The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population

Abstract

Lack of protein-coding capacity is a main characteristic of long noncoding RNAs (lncRNAs) which, as molecular biomarkers, have found a novel pharmacological application in cancer and are reported to be important regulators of gene expression. H19 is reportedly involved in cancer progression and tumorigenesis. One of the most common types of head and neck cancers is oral squamous cell carcinoma (OSCC). The main objective of the present study was to evaluate the correlation of OSCC susceptibility with H19 gene in an Iranian population. This research was performed on 400 subjects of both sexes referred to the Namazi Hospital affiliated with the Shiraz University of Medical Sciences (SUMS). Individuals aged 15-88 years were divided into two groups: pathologically diagnosed patients with new-onset OSCC and healthy controls. After written and informed consent was obtained from the individuals, genomic DNA was extracted. The tetra-primer ARMS-PCR technique was performed for DNA genotyping by the use of specific primer pairs. The susceptibility of OSCC and H19 gene polymorphism sites was further analyzed (rs217727 and rs2107425). The allele and genotype frequencies of H19 rs2107425 polymorphism were similar between OSCC cases and controls. The H19 rs217727T allele frequency was significantly higher in OSCC cases (P=0.002), and the polymorphism of H19 rs217727 was associated with OSCC susceptibility in the codominant (OR=6.04, 95%CI=1.70−21.42, P=0.001 for TT genotype), dominant (OR=1.62, 95%CI=1.08−2.43, P=0.01), and recessive (OR=5.32, 95%CI=1.51−18.69, P=0.003) models. This study showed that rs217727 and OSCC susceptibility were statistically correlated in the Iranian population.

Long non-coding RNA H19 expression and functional polymorphism rs217727 are linked to increased ischemic stroke risk

“…To evaluate the purity and concentration of the isolated DNA, a photo nanometer was applied at 230, 260, and 280 nm ed. Polymorphism has been evaluated by a tetra-amplification refractory mutation systempolymerase chain reaction (T-ARMS-PCR) as described previously by Ghapanchi et al (2020) [24]. Using the Tera-ARMS PCR method in genomic research allows for rapid SNP identification in a reliable (accuracy of 99.9%), and low-cost manner [28].…”

“…Previous studies suggested that rs217727 polymorphism is related to the pathogenesis of breast cancer, oral squamous cell carcinoma, and type 2 diabetes susceptibility in the Iranian population [19,24,25]. Research on the role of lncRNAs in IS pathogenesis is just beginning, and we have performed this study to assess the association between lncRNA H19 expression and its rs217727 polymorphism with susceptibility to IS in the Iranian population.…”

Long Non-Coding RNA H19 Expression and Functional Polymorphism rs217727 Are Linked to Increased Ischemic Stroke Risk

“…To evaluate the purity and concentration of the isolated DNA, a photo nanometer was applied at 230, 260, and 280 nm ed. Polymorphism has been evaluated by a tetra-ampli cation refractory mutation systempolymerase chain reaction (T-ARMS-PCR) as described previously by [30]. Using the Tera-ARMS PCR method in genomic research allows for rapid SNP identi cation in a reliable (accuracy of 99.9%), and low-cost manner [31].…”

“…Previous studies suggested that rs217727 polymorphism is related to the pathogenesis of breast cancer, oral squamous cell carcinoma, and type 2 diabetes susceptibility in the Iranian population [21,26,27]. Research on the role of lncRNAs in IS pathogenesis is just beginning, and we have performed this study to assess the association between lncRNA H19 expression and its rs217727 polymorphism with susceptibility to IS in the Iranian population.…”

An emerging potential therapeutic target for osteoporosis: LncRNA H19/miR-29a-3p axis

“…LncRNA H19 rs217727 polymorphism has been found to be associated with oral squamous cell carcinoma susceptibility in Iranian population. 10 LncRNA H19 has also been found to promote tumorigenesis of multiple myeloma by activating BRD4 signaling by targeting miR-152-3p. 11 Moreover, LncRNA H19 has been validated as a novel therapeutic target for pancreatic cancer.…”