, we speculate that OPL also could activate KCs through toll-like receptors, and then plays a role in immune regulatory effect.…”

Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation

“…Assay of mRNA content of IL-2, IL-10, IFN-γ, CD4 and CD8 in spleen by RTFQ-PCR Determination of mRNA content of IL-2, IL-10, IFN-γ, CD4 and CD8 in spleen was carried out by RTFQ-PCR according to the reported method with slight modification (Liao & Lin, 2013;Zhang et al, 2013). Toll RNA was extracted by Column Animal total RNA Purification Kit, then, cDNA was synthesized by M-MuLV First Strand cDNA Synthesis Kit in peltier thermal cycler (PTC-200, Bio-RAD, USA).…”