The high incidence of meiotic errors increases with decreased sperm count in severe male factor infertilities

Abstract

BACKGROUND:The high frequency of aneuploidy sperm raises concerns that there may be an increased incidence of aneuploid offspring in ICSI programmes. In order to assess the role that chromosome complement plays in normal and abnormal fertility, detailed molecular cytogenetic studies must be done on sperm samples from men with normal and abnormal fertility. METHODS: To understand more clearly the cytogenetic make-up of sperm from oligoasthenoteratozoospermic (OAT) patients, multi-colour fluorescence in situ hybridization was used to determine numerical chromosome abnormalities. RESULTS: Increased aneuploidy frequencies for chromosomes 13, 18, 21, X and Y were detected in sperm from OAT patients. The frequencies of diploidy also increased. There were no differences in non-disjunction at meiosis I compared to meiosis II. Sperm count inversely correlated with the frequencies of diploidy, aneuploidies for chromosomes 13 and 21 in OAT patients. Twenty-two cycles of ICSI and 18 embryo transfers were performed in 20 couples. Only three cases achieved successful pregnancies. CONCLUSIONS: A higher incidence of meiotic errors and lower sperm counts was found in sperm from OAT patients.

Clinical and prognostic importance of chromosomal abnormalities, Y chromosome microdeletions, and CFTR gene mutations in individuals with azoospermia or severe oligospermia

“…The lack of data regarding sperm aneuploidy rates is another limitation of our study. Several studies on the association of Y chromosome AZF microdeletion with male infertility detected microdeletions in 1% to 55% of infertile men with azoospermia or severe oligospermia (14)(15)(16). This large variation among reports may be attributable to ethnic differences or to the criteria applied for patient selection.…”

Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

“…Although swim-up and Percoll fractionation techniques generally increase the quality of semen samples used in IVF [25], a high proportion of attempts still fail to achieve a successful outcome. Given that SFC offers an aneuploidy screening of all chromosomes, while FISH technique analyses specific chromosomes overlooking a significant amount of aneuploidies [14,49], as well as that SFC is a less time and money consuming method compared to FISH, we could suggest SFC for a better evaluation of sperm quality. This technique would help us discriminate which ART technique, conventional IVF or ICSI, is appropriate so as to increase the possibility of a successful outcome, even when sperm concentration and motility are within normal range according to WHO criteria.…”

“…The aneuploidy of these gametes may be ascribed to an altered intratesticular environment that disrupts the chromosome segregation mechanisms [13]. Many studies have reported an inverse correlation between sperm aneuploidy rates and conventional semen parameters [14][15][16][17][18][19], confirming the association of chromosomal abnormalities with poor sperm quality.…”

Comparison of Aneuploidy Frequency to Sperm FISH and Sperm Apoptosis Results in Embryos That Lost the Vitality

“…yap tık la rı ça lış ma da IC SI uy gu la nan ol gu lar da da ha yük sek oran da sperm FISH ano ma li ora nı tespit et miş ler dir. 48 Mar tin ve ark. oli go zo os per mik ol gu lar da kro mo zom sa yı ano ma li le ri için art mış risk ol du ğu nu gös ter miş ler dir.…”