The immunopotentiating effects of shark-derived protein hydrolysate

Mallet, Jean‐François; Duarte, Júlio César; Vinderola, Gabriel; Anguenot, Raphaël; Beaulieu, Martin; Matar, Chantal

doi:10.1016/j.nut.2013.10.025

Cited by 39 publications

(30 citation statements)

References 49 publications

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“…On the other hand, 80 μg mL −1 AH and NH treatments produced higher concentrations of IL‐10 among other treatments, showing that even if the pro‐inflammatory cytokines produced by AH and NH overexpress, it should still have an anti‐inflammatory effect to maintain homoeostasis. In line with this, the IL‐4, IL‐6, and IL‐10 levels were all increased in lamina propria of the small intestine of mice when fed by fish protein concentrate (Duarte et al ., ); shark‐derived protein hydrolysates also kept a balance with pro‐inflammatory and anti‐inflammatory intestinal homoeostasis (Mallet et al ., ). However, to ascertain the pathways by which AH and NH produce such cytokines and how long they can be administered for optimal homoeostasis, the further investigation needs to be conducted.…”

Section: Resultsmentioning

confidence: 86%

“…() reported that milk whey hydrolysed by alcalase shows significantly higher level ( P < 0.05) than that hydrolysed by neutrase and pepsin in production of TNF‐α and IL‐12. Shark protein hydrolysate (prepared by trypsin–chymotrypsin) stimulated higher level of IL‐6 than that produced by unhydrolysed fish proteins, but lower than that produced by LPS (Mallet et al ., ).…”

Section: Resultsmentioning

confidence: 97%

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Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum Vulgare) globulin

Zhang

Sun

et al. 2016

Int J of Food Sci Tech

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Summary Defatted wheat germ globulin (DWGG) was hydrolysed using the enzymes alcalase, neutrase, papain, pepsin or trypsin to improve the immunomodulatory effects of the protein. The degree of hydrolysis (DH), molecular weight distribution, hydrophobicity, secondary structures and immunomodulatory activities were determined. The DH of hydrolysate using alcalase or neutrase was higher (P < 0.05) than that of hydrolysate prepared by other proteases. Alcalase hydrolysate (AH) had the strongest (P < 0.05) immunomodulatory activity with respect to lymphocyte proliferation, phagocytosis of neutral red and secretion of pro‐inflammatory cytokines. The molecular weight of AH ranged between 300 and 1450 Da and was composed of α‐helix and β‐turns. AH also showed the highest hydrophobic value (P < 0.05). The DH and hydrophobicity appear to be related to immunomodulatory activities. The hydrolysate prepared by the alcalase treatment on DWGG may be used as an immune supplement by the food industry.

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 97%

Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum Vulgare) globulin

Zhang

Sun

et al. 2016

Int J of Food Sci Tech

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“…The peptides isolated from chicken bursa can promote the development of B cells and the production of specific antibody and cytokines . Shark protein hydrolysate shows immune effect via up-regulating the IgAproducing cells and the secretion of IL-6 and TNF-α (Mallet et al, 2014). Pine nut meal protein peptides with molecular weights of 3-10 kDa have immuno-competence in the mice by enhancing macrophage phagocytosis and phagocytosis index, and by increasing the production of four cytokines significantly (Lin, Liu, Liu, & Yu, 2017).…”

Section: Discussionmentioning

confidence: 99%

In vitro immuno-modulatory ability of tryptic caseinate hydrolysate affected by prior caseinate glycation using the Maillard reaction or transglutaminase

Song

Zhao

2017

Food and Agricultural Immunology

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Caseinate was glycated by lactose and oligochitosan via the Maillard reaction and transglutaminase catalysis to yield two glycated caseinates (GC-I and GC-II), followed by trypsin hydrolysis to generate GC-I hydrolysate and GC-II hydrolysate, respectively. In comparison with caseinate hydrolysate, only GC-I hydrolysate showed decreased His, Lys, Tyr, and Val contents. All three hydrolysates in dose-dependent manner exerted in vitro immunomodulatory abilities via activating three immune cells in terms of lymphocytes, macrophages, and natural killer (NK) cells, enhancing the secretion of seven cytokines in lymphocytes and macrophages, but inhibiting the secretion of one cytokine (interleukine-4) in splenocytes. However, GC-I hydrolysate and GC-II hydrolysate showed respective lower and higher immuno-modulatory abilities than caseinate hydrolysate in the most cases. Amino acid losses of GC-I hydrolysate made partially contribution to its impaired immuno-modulatory ability. It is concluded that lactose glycation and oligochitosan glycation of caseinate could lessen and enhance immuno-modulation of tryptic caseinate hydrolysate, respectively. ARTICLE HISTORY

