Use of human MAR elements to improve retroviral vector production

Identification of a potent MAR element from the mouse genome and assessment of its activity in stable and transient transfections

“…However, there have been a few reports of MAR increasing transgene expression within 48 h of transfection (Chancham et al, 2003;Kulkarni et al, 2004). We have also shown that the 1-68 MAR was able to improve retroviral vector titers in transient transfections in the human embryo HEK293 cell line (Buceta et al, 2011). Surprisingly, the S4 MAR rather had a minor negative impact on transient IgG expression, while it had no such effect when cloned in the pGEGFP vector (Fig.…”

Different matrix attachment regions flanking a transgene effectively enhance gene expression in stably transfected Chinese hamster ovary cells

“…The MARs of pCCF efficiently and stably increased the transgene expression level. Hence, the use of MAR elements opens new perspectives on the efficient generation of gene therapy vectors (Buceta et al, 2011). Other studies have illustrated that MARs may be used to mediate the sustained or higher expression levels of therapeutic genes, and/or to decrease the viral vector multiplicity of infection required to achieve consistent expression (Harraghy et al, 2008).…”

Characteristic element of matrix attachment region mediates vector attachment and enhances nerve growth factor expression in Chinese hamster ovary cells

“…There are two types of vectors: integrated and non-integrated vectors. Integrated vectors facilitate the insertion of mutations, and may be responsible for diseases arising as a result of the position of the inserted gene (Buceta et al, 2011;Ley et al, 2013). Ideal non-integrated vectors present no potential risk in gene therapy, are stable through mitosis, and express high levels of the transgene (Girod et al, 2007).…”