Antigenic differences in congenic chicken-colonizing and noncolonizing strains ofCampylobacter jejuni

Meinersmann, Richard J.; Stern, Norman J.; Blankenship, L. C.

doi:10.1007/bf02090094

Cited by 13 publications

(9 citation statements)

References 16 publications

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“…Moreover, SF-1, displaying a motility similar to R 1 -V2, colonizes 1000-fold better than WT, and the completely nonmotile mutant R2 colonizes as efficiently as R1. The finding that motility is not required for colonization is in contrast to data from previous animal model studies (Newel1 et al, 1985;Morooka et al, 1985;Pavlovskis et a/., 1991). Possible explanations for this anomaly are the differences in the animal models and the motility mutants used.…”

Section: Discussioncontrasting

confidence: 89%

“…Possible explanations for this anomaly are the differences in the animal models and the motility mutants used. Morooka et al (1985) and Newell et al (1985) used non-defined motility mutants and variants to demonstrate the importance of motility for colonization in a suckling mouse model. Such mutants may have had concomitant changes in other colonization factors.…”

Section: Discussionmentioning

confidence: 99%

“…For Campylobacter colonization in the chicken, the factors involved in such interactions can only be speculative. However, a chicken colonization model has recently been developed (Stern et al, 1988), which may allow bacterial colonization factors to be identified (Meinersmann, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…The pathogenesis of campylobacteriosis is also largely unknown, but several virulence factors have been identified including cytotoxins (Guerrant et al, 1987;Mahajan & Rodgers, 1990), adhesins (De Melo & Pechere, 1990) and flagella (Newell et al, 1985;Morooka et al, 1985). The flagella, which confer motility upon the *Author for correspondence.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro studies have established a role for flagella in invasion (Wassenaar et al, 1991), possibly via adherence (Newell et al, 1985). Furthermore, the importance of motility in colonization has been demonstrated in several animal models, including mice (Morooka et al, 1985;Newell et al, 1985), rabbits (Caldwell et al, 1985;Pavlovskis et al, 1991) and hamsters (Aguero-Rosenfeld et al, 1990). Volunteer experiments also suggest a role for flagella in human infection (Black et al, 1988), but as yet the role in chicken colonization has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression

Wassenaar

Zeijst

Ayling³

et al. 1993

Journal of General Microbiology

225

214

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Campylobacter jejuni strain 81 116 contains two flagellin genes, flaA and JlaB. Wild-type (WT) bacteria express JlaA only, but JlaB can be expressed under certain conditions. We have determined the importance of flagella for colonization of the avian caecum, which appears to be the natural environment for these bacteria. Mutants in which JlaA orJlaB, or both had been inactivated, and motility variants, were investigated. Flagella are not a requisite for colonization, but mutants lacking both flagellin genes colonized less efficiently than WT. Inactivation of the JlaB gene, which had no effect on bacterial motility, enhanced chicken caecal colonization 1000-fold compared to WT. A variant (SF-1) with flagella composed of flagellin A, but with poor motility, also colonized better than WT. Conversely, mutants with an inactivatedJEaA gene colonized 100-to 1000-fold less efficiently than WT, regardless of their motility conferred by truncated or full-length flagellin B flagella. These results suggest that the presence of flagellin A, rather than motility, is essential for optimal bacterial colonization of chicken caeca.

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Section: Discussioncontrasting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression

Wassenaar

Zeijst

Ayling³

et al. 1993

Journal of General Microbiology

225

214

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Apoptotic Effect of Outer-Membrane Proteins from Campylobacter jejuni on Chicken Lymphocytes

et al. 1999

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Campylobacter jejuni is a significant cause of food-borne diseases in humans. The bacterium is considered a commensal organism in chickens, and it can heavily colonize chickens without causing inflammation. Poultry may be the major reservoir for the human infection in developed countries. Here we show that an outer-membrane protein extract prepared from the bacteria caused apoptosis of chicken lymphocytes detected in vitro with the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay that preferentially labels individual apoptotic cells. Blood- and spleen-lymphocytes from different-aged chickens displayed a significantly greater percentage of apoptotic cells after culture with the outer-membrane proteins from C. jejuni than controls treated with phosphate-buffered saline, chicken ovalbumin, or outer-membrane proteins prepared from E. coli strain BL21. The C. jejuni extract also produced apoptosis of chicken lymphoblastoid tumor cell lines. Apoptosis was blocked by pretreating the extract with proteinase K or antiserum against outer-membrane proteins. The results suggest that C. jejuni may be capable of achieving immune avoidance in chickens by causing apoptosis of lymphocytes.

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Cloning of an Outer Membrane Protein Gene from Campylobacter jejuni

Meinersmann

Hiett

Tarplay

1997

Current Microbiology

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An antigen in the outer membrane protein (OMP) fraction of Campylobacter jejuni was identified and characterized. Western blot analysis demonstrated antigenic differences in this protein between two congenic C. jejuni strains. Strain A74/C, which colonizes chickens, expressed the antigen at 34 kDa, while strain A74/O, which poorly colonizes chickens, expressed the antigen at 32 and 34 kDa. A genomic library was constructed in lambdagt11 with DNA from A74/O and screened with antibody raised against C. jejuni OMPs. A clone that possessed a 1.3-kb insert and expressed an immunoreactive protein fused to beta-galactosidase was isolated and purified. DNA sequence analysis revealed the insert contained one open reading frame 864 bases long. The deduced amino acid sequence demonstrated 56.3% similarity with Bacillus steorothermophilus glnH, a glutamine-binding protein, and 54.0% similarity with C. jejuni PEB1, a putative colonization adhesin. Southern hybridization, Northern hybridization, and DNA sequence analyses of the congenic colonizing and noncolonizing strains of C. jejuni failed to distinguish the two strains and revealed only one copy of the gene. Post-translational modification may be an alternate explanation for the antigenic differences seen between the two strains.

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Antigenic differences in congenic chicken-colonizing and noncolonizing strains ofCampylobacter jejuni

Cited by 13 publications

References 16 publications

Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression

Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression

Apoptotic Effect of Outer-Membrane Proteins from Campylobacter jejuni on Chicken Lymphocytes

Cloning of an Outer Membrane Protein Gene from Campylobacter jejuni

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