The genetic diversity and structure of 18 sheep breeds exposed to isolation and selection

Ćurković, Matija; Ramljak, Jelena; Ivanković, Stanko; Mioč, Boro; Ivanković, Ante; Pavić, Vesna; Brka, Muhamed; Veit-Kensch, Claudia E.; Međugorac, Ivica

doi:10.1111/jbg.12160

Cited by 14 publications

(8 citation statements)

References 18 publications

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“…More specifically, Suffolk and Dorset animals clustered closer to other US groups than to Suffolk from Australia and Ireland, or Dorset from Australia or the UK. This observation may be partially attributed to the differences in selection and breeding strategies and in production systems across the world (Andersson ; Ćurković et al ; Wang et al ). In addition, the difference between terminal breeds and wool breeds is clear, suggesting that there are genetic differences between breeds that have been selected for alternative production objectives and purposes (Blackburn et al ; Zhang et al ; Fariello et al ).…”

Section: Discussionmentioning

confidence: 99%

Genetic structure and admixture in sheep from terminal breeds in the United States

Davenport

Hiemke²,

McKay

et al. 2020

Animal Genetics

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Summary Selection for performance in diverse production settings has resulted in variation across sheep breeds worldwide. Although sheep are an important species to the United States, the current genetic relationship among many terminal sire breeds is not well characterized. Suffolk, Hampshire, Shropshire and Oxford (terminal) and Rambouillet (dual purpose) sheep (n = 248) sampled from different flocks were genotyped using the Applied Biosystems Axiom Ovine Genotyping Array (50K), and additional Shropshire sheep (n = 26) using the Illumina Ovine SNP50 BeadChip. Relationships were investigated by calculating observed heterozygosity, inbreeding coefficients, eigenvalues, pairwise Wright’s FST estimates and an identity by state matrix. The mean observed heterozygosity for each breed ranged from 0.30 to 0.35 and was consistent with data reported in other US and Australian sheep. Suffolk from two different regions of the United States (Midwest and West) clustered separately in eigenvalue plots and the rectangular cladogram. Further, divergence was detected between Suffolk from different regions with Wright’s FST estimate. Shropshire animals showed the greatest divergence from other terminal breeds in this study. Admixture between breeds was examined using admixture, and based on cross‐validation estimates, the best fit number of populations (clusters) was K = 6. The greatest admixture was observed within Hampshire, Suffolk, and Shropshire breeds. When plotting eigenvalues, US terminal breeds clustered separately in comparison with sheep from other locations of the world. Understanding the genetic relationships between terminal sire breeds in sheep will inform us about the potential applicability of markers derived in one breed to other breeds based on relatedness.

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Section: Discussionmentioning

confidence: 99%

Genetic structure and admixture in sheep from terminal breeds in the United States

Davenport

Hiemke²,

McKay

et al. 2020

Animal Genetics

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“…All sheep of sample sets 2 and 3 were phenotyped for horn status at an age at which horns are usually developed. In general, the names of the sheep breeds mentioned in this article refer to Mason and Porter [ 3 ], except for the breeds Travnička Pramenka [ 4 ], Alpines Steinschaf and Cameroon sheep (dad.fao.org).…”

Section: Methodsmentioning

confidence: 99%

“…Altamurana and Red Karaman, with females being strictly polled and males having or not having horns. Finally, there are some breeds in which the occurrence of horns varies both in males and females, for example in some strains of Steinschaf and of Pramenka such as Travnička Pramenka [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

The 1.78-kb insertion in the 3′-untranslated region of RXFP2 does not segregate with horn status in sheep breeds with variable horn status

