The Growth Improvement of Grammatophyllum scriptum (Lindl.) Bl. In Vitro Plantlet using Photoautotrophic Micropropagation System

Sasongko, Aries Bagus; Fatumi, Asruwaidah; Indrianto, Ari

doi:10.22146/ijbiotech.27167

Cited by 5 publications

(4 citation statements)

References 5 publications

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“…The available reports are few. They indicate variation in total chlorophyll in the range of several pg to 2 mg per g of the fresh weight of leaves (Huh et al 2017;Sasongko et al 2017). Our results are in agreement with previous studies showing that chlorophyll concentration on a unit mass basis was higher for in vitro plantlets compared to that in greenhouse plants (Premkumar et al 2001).…”

Section: Quantification Of Leaf Pigments and Phenolic Compoundssupporting

confidence: 92%

Leaf pigments and concentrations of phenolic compound in Begonia grandis plantlets obtained from the floral explants

Karpova

Nabieva

Fershalova

2021

Rend. Fis. Acc. Lincei

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The objective of this study was to design a protocol of the successful establishment of plants of Begonia grandis dry. in culture in vitro, and to evaluate the concentrations of chlorophyll a and b, carotenoids, anthocyanins and phenolic compounds, as well as the ratios of chlorophyll a/b, total chlorophyll/carotenoids and total chlorophyll/anthocyanins in the in vitro plantlets in comparison to the same parameters assessed in the leaves of greenhouse plants. A significant increase in the percentage of shoot formation (22.0 ± 1.5%) was recorded on the modified N6 medium supplemented with plant growth regulators in comparison with the standard breeding technology. Begonia grandis plantlets possessed the elevated contents of all photosynthetic pigments per unit mass of plant compared to the greenhouse plants. The increase in chlorophyll b was most substantial. The average anthocyanin concentrations in the plantlets and greenhouse plants were approximately equal, but the essential variation within B. grandis microclones was observed. Phenolic profiles of in vitro plantlets and greenhouse plants was rather similar. Concentrations per unit mass for the majority of components (especially organic and phenolic acids) in the plantlets increased in comparison to those in greenhouse plants (47.23 and 38.22 mg g −1 total phenolics, respectively). Overall data suggest that this protocol can be used for the production of a large number of plants of B. grandis in a good physiological state and with high phenolic content.

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Section: Quantification Of Leaf Pigments and Phenolic Compoundssupporting

confidence: 92%

Leaf pigments and concentrations of phenolic compound in Begonia grandis plantlets obtained from the floral explants

Karpova

Nabieva

Fershalova

2021

Rend. Fis. Acc. Lincei

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“…El efecto negativo marcado debido a la ausencia de sacarosa en el medio de cultivo registró diferencias significativas cuando se empleó 150 μmol/m -2 s -1 afectando la respuesta morfológica de los explantes, por el contrario, el empleo de intensidades de la luz de 50 y 100 μmol/m -2 s -1 y en ausencia de sacarosa fueron registrados valores similares de respuesta morfológica al emplear 30 g/L de sacarosa en el medio de cultivo, siendo la prueba no significativa (Figura 1a-1d). El número y longitud de raíces en intensidades de la luz 50 y 100 μmol/m -2 s -1 en ausencia de sacarosa, la respuesta morfológica fue similar con aquellos medios de cultivo donde se empleó sacarosa, esta respuesta es importante cuando las plántulas desarrollan bajo condiciones fotoautotróficas, porque permite mejorar el estatus de agua del brote y por consiguiente una mejor absorción de los nutrientes a través de la raíz (Sasongko et al, 2016).…”

Section: Parámetrounclassified

Intensidad de la luz y sacarosa en la respuesta morfo-fisiológica in vitro de plántulas Selenicereus megalanthus (Haw.)

Millones Chanamé,

Vásquez Castro

2023

Rev. Investig. Altoandin.

