The heparin-binding protein interactome in pancreatic diseases

Nunes, Quentin; Mournetas, Virginie; Lane, Brian; Fernig, David G.; Vasieva, Olga

doi:10.1016/j.pan.2013.08.004

Cited by 16 publications

(17 citation statements)

References 45 publications

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“…All of these clearly underlie periodontitis and highlight the likely importance of HBPs collectively in the disease. HBPs, by virtue of their extracellular location and key functions in cell communication are readily accessible significant therapeutic targets [ 3 – 5 ]. Therefore, we sought to identify potential drug targets within the putative HBP interactome.…”

Section: Resultsmentioning

confidence: 99%

“…A PubMed search identified 249 genes that were up regulated and 146 genes that were down regulated in periodontal disease, compared with periodontal disease-free gingival samples (supplementary information, [ 4 ]). Interactions between HBPs in periodontitis were obtained using the online database resource ‘Search Tool for the Retrieval of Interacting Genes’ (STRING), as described previously for analogous datasets of HBPs in normal pancreas and pancreatic disease [ 5 ]. STRING 9.1 is a database of known and predicted functional interactions and is a comprehensive resource that can be used with Cytoscape [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…Tools for gene ontology (GO) term enrichment were used to undertake functional analyses of the HBPs in periodontitis, as described previously [ 5 ]. GO covers biological process (BP), cellular component (CC) and molecular function (MF) sub-ontologies.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore identifying such interactions will be useful in determining the target towards therapeutic development. From this angle, heparin-binding proteins (HBPs) which are extracellular regulatory proteins that mediate cell communication in development, homeostasis and disease [ 3 – 5 ] appear to be very important in the understanding of the progression of periodontitis. It has been reported that HBPs such as azurocidin could be a potential candidate for a biomarker for the early detection of inflammatory periodontal destruction [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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In silico analyses of heparin binding proteins expression in human periodontal tissues

et al. 2016

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BackgroundPeriodontitis is described as a group of inflammatory diseases of the gingiva and supporting structures of the periodontium. The accumulation of plaque bacteria, which include putative periodontal pathogens, is known to initiate the disease but the host immune response is the major contributing factor for destruction of periodontal tissues. Proteins that bind to heparin heparin-binding protein (HBPs) play important roles in health and disease and interact with each other via networks known as ‘heparin interactomes’. This study aimed at evaluating published datasets of HBPs and its role in periodontitis.MethodsTo elucidate the role of HBPs in periodontitis, bioinformatics analyses of published data was used. In silico analyses of published datasets were used to construct a putative HBPs interactome using an online database resource, ‘STRING’ (Search Tool for the Retrieval of Interacting Genes).ResultsPubMed searches identified 249 genes that were up regulated and 146 genes that were down regulated in periodontal disease, compared with periodontal disease-free gingival samples. In silico analyses using published datasets revealed 25 up-regulated and 23 down-regulated HBPs in periodontitis. Of these HBPs; chemokines, such as CXCL12 was up regulated where as some of the matrixmetalloproteinases (MMPs; MMP-2 and MMP9) were up-regulated while MMP-14 was down regulated.ConclusionsThe results indicate that HBP analyses will provide multiple targets for the biological mechanisms underlying periodontal disease (such as MMPs, cytokines and chemokines) that will have important clinical implications in the future drug design and management of periodontal disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-016-1857-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In silico analyses of heparin binding proteins expression in human periodontal tissues

et al. 2016

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“…14 Furthermore, HS has been implicated in diseases such as Alzheimer's, 15 cancer 16 and sexually transmitted infections. 17 Recently, Nunes et al 18 performed a study to examine the role of HEPbps in pancreatic diseases, concluding that a concerted network of highly connected HEPbps was important for distinguishing between normal and diseased pancreatic tissue. Chen et al 19 showed that the interaction between the cell surface HSPGs of two-breast cancer cell lines and their innate complement of HEPbps is a key component of tumourigenicity.…”

Section: Introductionmentioning

confidence: 99%