Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

Abstract

2017)Immunomodulatory activity of small molecular (≤3 kDa) Coix glutelin enzymatic hydrolysate, CyTA -Journal of Food, 15:1, 41-48, ABSTRACT In this study, small molecular (≤3 kDa) Coix glutelin hydrolysate (CGH) was obtained by the method of enzymatic hydrolysis and then immunomodulatory activity was evaluated in vitro and in vivo. Stimulation index of mice splenocytes was increased in a dose-dependent manner after treated with 3.125-25 μg/mL CGH. Acid phosphatase in mice peritoneal macrophages was significantly improved after stimulated with CGH at some concentration. The CGH stimulated the secretion of NO from Raw264.7 in a concentration-dependent manner and also significantly inhibited lipopolysaccharideinduced cell excessive activation at some extent. After successive administration of CGH for 21 days, the spleen index of ICR was significantly increased to 3.73 mg/g for the group administrated 800 mg/kg CGH. Malondialdehyde (MDA) assay displayed that 200 mg/kg CGH increased mice serum MDA concentration, while the variation was obsolete in the other CGH groups. All results demonstrated that CGH took essential effects on immunomodulatory activities.Actividad inmunomoduladora de hidrolisato glutelina encimático de moléculas pequeñas de Coix (≤ 3 kDa) RESUMEN En este estudio, se obtuvo hidrolisato glutelina de Coix (CGH) de molécula pequeña (≤ 3 kDa) mediante el método de hidrólisis enzimática y después se evaluó la actividad sinmunomoduladora in vitro y in vivo. El índice de estimulación (SI) de los esplenocitos de ratón aumentó de forma dependiente a la dosis después ser tratados con 3,125-25 μg/mL de CGH. La fosfatasa ácida (ACP) en los macrófagos peritoneales de ratón mejoró significativamente después de que se estimulara con CGH en algunas concentraciones. El CGH estimuló la secreción de NO a partir de Raw264.7 de forma dependiente de la concentración e inhibió de forma significativa la activación excesiva de células inducidas con LPS en cierta medida. Después de una administración sucesiva de CGH durante 21 días, el índice esplénico de ICR aumentó significativamente a 3,73 mg/g para el grupo al cual se había administrado 800 mg/kg de CGH. El ensayo de malondialdehído (MDA) mostró que los 200 mg/kg de CGH hicieron aumentar la concentración de MDA del suero de ratón, mientras que la variación fue obsoleta en los otros grupos de CGH. Todos los resultados demostraron que CGH obtuvo efectos esenciales en las actividades inmunomoduladoras. ARTICLE HISTORY

Purification and Characterization of a Novel Pentadecapeptide from Protein Hydrolysates of Cyclina sinensis and Its Immunomodulatory Effects on RAW264.7 Cells

“…In addition, the protein levels of iNOS increased remarkably after SCSP treatment ( P < 0.05) (Figure 7b). These results demonstrated that SCSP up-regulated the protein levels of iNOS and NO secretion in RAW 264.7 cells in a dose-dependent manner, which is consistent with that previously demonstrated for oviductus ranae [36], yellow field pea seeds [41] and Coix glutelin protein hydrolysates (≤3 kDa) [42].…”

Identification and Molecular Mechanism of Novel Immunomodulatory Peptides from Gelatin Hydrolysates: Molecular Docking, Dynamic Simulation, and Cell Experiments

“…Food-derived immunomodulatory peptides regulated the innate immune system by binding to specific receptors on the surface of target cells. , Immunomodulatory peptides can stimulate the release of cytokines to enhance immunity or reduce the LPS-induced production of inflammatory cytokines to achieve the anti-inflammatory effect . Immunomodulatory peptides have been isolated from various foods and identified using in vitro enzymolysis via trypsin and pepsin. − In recent years, donkey-hide gelatin has attracted significant attention from researchers throughout the world because of its various activities, such as replenishing blood, anti-photoaging, immunity-enhancing, and anti-inflammation. , Donkey-hide gelatin is prepared from the skin of Equus asinus L, and it has been reported to be rich in protein (60–80% protein by weight) − Consequently, donkey-hide gelatin is considered to be an excellent source of immunomodulatory peptides. In particular, low-molecular-weight peptides (below 1000 Da) derived from donkey-hide gelatin have been demonstrated to improve the humoral immunity and cell-mediated immunity of mice…”

Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway

“…Hence, the use of low Mw enzymatic food-derived bioactive peptides to develop functional foods or nutraceutical has become an effective value addition approach for agricultural products. Previous studies have shown that protein hydrolysates derived from various marine organisms possess many bioactivities including immunomodulatory [6,7], anti-inflammatory [8,9], antioxidant [10,11], anticancer [12,13], and antimicrobial [14,15,16] effects. Some marine bioactive peptides have been approved for clinical phase assessment or introduced in the commercial market as pharmaceutical products and functional foods [17].…”