The genotoxicity of industrial wastes and effluents

Houk, Virginia S.

doi:10.1016/0165-1110(92)90001-p

Cited by 289 publications

(157 citation statements)

References 107 publications

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“…Bioassays with prokaryotes enable the detection of agents that induce gene mutation and primary DNA damages. On the other hand, analyses with eukaryotes enable the detection of a greater damage extent, varying from gene mutations to chromosome damages and aneuploidies (Houk, 1992;Leme & Marin-Morales, 2009). Using both pro-and eukaryotic test system make the results both strengthen and correlate to verify if the chemical(s) has/have really any bad effects on the genes.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Liman¹,

Gökçe²,

Akyıl³

et al. 2012

Rev. bras. farmacogn.

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Genotoxic and mutagenic effects of aqueous extract from aerial parts Linaria genistifolia (L.) Mill. subsp. genistifolia, Plantaginaceae (Lg-ext) were investigated by using both Allium cepa root meristematic cells and bacterial reverse mutation assay in Salmonella typhimurium TA98 and TA100 with or without metabolic activation system (S9), respectively. In Allium root growth inhibition test, EC50 value was determined approximately 15 g/L and 0.5xEC50, EC50 and 2xEC50 concentrations of Lg-ext were introduced to onion tuber roots and distilled water and methyl methane sulfonate (MMS, 10 ppm) used as a negative and positive control, respectively. The characteristic effect caused by tested preparations was an increase of mitotic index (MI) in 7.5 g/L and 15 g/L (except 24 and 96 h) and simultaneous decrease of MI in 30 g/L and in MMS. While stickiness, bridges, chromosome laggards and disturbed anaphase-telophase were observed in anaphase-telophase cells, c-metaphase, pro-metaphase, polyploidy and binuclear cells were observed in other cells. Lg-ext was not found to be mutagenic on S. typhimurium TA 98 and TA100 with or without S9. The results were also analyzed statistically by using SPSS for Windows, and Duncan's multiple range tests were performed respectively. These results indicate that Lg-ext exhibits genotoxic activity in A. cepa root meristematic cells but not mutagenic activity in Ames test system.

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Section: Resultsmentioning

confidence: 99%

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Liman¹,

Gökçe²,

Akyıl³

et al. 2012

Rev. bras. farmacogn.

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“…Metabolization may provide even more toxic characteristics to the analyzed samples, probably due to the high activity of metabolites generated by available substances, which led, in some cases the absence of genotoxiciy. Besides, the high toxicity presented by these samples might point out to a possible contamination by biologically active substances, such as antibiotics and disinfectants (Saxena, 1984;Houk, 1992;Vargas et al, 1993;Giuliani et al, 1996;White et al, 1996). These substances are widely used at the HCPA, and their ultimate destination is the effluents.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity and genotoxicity of untreated hospital effluents

Ortolan

Ayub

2007

Braz. arch. biol. technol.

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“…Of all the tester strains, those containing the hisD3052 allele (especially strains TA1538 and TA98) have been shown to be the most useful in mutagenesis screening programs (15) and have been used more than any of the other strains for evaluating complex mixtures (2,11). Thus, nearly all of our initial work has focused on the analysis of mutations that revert this allele.…”

Section: Molecular Methodsmentioning

confidence: 99%

“…Although other genotoxicity tests in addition to the Salmonella assay have been used to evaluate complex mixtures (6,7), the vast majority of the literature on the genotoxicity of complex mixtures has been generated using the Salmonella assay (2,6,7,11,12). Recent developments in genome analysis now provide the means by which mutations can be examined at the molecular level (13).…”

mentioning

confidence: 99%

Molecular analysis of mutations induced at the hisD3052 allele of Salmonella by single chemicals and complex mixtures.

DeMarini

Bell

Levine

et al. 1993

Environ Health Perspect

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More single chemicals and complex environmental mixtures have been evaluated for mutagenicity at the hisD3052 allele of Salmonella, primarily in strain TA98, than in any other mutation assay. The development of colony probe hybridization procedures and the application of the polymerase chain reaction and direct DNA sequencing has permitted rapid molecular access to this allele. We discuss these techniques and the resulting mutation spectra that have been induced by a variety of environmental mutagens and complex mixtures. A common GC or CG deletion within a hot-spot region of the sequence dominates most of the spectra. In addition to this two-base deletion, we have recovered about 200 other types of mutations within the 72-base target for reversion of the hisD3052 allele. These include a variety of deletions (as large as 35 bases), duplications (as large as 46 bases), and complex mutations involving base substitutions. The quasipalindromic nature of the target sequence and its potential to form DNA secondary structures and slippage mismatches appear to be an important basis for the mutability of this allele.

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The genotoxicity of industrial wastes and effluents

Cited by 289 publications

References 107 publications

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Cytotoxicity and genotoxicity of untreated hospital effluents

Molecular analysis of mutations induced at the hisD3052 allele of Salmonella by single chemicals and complex mixtures.

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