In vitroculture and characterization of theHSP70gene onVanda tricolorLindley var.Suavis‘Queen Maxima’

Abstract

Vanda tricolor Lindey var. Suavis is an endemic orchid from Mt.Merapi, Yogyakarta Special Region, Indonesia. This orchid has beautiful flowers with unique patterns of white and purple spots, fragrant and can live in the slopes of Mt.Merapi which is a very active volcano. UGM in collaboration with the Netherlands carried out ex situ conservation of the V. tricolor Merapi through the self-pollination of orchids by the Queen of the Netherlands, Queen Maxima on March 11, 2020 at the UGM campus, Yogyakarta. In honor, the new generation of crosses is named Vanda tricolor var Suavis ‘Queen Maxima’. This study aims to produce mass quantities of the orchid and characterize the HSP70 gene in it. Methods: Standard in vitro culture for Vanda on MS, NP and VW medium, isolation of plant gDNA, PCR with V. tricolor HSP70primers and sequencing of amplicon DNA. The results showed that > 90 % of V. tricolor ‘Queen Maxima’ seeds germinated well in all media and the best on VW medium. V. tricolor has the HSP70 protein with PTZ00009 super family amino acid motif that 87 % similar to the HSP70 protein from the Phalaenopsis equestris orchid, which is probably the reason V. tricolor become superior to high temperature environments.

In vitro culture of wild Vanda tricolor Lindley seeds from Kintamani, Bali, as initial step for conservation

“…Highest germination rate of Vanda tricolor Lindley var. Suavis 'Queen Maxima' was showed in VW medium [13]. Both MS and VW media are expensive therefore modified medium from 1255 (2023) 012026 IOP Publishing doi:10.1088/1755-1315/1255/1/012026 2 natural organic extract and foliar fertilizer can be an alternative option.…”