Lühken

Krebs²,

Rothammer³

et al. 2016

Genet Sel Evol

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BackgroundThe mode of inheritance of horn status in sheep is far more complex than a superficial analysis might suggest. Observations, which were mostly based on crossbreeding experiments, indicated that the allele that results in horns is dominant in males and recessive in females, and some authors even speculated about the involvement of more than two alleles. However, all recent genome-wide association analyses point towards a very strong effect of a single autosomal locus on ovine chromosome 10, which was narrowed down to a putatively causal insertion polymorphism in the 3′-untranslated region of the relaxin/insulin-like family peptide receptor 2 gene (RXFP2). The main objective of this study was to test this insertion polymorphism as the causal mutation in diverse sheep breeds, including breeds with a variable and/or sex-dependent horn status.ResultsAfter re-sequencing a region of about 246 kb that covered the RFXP2 gene and its flanking regions for 24 sheep from six completely horned and six completely polled breeds, we identified the same insertion polymorphism that was previously published as segregating with horn status in these breeds. Multiplex PCR genotyping of 489 sheep from 34 breeds and some crosses between sheep breeds showed a nearly perfect segregation of the insertion polymorphism with horn status in sheep breeds of Central and Western European origin. In these breeds and their crossings, heterozygous males were horned and heterozygous females were polled. However, this segregation pattern was not, or at least not completely, reproducible in breeds with sex-dependent and/or variable horn status, especially in sheep that originated from even more southern European regions and from Africa. In such breeds, we observed almost all possible combinations of genotype, sex and horn status phenotype.ConclusionsThe 1.78-kb insertion polymorphism in the 3′-untranslated region of RXFP2 and SNPs in the 3′-UTR, exon 14 and intron 11 of this gene that we analyzed in this study cannot be considered as the only cause of polledness in sheep and are not useful as a universal marker to define the genetic horn status in sheep.Electronic supplementary materialThe online version of this article (doi:10.1186/s12711-016-0256-3) contains supplementary material, which is available to authorized users.

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“…Microsatellites have been widely used to analyze the genetic diversity and population structure of many traditional cattle breeds, including European unselected breeds (MEDUGORAC et al, 2009), Croatian (KEROS et al, 2013), Slovenian (SIMČIĆ et al, 2008, Spanish (MARTIN-BURRIEL et al, 2007), Indonesian (SUTARNO et al, 2015), Latin-American (DELGADO et al, 2011), Indian (DEPIKA and SALAR, 2014) and Zebu cattle (HUSSEIN et al, 2015;CHAUDHARI et al,2009). This approach has also been successfully used to analyze the genetic diversity in other traditional livestock species, such sheep (ĆURKOVIĆ et al, 2016), dogs (DIMITRIJEVIĆ et al, 2013), horses (ACHMANN et al, 2004). Some genetically characterization of Busha from Bosnia and Herzegovina have already reported (ROGIĆ et al, 2011b).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and structure of autochthonous cattle breeds from Bosnia and Herzegovina based on microsatellites

et al. 2019

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Genotype data from 21 microsatellites were used to evaluate genetic diversity and relationships of Gatacko cattle and Busha breeds. A total of 100 animals included in the study were divided into three groups: Gatacko cattle (GC), Busha from eastern Herzegovina (BEH) and Busha from western Herzegovina (BWH). Hardy-Weinberg equilibrium was observed for all loci in all groups, except for single locus (INRA35) in group of Busha from western Herzegovina. A total number of 166 alleles were detected in a total sample. The average number of alleles per locus was 7.9. The highest observed mean heterozygosity (0.688) was found in the Gatacko cattle and the lowest (0.634) in Busha from western Herzegovina. A pairwise population matrix of Fst values among analyzed groups showed significantly high genetic differentiation among all three groups: Gatacko cattle and Busha from western Herzegovina, Gatacko cattle and Busha from eastern Herzegovina and Busha from western and Busha from eastern Herzegovina. This report represents the first genetic characterization of Gatacko cattle and Busha cattle, traditional cattle breed from Bosnia and Herzegovina. The obtained results are valuable for the future development of conservation and management strategies for these cattle breeds.

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The genetic diversity and structure of 18 sheep breeds exposed to isolation and selection

Cited by 14 publications

References 18 publications

Genetic structure and admixture in sheep from terminal breeds in the United States

Genetic structure and admixture in sheep from terminal breeds in the United States

The 1.78-kb insertion in the 3′-untranslated region of RXFP2 does not segregate with horn status in sheep breeds with variable horn status

Genetic diversity and structure of autochthonous cattle breeds from Bosnia and Herzegovina based on microsatellites

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