The Identification of Outer Membrane Proteins and Flagella of Campylobacter jejuni

Newell, Diane G.; McBride, H.; Pearson, Andrew D.J.

doi:10.1099/00221287-130-5-1201

Cited by 57 publications

(77 citation statements)

References 24 publications

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“…Despite the relatively high molecular weight of campylobacter flagella (Newell, McBride & Pearson, 1984), their amino acid composition, in terms of mole percentage and relative proportions of hydrophobic, acidic, basic and aromatic residues, shows a marked consistency with previously published data for other bacterial flagella (Maruyama, Lodderstaedt & Schmidt, 1978).…”

Section: Discussionsupporting

confidence: 86%

“…However, the only difference between the strains detectable by SDS-PAGE was the absence of a major 62 kDa and a minor 84 kDa protein from the outer membrane of the aflagellate variant. This 62 kDa protein has been shown to be flagellin (Newell, McBride & Pearson, 1984). The 84 kDa protein is possibly related to the 'cartwheel structure' which is found in close association with flagella and which has been shown to be absent in this aflagellate strain (SF-2) (Curry, Fox & Jones, 1984).…”

Section: Discussionmentioning

confidence: 89%

“…The selection of non-motile variants from C. jejuni has previously been described (Newell, McBride & Pearson, 1984). Briefly, non-motile organisms were enriched in the population by sequential subculture from the centre of semi-solid nutrient gelatin agar (1 % peptone, 0-33 0o yeast extract, 05 00 sodium chloride, 0O8 00 gelatin and 075 0% agar) stab cultures.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Non-specific binding was eliminated by incubation of the nitrocellulose blots in 30 bovine serum albumin in 10 mm Tris, 0900 sodium chloride, pH 7*4, for 2 h at 37 'C. Colonies of flagellate and aflagellate bacteria were differentiated by incubation for 2 h at 37 'C in 1: 100 dilution of rabbit anti-flagella antiserum (Newell, McBride & Pearson, 1984) followed by 1:1000 dilution of peroxidase conjugated goat anti-rabbit IgG antiserum (Miles Research Laboratories, Slough).…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Sarkosyl-insoluble outer membrane and flagella preparations from the wild-type strain and variants were prepared as previously described (Newell, McBride & Pearson, 1984).…”

Section: Bacterial Strainsmentioning

confidence: 99%

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Investigations on the role of flagella in the colonization of infant mice withCampylobacter jejuniand attachment ofCampylobacter jejunito human epithelial cell lines

Newell

McBride

Dolby³

1985

J. Hyg.

127

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SUMMARYThe biochemical and biological properties of the flagella of Campylobacterjejuni have been investigated using two variants selected from a flagellate, motile clinical isolate (strain 81116): a flagellate, non-motile variant (SF-1) and an aflagellate variant (SF-2). Phenotypic and biochemical analysis of the strains and amino acid analysis of the isolated flagella suggest that the variants differed from the wild-type strain only in the absence of flagella and/or motility. The aflagellate variant poorly colonized the gastrointestinal tract of infant mice but the flagellate, non-motile variant colonized the mice as successfully as the wild-type strain. 35S-labelled organisms were used to investigate the attachment of the variants to human epithelial cell monolayers in vitro. The flagellate, non-motile strain attached more efficiently to the cells than the wild-type strain or the aflagellate strain. Differences in attachment suggest that an adhesin is intimately associated with flagella of C. jejuni and that active flagella mediate only a tenuous association with host cells. This adhesin attached most efficiently to cells of intestinal epithelial origin and was not specifically inhibited by various sugars.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 89%

Section: Bacterial Strainsmentioning

confidence: 99%

Section: Bacterial Strainsmentioning

confidence: 99%

“…Sarkosyl-insoluble outer membrane and flagella preparations from the wild-type strain and variants were prepared as previously described (Newell, McBride & Pearson, 1984).…”

Section: Bacterial Strainsmentioning

confidence: 99%

See 3 more Smart Citations