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“…Novel peptides isolated and characterized from the chicken bursa of Fabricius promoted the development of mice B cell and enhanced the production of specific Avian Influenza Virus antibody and cytokines, T-cell immunophenotyping at reachable concentrations (Liu et al, 2013). Shark-derived protein hydrolysate digested by trypsin and type II α-chymotrypsin-enhanced mice gut barrier function by upregulating IgA-producing cells and the protein level of intestinal interleukin-6 and tumor necrosis factor-α and downregulating of uncontrolled-inflammatory reaction induced by Escherichia coli infection via increasing transforming growth factor-β and interleukin-10 (Mallet et al, 2014). According to our previous research, Coix seed was rich in protein (14.17%) (Wang, Yuan, Zhang, & Qiao, 2012); therefore, it is worth exploring immune peptides or protein from Coix seed.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

et al. 2016

CyTA - Journal of Food

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2017)Immunomodulatory activity of small molecular (≤3 kDa) Coix glutelin enzymatic hydrolysate, CyTA -Journal of Food, 15:1, 41-48, ABSTRACT In this study, small molecular (≤3 kDa) Coix glutelin hydrolysate (CGH) was obtained by the method of enzymatic hydrolysis and then immunomodulatory activity was evaluated in vitro and in vivo. Stimulation index of mice splenocytes was increased in a dose-dependent manner after treated with 3.125-25 μg/mL CGH. Acid phosphatase in mice peritoneal macrophages was significantly improved after stimulated with CGH at some concentration. The CGH stimulated the secretion of NO from Raw264.7 in a concentration-dependent manner and also significantly inhibited lipopolysaccharideinduced cell excessive activation at some extent. After successive administration of CGH for 21 days, the spleen index of ICR was significantly increased to 3.73 mg/g for the group administrated 800 mg/kg CGH. Malondialdehyde (MDA) assay displayed that 200 mg/kg CGH increased mice serum MDA concentration, while the variation was obsolete in the other CGH groups. All results demonstrated that CGH took essential effects on immunomodulatory activities.Actividad inmunomoduladora de hidrolisato glutelina encimático de moléculas pequeñas de Coix (≤ 3 kDa) RESUMEN En este estudio, se obtuvo hidrolisato glutelina de Coix (CGH) de molécula pequeña (≤ 3 kDa) mediante el método de hidrólisis enzimática y después se evaluó la actividad sinmunomoduladora in vitro y in vivo. El índice de estimulación (SI) de los esplenocitos de ratón aumentó de forma dependiente a la dosis después ser tratados con 3,125-25 μg/mL de CGH. La fosfatasa ácida (ACP) en los macrófagos peritoneales de ratón mejoró significativamente después de que se estimulara con CGH en algunas concentraciones. El CGH estimuló la secreción de NO a partir de Raw264.7 de forma dependiente de la concentración e inhibió de forma significativa la activación excesiva de células inducidas con LPS en cierta medida. Después de una administración sucesiva de CGH durante 21 días, el índice esplénico de ICR aumentó significativamente a 3,73 mg/g para el grupo al cual se había administrado 800 mg/kg de CGH. El ensayo de malondialdehído (MDA) mostró que los 200 mg/kg de CGH hicieron aumentar la concentración de MDA del suero de ratón, mientras que la variación fue obsoleta en los otros grupos de CGH. Todos los resultados demostraron que CGH obtuvo efectos esenciales en las actividades inmunomoduladoras. ARTICLE HISTORY

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The immunopotentiating effects of shark-derived protein hydrolysate

Cited by 39 publications

References 49 publications

Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum Vulgare) globulin

Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum Vulgare) globulin

In vitro immuno-modulatory ability of tryptic caseinate hydrolysate affected by prior caseinate glycation using the Maillard reaction or transglutaminase

Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

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