The Hemalog D White Cell Differential System

Mansberg, H. P.; Saunders, Alex M.; Groner, W.

doi:10.1177/22.7.711

Cited by 127 publications

(30 citation statements)

References 5 publications

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“…There has been an especial improvement in the precision of the differential count, mainly due to the fact that the analyser screens about 10000 leukocytes in each analysis (1)(2)(3)(4).…”

mentioning

confidence: 99%

Short-Term and Long-Term Intra-Individual Variations and Critical Differences of Haematological Laboratory Parameters

Costongs¹,

Janson²,

Bas³

et al. 1985

Clinical Chemistry and Laboratory Medicine

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Summary:The complete blood cell count and the differential leukocyte count have been studied for their variations within-one-day and during one weck (short-term variations) and during a period of six months (long-term variations) with an automatic haematology analyser (H-6000, Technicon®), in which some new Parameters (large unstained cells, high peroxidase cells, red cell distribution width, platelet distribution width, mean platelet volume, plateletcrit) are included. The influence of external factors such äs sex-differences, smoking/non-smoking, üse of oral contraceptives etc. have also been studied. It appears that the use of critical differences together with haematological data improves the value of these parameters in diagnosis and treatment of patients. Laboratoriumskenngrößen. Kurz-und langzeitige intra-individuelle Änderungen sowie kritische Differenzen hämatologischer

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“…There has been an especial improvement in the precision of the differential count, mainly due to the fact that the analyser screens about 10000 leukocytes in each analysis (1)(2)(3)(4).…”

mentioning

confidence: 99%

Short-Term and Long-Term Intra-Individual Variations and Critical Differences of Haematological Laboratory Parameters

Costongs¹,

Janson²,

Bas³

et al. 1985

Clinical Chemistry and Laboratory Medicine

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“…Flow cytometric differential blood cell counting is based on various principles, including cytochemical stains (9,15), fluorescent stains such as acridine orange (1,2,18), and differences in electronic cell volume (19) or in orthogonal light scattering (7,8). Differences in P12 uptake, demonstrated in the present report, provide a potential additional parameter for differential cell counting.…”

Section: Resultsmentioning

confidence: 69%

Flow cytofluorometric analysis of the uptake of the fluorescent fatty acid pyrene‐dodecanoic acid by human peripheral blood cells

et al. 1988

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The fluorescence activated cell sorter (FACS) was used for measuring the uptake of the fluorescent fatty acid derivative U-(l-pyrene) dodecanoic acid (Pa) by human peripheral blood cells. The results indicate that blood cells differ widely in their ability to take up Pl2, with polymorphonuclear cells showing the greatest uptake, followed by lymphocytes, platelets, and RBCs. These differences in Pl2 uptake provide a potential additional parameter for differential cell counting. Using the ability of the FACS to "gate out" nonrelevant cells, it was possible to measure the rate of Pl2 uptake by each respective cell type even when admixed with other cells. Thus elaborate physical separation procedures could be avoided, and contaminating cells did not influence the results. Differences in Pl2 uptake were also utilized to separate blood cells into pure subpopulations of specific cell types. Key terms: Fluorescence activated cell sorter, fatty acid derivativesA major obstacle in studying biological and biochemical properties of specific cells is the fact that, in vivo, cells are admixed with a variety of other cell types. For example, the study of hemopoietic cells is limited by the heterogeneity of a population consisting of cells belonging to several lineages that are at various stages of maturation. Analysis of such cells requires complete separation into discrete, homogenous subpopulations, since even a small contamination by cells that differ considerably in the property examined from the subpopulation studied may affect the results.We have demonstrated that the fluorescent, mediumchain fatty acid lZ-(l-pyrene) dodecanoic acid (P12) is effectively transported across the membranes of several cultured cell types and incorporated into their neutrallipids and phospholipids (10,ll). Using in vitro established leukemic cell lines, we have shown that cells of different hemopoietic lineages and stages of maturation differ in the rate and extent of PI2 uptake as well as in their metabolism of this acid (43). Recently, we have shown that the cellular uptake of P12 can be measured by the fluorescence activated cell sorter (FACS) (13,141, The present study describes the uptake of P12 by various cell types present in human peripheral blood and shows that blood cells differ widely in their ability to take up the compound. By taking advantage of the abilpossible to measure the rate of P12 uptake by each respective cell type, even when admixed with other cells. Thus elaborate separation procedures were avoided, and contaminating cells did not influence the results. Differences in P12-uptake were also utilized to separate blood cells into pure subpopulations of specific cell types. MATERIALS AND METHODS CellsPeripheral blood from normal individuals cells was collected in preservative-free heparin or citrate. Buffy coat cells were separated by mixing 2 volumes of blood with 1 volume of 1% dextran in saline (Pharmacia, Uppsala, Sweden) and allowing it to sediment at unit gravity for about 1 hour at room temperature. The cellcontai...

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“…The rms noise level of the model 52G argon laser was also within its specified value (0.5%), but the noncompensated output light intensity fluctuations increased the ALL measurement cv considerably. Argon and krypton lasers have not been fully utilized as illumination sources in ALL measurements, compared to the small HeNe laser (5,6,7) and tungsten lamp (8). This new technique now permits axial light loss or extinction measurements on cells and particles using illumination sources operating at different wavelengths that were heretofore unusable.…”

Section: Discussionmentioning

confidence: 99%

A differential amplifier circuit for reducing noise in axial light loss measurements

Steinkamp

1983

Cytometry

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Simplified electronics have been developed for reducing laser excitation source light intensity fluctuations in flow cytometric axial light loss (extinction) measurements. By continuously monitoring the laser output with a photodiode detector and combining it with the axial light loss signal from a second detector using a high-speed differential amplifier, background interference is reduced 10 to 40 dB. Oscilloscope waveforms and frequency distribution histograms recorded from uniform-size polystyrene latex spheres and human mononuclear blood cells are used to illustrate the noise reduction capabilities.

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The Hemalog D White Cell Differential System

Cited by 127 publications

References 5 publications

Short-Term and Long-Term Intra-Individual Variations and Critical Differences of Haematological Laboratory Parameters

Short-Term and Long-Term Intra-Individual Variations and Critical Differences of Haematological Laboratory Parameters

Flow cytofluorometric analysis of the uptake of the fluorescent fatty acid pyrene‐dodecanoic acid by human peripheral blood cells

A differential amplifier circuit for reducing noise in axial light loss measurements

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