The Genetic Relationship Between Some Strawberry Cultivars and F1 Population Derived From These Cultivars

Abstract

Abstract. In this study, while Osmanli strawberry, which has some superior characteristics like taste, aroma and male sterility that makes breeding studies easier, has been used as maternal parent, three local (Karacilek, Tuylu and Deli) cultivars in Turkey and three standard (Kabarla, Sweet Ann and Sweet Charlie) strawberry cultivars were used as paternal parents. Molecular analyses were conducted for determination of polymorphism levels by using 14 UBC-ISSR primers for the examination of 52 F1 genotypes that included selections as a result of hybridization studies, parents that were used in hybridization studies, and Fragaria chiloensis. 76 DNA bands were obtained in total and 60 of them were determined as polymorphic. The resulting data of the evaluation of ISSR-PCR bands, were analysed in Popgene32 and MEGA5.0 packaged software and the dendrogram was obtained according to the UPGMA method. The dendrogram was separated into two major groups at the 20% difference level, one is larger and the other is smaller. While Fragaria chiloensis, Tuylu-2 local cultivar, CC48 and CA97 F1 genotypes participated in the small group, the other F1 genotypes and parents participated in the larger group.