The <i>occhiolino (occ)</i> mutant Zebrafish, a model for development of the optical function in the biological lens

Aose, Masamoto; Linbo, Tor; Lawrence, Owen; Senoo, Tadashi; Raible, David W.; Clark, John I.

doi:10.1002/dvdy.24511

Cited by 8 publications

(12 citation statements)

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“…129 In zebrafish, the optical system is fully capable of OKR after 96 hours of development. 127 Among others, zebrafish OKR studies have revealed neuronal signaling pathways, [130][131][132][133] pharmacological and environmental impact, [134][135][136][137] and genetic players and mechanistic pathways 130,132,[138][139][140][141][142][143][144][145] involved in vertebrate vision.…”

Section: Functional Vision Testingmentioning

confidence: 99%

Zebrafish as an Animal Model for Ocular Toxicity Testing: A Review of Ocular Anatomy and Functional Assays

2020

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Xenobiotics make their way into organisms from diverse sources including diet, medication, and pollution. Our understanding of ocular toxicities from xenobiotics in humans, livestock, and wildlife is growing thanks to laboratory animal models. Anatomy and physiology are conserved among vertebrate eyes, and studies with common mammalian preclinical species (rodent, dog) can predict human ocular toxicity. However, since the eye is susceptible to toxicities that may not involve a histological correlate, and these species rely heavily on smell and hearing to navigate their world, discovering visual deficits can be challenging with traditional animal models. Alternative models capable of identifying functional impacts on vision and requiring minimal amounts of chemical are valuable assets to toxicology. Human and zebrafish eyes are anatomically and functionally similar, and it has been reported that several common human ocular toxicants cause comparable toxicity in zebrafish. Vision develops rapidly in zebrafish; the tiny larvae rely on visual cues as early as 4 days, and behavioral responses to those cues can be monitored in high-throughput fashion. This article describes the comparative anatomy of the zebrafish eye, the notable differences from the mammalian eye, and presents practical applications of this underutilized model for assessment of ocular toxicity.

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Section: Functional Vision Testingmentioning

confidence: 99%

Zebrafish as an Animal Model for Ocular Toxicity Testing: A Review of Ocular Anatomy and Functional Assays

2020

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“…Secondly, the lens is transparent, and its transparency is due to the presence in it of special crystallin proteins with a complex structure characteristic of thermal chaperones (Jester, 2008;Aose et al, 2017). Some toxicants and penetrating radiation during chronic exposure can disrupt this structure, and then opacities will appear in the fiber part of the lens (Boswell et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Amphibian and fish eye lens used as biomarker of remote and chronic environmental contamination

Simakov

Nikiforov-Nikishin

et al. 2021

Ecol. Monten.

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The lens of vertebrates has a general structural plan and reacts similarly to various factors of anthropogenic impact. Rainbow trout (Oncorhynchus mykiss) and grass frog (Rana temporaria L.) are the most well-studied objects of aquatic toxicology in Russia, which react to the level of exposure to toxicants in the urbanized environment and retain the consequences of this exposure. The study aims to determine the possibility of using the lens of fish and amphibians as a biomarker of chronic and long-term background environmental contamination. The fundamental importance of the work lies in the fact that for the first time the mechanisms of the response of the epithelial cells of the eye lens and lens fibers to the spectrum of various toxicants entering the environment with industrial effluents are considered. In chronic experiments, the sensitivity of the lens of Oncorhynchus mykiss fingerlings to three toxicants (dibutyl sebacate-DBS, polyterpenes-PTP, and 3-aminopropyltriethoxysilane-AGM-9) in concentrations close to the normalized valuesin the environment was studied. On adult individuals of Rana temporaria, the appearance of abnormities in the lens was studied over a long period, depending on the total environmental contamination. To assess remote environmental contamination, studies were carried out on the constantly growing lens of the grass frog, which can preserve the resulting fiber abnormities throughout its life in the form of local opacities from the harmful environmental factors impacts. On the epithelium of the lens of fish, it was found that under the influence of certain pollutants, the mitotic index (MI) in the germinal area can change over a wide range. With the combined effect of toxicants, inhibition of mitosis is also observed. Using biomicroscopy of the eye, it was possible to show that frogs from the urbanized zone of Moscow have 1.5 times more genotypic and modification changes in the lens than their relatives taken from an ecologically clean zone of the Moscow region, Mozhaisky district in the water area of the Ozerninskoye reservoir. For the first time, the sensitivity of the lens of aquatic organisms to chronic effects under experimental and background contamination of the terrestrial and aquatic environment has been shown. Therefore, taking into account the uniformity of the structure of the eye lens of fish and amphibians, as well as in other vertebrates, the lens can be used as a biomarker for assessing chronic and remote environmental contamination. The research results can be applied for a comprehensive assessment of the action of anthropogenic factors.

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“…20,21 The rapidly and externally developing transparent zebrafish embryos are amenable to easy genetic manipulation, allowing fast generation and identification of mutants modelling human ocular genetic disorders. [22][23][24][25][26][27][28] Such disease models can be concurrently investigated in large-scale genetics, drug screening, in vivo cell biology of early disease development as well as behavioral assays. [29][30][31][32] These potentials substantially aid fast progress in the validation of human genome association studies and in preclinical therapy development paths toward the early diagnosis and/or restoration of visual function.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the zebrafishatoh7−/−Mutant,lakritz, highlights Atoh7‐dependent genetic networks with potential implications for human eye diseases

et al. 2020

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Expression of the bHLH transcription protein Atoh7 is a crucial factor conferring competence to retinal progenitor cells for the development of retinal ganglion cells. Several studies have emerged establishing ATOH7 as a retinal disease gene. Remarkably, such studies uncovered ATOH7 variants associated with global eye defects including optic nerve hypoplasia, microphthalmia, retinal vascular disorders, and glaucoma. The complex genetic networks and cellular decisions arising downstream of atoh7 expression, and how their dysregulation cause development of such disease traits remains unknown. To begin to understand such Atoh7‐dependent events in vivo, we performed transcriptome analysis of wild‐type and atoh7 mutant ( lakritz ) zebrafish embryos at the onset of retinal ganglion cell differentiation. We investigated in silico interplays of atoh7 and other disease‐related genes and pathways. By network reconstruction analysis of differentially expressed genes, we identified gene clusters enriched in retinal development, cell cycle, chromatin remodeling, stress response, and Wnt pathways. By weighted gene coexpression network, we identified coexpression modules affected by the mutation and enriched in retina development genes tightly connected to atoh7 . We established the groundwork whereby Atoh7‐linked cellular and molecular processes can be investigated in the dynamic multi‐tissue environment of the developing normal and diseased vertebrate eye.

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The occhiolino (occ) mutant Zebrafish, a model for development of the optical function in the biological lens

Abstract: The occ Zebrafish is a novel disease model for visual defects associated with abnormal lens development. Developmental Dynamics 246:915-924, 2017. © 2017 Wiley Periodicals, Inc.

Cited by 8 publications

References 50 publications

Zebrafish as an Animal Model for Ocular Toxicity Testing: A Review of Ocular Anatomy and Functional Assays

Zebrafish as an Animal Model for Ocular Toxicity Testing: A Review of Ocular Anatomy and Functional Assays

Amphibian and fish eye lens used as biomarker of remote and chronic environmental contamination

Transcriptome analysis of the zebrafishatoh7−/−Mutant,lakritz, highlights Atoh7‐dependent genetic networks with potential implications for human eye diseases

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