The function of the nuclear matrix attachment region of silkworm rDNA as an autonomously replicating sequence in plasmid and chromosomal replication origin in yeast

Chen, Ying; Zhao, Ming; Li, Zai-Ping; He, Ming‐Liang

doi:10.1016/s0006-291x(02)02746-8

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…26,29 Alternatively, MARs may boost the function of transcriptional enhancers, 29,[33][34][35] and they may act as origins of replication when transcribed. [36][37][38][39][40] Finally, MARs may mediate changes in the structure of the chromatin to increase the accessibility of the promoter region to allow an increase of transcription, and to mediate sustained expression by counteracting DNA methylation and by supporting histone acetylation. 29,35,41,42 MAR elements have been previously shown to increase the stability and the expression of recombinant proteins from plasmids and viral vectors.…”

Section: Retroviral Vectors Represent An Important Tool In Human Genementioning

confidence: 99%

Use of human MAR elements to improve retroviral vector production

et al. 2010

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Section: Retroviral Vectors Represent An Important Tool In Human Genementioning

confidence: 99%

Use of human MAR elements to improve retroviral vector production

et al. 2010

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“…These "mini chromomeres" could be formed by smaller solenoid loops attached to matrix fibers. This is supported by investigations showing the length of a repetitive unit of 45S rDNA at an average of 8 kbp (Long and Dawid 1980) compared with 55 kbp for the estimated length of chromatin "loops" (Nelson et al 1986), as well as evidence that 45S rDNA genes contain matrix attachment regions in their nontranscribed spacer region (Dobbs et al 1994;Chen et al 2002). FluoroNanogold.…”

Section: Discussionmentioning

confidence: 74%

Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy

et al. 2005

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An atypical peg-like terminal constriction ("peg") on metaphase chromosomes of the plant genus Oziroë could be identified as a nucleolus organizing region (NOR) by detecting 45S rDNA with correlative light microscopy (LM) and scanning electron microscopy (SEM) in situ hybridization (ISH). Using high-resolution 3D analytical SEM, the architecture and DNA distribution of the peg-like NOR were characterized as typical for chromosomes, albeit with significantly smaller chromomeres. ISH procedure was improved for SEM concerning signal localization, labeling efficiency, and structural preservation, allowing 3D SEM analysis of the peg-like NOR structure and rDNA distribution for the first time. It could be shown that implementation of FluoroNanogold markers is an attractive tool that allows efficient immunodection in both LM and SEM. A model is proposed for the peg structure and its mode of condensation.

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“…Chromatin elements, like nuclear matrix attachment regions (MARs), have a crucial role in DNA replication. MARs identified in nontranscribed spacer (NTS) of silkworm Attacus ricini rDNA employ ARS activity in yeast, suggesting that the chromosome structure and the replication origin of rDNA have common features during evolution (Chen et al, 2002). The aquatic fungus B. emersonii is a organism that presents a special life cycle, genetic and biochemical characteristics similar to higher eukaryotic cells and therefore, it is suitable to be a study model to the origin of replication sites.…”

Section: Resultsmentioning

confidence: 99%

Isolamento e caracterização parcial de sequências homólogas a genes ribossomais (rDNA) em Blastocladiella emersonii

Gasques¹,

Fernandez

Corrêa³

2003

Acta Sci. Biol. Sci.

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ABSTRACT. The definition and the characterization of replication origins regions in higher eukaryotes are still controversial. The initiation of the replication is site-specific in some systems but seems to occur in large regions in others. Because of its in tandem organization, reducing the area to the restricted space that codifies an unit of transcription, rDNA regions are attractive models to study replication origins. In this work we isolated and started to characterize a clone that contains a ribosomal sequence from the aquatic fungus B. emersonii, Be97M20. Southern blots showed several sites for the restrition enzymes Eco RI, HindIII and SalI. A northern blot of total RNA, hybridized against a probe made from Be97M20, confirmed its homology with the ribosomal 18S gene. The detailed characterization, including complete restriction map, subcloning, sequence and analysis on bidimensional gels will provide important information about the structure and dynamics of this region.Key words: Blastocladiella emersonii, rDNA, ribosomal genes. RESUMO. Isolamento e caracterização parcial de sequências homólogas a genes ribossomais (rDNA) em Blastocladiella emersonii.A definição e a caracterização de regiões de origens de replicação nos eucariotos superiores são ainda controversas. A iniciação da replicação é sítio-específica em alguns sistemas e, em outros, parece estar contida em regiões extensas. Regiões rDNA são modelos atrativos para o estudo de origens de replicação pela sua organização in tandem, reduzindo a área de estudo para o espaço restrito que codifica uma unidade de transcrição. Neste trabalho nós isolamos e caracterizamos parcialmente um clone que contém uma sequência ribossomal do fungo aquático Blastocladiella emersonii, Be97M20. Southern blots mostraram diversos sítios para enzimas de restrição Eco RI, HindIII e SalI. Northern blot de RNA total hibridado contra uma sonda feita com Be97M20 confirmou a sua homologia com o gene ribossomal 18S. A caracterização detalhada, incluindo o mapeamento de restrição completo, subclonagem, sequenciamento e análise em géis bidimensionais proverão informações adicionais importantes sobre a estrutura e dinâmica desta região.

show abstract

The function of the nuclear matrix attachment region of silkworm rDNA as an autonomously replicating sequence in plasmid and chromosomal replication origin in yeast

Cited by 10 publications

References 32 publications

Use of human MAR elements to improve retroviral vector production

Use of human MAR elements to improve retroviral vector production

Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy

Isolamento e caracterização parcial de sequências homólogas a genes ribossomais (rDNA) em Blastocladiella emersonii

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