Comparison of Aneuploidy Frequency to Sperm FISH and Sperm Apoptosis Results in Embryos That Lost the Vitality

Çankaya, Tufan; Ozkinay, Cihangir; Gündüz, Cumhur; Tavmergen, Erol; Bora, Elçin; Özkınay, Ferda

doi:10.5336/medsci.2010-16982

Cited by 3 publications

(3 citation statements)

References 44 publications

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“…Curiously, in contrast to published work showing the association of chromosome 21 disomy to infertile male semen (Colombero et al, ; Templado et al, ), we did not observe an effect of SGD on embryonic chromosome 21 trisomy in the AMA‐SGD group; however, the paternal contribution may be masked by the high maternal contribution to trisomy 21 reported by others (Egozcue et al, ; Munné et al, ; Gianaroli et al, ; Hassold et al, ; Coco, ). Finally, embryo aneuploidies in chromosomes 13 and 18 were significantly higher in AMA‐SGD than Y‐SGD (Fig ), supporting the prediction that aged oocytes cannot repair SGDs linked to these two chromosomes (Çankaya et al, ).…”

Section: Discussionsupporting

confidence: 80%

“…). This result is consistent with the high frequency of diploidy in sperm of infertile patients (Egozcue et al, ; Bernardini et al, ; Munné and Cohen, ; Pang et al, ; Ushijima et al, ; Shi et al, ; Zhu et al, ), which can lead to embryonic polyploidy (Benkhalifa et al, ; Kahraman et al, ; Çankaya et al, ). We also observed elevated nullisomy in the AMA‐SGD embryos compared to AMA‐N embryos (19% vs. 6%) (Fig.…”

Section: Discussionsupporting

confidence: 78%

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Impact of sperm genome decay on Day‐3 embryo chromosomal abnormalities from advanced‐maternal‐age patients

Kaarouch

Bouamoud

Louanjli³

et al. 2015

Molecular Reproduction Devel

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Infertile male patients often exhibit unconventional semen parameters, including DNA fragmentation, chromatin dispersion, and aneuploidy-collectively referred to as sperm genome decay (SGD). We investigated the correlation of SGD to embryo chromosomal abnormalities and its effect on clinical pregnancy rates in patients with advanced maternal age (AMA) (>40 years) who were undergoing intracytoplasmic sperm injection-preimplantation genetic screening (ICSI-PGS). Three groups were assessed: patients with AMA and male partners with normal sperm (AMA-N); AMA patients and male partners presenting with SGD (AMA-SGD); and young fertile female patients and male partners with SGD (Y-SGD). We found a significant increase in embryonic chromosomal abnormalities-polyploidy, nullisomy, mosaicism, and chaotic anomaly rates-when semen parameters are altered (76% vs. 67% and 66% in AMA-SGD vs. AMA-N and Y-SGD groups, respectively). Statistical analysis showed a correlation between SGD and aneuploidies of embryonic chromosomes 13, 16, 21, X, and Y, as well as negative clinical outcomes. Incorporation of molecular sperm analyses should therefore significantly minimize the risk of transmission of chromosomal anomalies from spermatozoa to embryos, and may provide better predictors of pregnancy than conventional sperm analyses. We also demonstrated that an ICSI-PGS program should be implemented for SGD patients in order to limit transmission of chromosomal paternal anomalies and to improve clinical outcome.

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