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Mejorar la eficiencia en las condiciones de cultivo in vitro en protocolos de la micropropagación de pitahaya amarilla establecidos, permite evaluar la dependencia de la sacarosa en el medio de cultivo bajo condiciones fotoautotróficas con la finalidad de obtener plántulas de alta calidad en menor tiempo, que favorezca su aclimatación en la producción masiva de esta especie. La presente investigación tuvo como objetivo evaluar los niveles de intensidad de la luz y el empleo o no de la sacarosa en el medio de cultivo sobre la respuesta morfológica y fisiológica in vitro de plántulas de pitahaya amarilla (S. megalanthus). Secciones vegetativas de pitahaya amarilla fueron colocadas en medios de cultivo de crecimiento y desarrollo para la obtención de esquejes de aproximadamente seis centímetros de largo para la obtención de los explantes. Los recipientes fueron colocados a diferentes intensidades de la luz: 50, 100 y 150 μmol/m-2s-1, utilizando lámparas fluorescentes compactas, cuyas intensidades fueron ajustadas empleando un luxómetro. Se empleó un diseño completamente al azar con arreglo factorial (Factor A: tres intensidades de luz y Factor B: presencia y ausencia de sacarosa en el medio de cultivo de crecimiento y desarrollo) y siete repeticiones por tratamiento. Las evaluaciones de la respuesta morfológica y fisiológica fueron realizadas a los 45 días de cultivo in vitro. Los rasgos relacionados con el contenido hídrico, peso seco y longitud de brote permiten explicar la respuesta fotoautotrófica de las plántulas de pitahaya amarilla cultivada in vitro empleando intensidades de luz de 50 y 100 μmol/m-2s-1.

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“…Orchid propagation with in vitro culture has been widely practiced both in the world and in Indonesia, such as Dendrobium nanum [11], Phalaenopsis amabilis [12], Dendrobium phalaenopsis [13], and Grammatophyllum scriptum [14]. It is expected that seedlings of V. tricolor 'Queen Maxima' can grow well both in vitro and ex vitro.…”

Section: Introductionmentioning

confidence: 99%

In vitroculture and characterization of theHSP70gene onVanda tricolorLindley var.Suavis‘Queen Maxima’

et al. 2020

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Vanda tricolor Lindey var. Suavis is an endemic orchid from Mt.Merapi, Yogyakarta Special Region, Indonesia. This orchid has beautiful flowers with unique patterns of white and purple spots, fragrant and can live in the slopes of Mt.Merapi which is a very active volcano. UGM in collaboration with the Netherlands carried out ex situ conservation of the V. tricolor Merapi through the self-pollination of orchids by the Queen of the Netherlands, Queen Maxima on March 11, 2020 at the UGM campus, Yogyakarta. In honor, the new generation of crosses is named Vanda tricolor var Suavis ‘Queen Maxima’. This study aims to produce mass quantities of the orchid and characterize the HSP70 gene in it. Methods: Standard in vitro culture for Vanda on MS, NP and VW medium, isolation of plant gDNA, PCR with V. tricolor HSP70primers and sequencing of amplicon DNA. The results showed that > 90 % of V. tricolor ‘Queen Maxima’ seeds germinated well in all media and the best on VW medium. V. tricolor has the HSP70 protein with PTZ00009 super family amino acid motif that 87 % similar to the HSP70 protein from the Phalaenopsis equestris orchid, which is probably the reason V. tricolor become superior to high temperature environments.

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The Growth Improvement of Grammatophyllum scriptum (Lindl.) Bl. In Vitro Plantlet using Photoautotrophic Micropropagation System

Cited by 5 publications

References 5 publications

Leaf pigments and concentrations of phenolic compound in Begonia grandis plantlets obtained from the floral explants

Leaf pigments and concentrations of phenolic compound in Begonia grandis plantlets obtained from the floral explants

Intensidad de la luz y sacarosa en la respuesta morfo-fisiológica in vitro de plántulas Selenicereus megalanthus (Haw.)

In vitroculture and characterization of theHSP70gene onVanda tricolorLindley var.Suavis‘Queen Maxima’

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