The Free Lipids of Brucella melitensis and Bordetella pertussis

Abstract

Free lipids were extracted from BrzLcella melitensis and Bordetella pertussis and their compositions compared with one another. The bacteria contained 1.60/, and 8.101, free lipids by dry weight of the respective species.The major component of the neutral lipids of Bruc. melitensis was coenzyme Qlo (0.6 pmollg bacterial dry weight). Smaller amounts of 1,2-and 1,3-diglycerides and of fatty acyl monoesters of ethanediol and of unknown or-glycols have been found among the neutral lipids of Bruc. melitensis. The major component of the neutral lipids of Bord. pertussis was a fraction of unesterified fatty acids. Moreover, coenzyme Qs (0.2 pmol/g bacterial dry weight) was identified in Bord. pertussis. Almost all saponifiable lipids of Bruc. melitensis contained considerable amounts of a GI,-cyclopropane acid (lactobacillic acid) whereas no cyclopropane acid was found among the unesterified fatty acids of Bord. pertussis.From the polar lipid fraction of Bruc. melitensis diphosphatidylglycerol (cardiolipin), phosphatidylglycerol, phosphatidylethanolamine (cephalin), phosphatidylcholin (lecithin) and phosphatidylserine were isolated ; the phosphatidylethanolamine fraction also contained phosphatidyl-N-methylethanolamine and phosphatidyl-N,N-Jimethylethanolamine. Phosphatidylcholine was the major component (37.6O/, of all phospholipids) in Bruc. melitensis. Determination of positional distribution of the component fatty acids revealed lactobacillic acid to be preferentially located in the number 2-positions of all phospholipids, while the I-positions were preferentially occupied by palmitic acid and in most phospholipids, also by hexadecenoic acid.From Bord. pertussis, diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylserine were isolated, but no phosphatidylcholine, no partially methylated phosphatidylethanolamine, and no phosphatidylinositol were found. Palmitic acid was located more frequently at the number 1-positions, hexadecenoic acid more frequently at the number .%positions of the phospholipids. Diphosphatidylglycerol and phosphatidylethanolamine probably contained minor amounts of a GI,-cyclopropane acid.An ornithine-containing lipid was isolated from Bruc. melitemis and Bord. pertussis. Besides ornithine these lipids contained ethanediol and fatty acids. A similar finding is recorded in Bruc. abortus. Ethanediol appeared to be ester linked to ornithine and to a fatty acid. Another fatty acid is probably linked to one of the amino groups of ornithine by an amide bond. I n Bruc. melitensis ond Bruc. abortus lactobacillic acid is predominantly ester linked, while palmitic and stearic acids are predominantly amide linked. There is no lactobacillic acid at all in the ornithine lipid of Bord. pertussis. No hydroxy fatty acid has been found in the ornithine lipids of any strain.From the lipid patterns of Bruc. melitensis, of Bruc. abortus studied previously, and of Bord. pertussis we conclude that it is probably not justified to include Brucellae and Bordetellae in the one natural family of Brucellacea...

Aurantimonas coralicida gen. nov., sp. nov., the causative agent of white plague type II on Caribbean scleractinian corals

“…The polar lipid compositions of members of the 'Rhizobiales' lineage are not entirely clear. All species investigated so far contain PE and PC in comparable amounts; DPG is also consistently present (Kaneshiro & Marr, 1962;Thiele et al, 1968;Bunn & Elkan, 1970;Thiele & Schwinn, 1973;Thompson et al, 1983;Kämpfer et al, 1999;Choma & Komaniecka, 2002). Either or both of PME and PDE may also be formed: this applies to Agrobacterium tumefaciens (Goldfine & Ellis, 1964), Rhizobium leguminosarum (Faizova et al, 1971), Brucella spp.…”

“…Either or both of PME and PDE may also be formed: this applies to Agrobacterium tumefaciens (Goldfine & Ellis, 1964), Rhizobium leguminosarum (Faizova et al, 1971), Brucella spp. (Thiele et al, 1968;Thiele & Schwinn, 1973;Kulikov & Dranovskaia, 1988), Sinorhizobium meliloti (Thompson Fig. 1.…”

Ornithine‐containing lipids in Thiobacillus A2 and Achromobacter sp.

“…The nitrogen content of thc ornithine lipid of Thiobucillus A2 was 3.96 % (the same value resulted from the molecular weight of the ornithine lipid which was calculated on the basis of the component fatty acids), the acyl ester content 1.34 pmoljmg (molar ratio nitrogeniacyl ester 2.1 :I). Provided the molecule is not [17]. The lysocompound was found to have a more polar character ( R , 0.30 on thin-layer chromatography with chloroform/methanol/water 95 :36 :6) than the original ornithine lipid ( R F 0.53).…”

Promotion of platelet aggregation by sera from brucellosis patients with antiphosphatidylcholine antibodies

“…The serum sample was absorbed twice with PC to reach the same level of inhibition as PAF (70%). Furthermore, the inhibition capacity of PAF was similar to that of the PYC group (PAF is an ether PYC) [7], suggesting that such activity could be attributed to its PYC group, as well as in PC (ether-PYC) [17]. Therefore, these results support an immunodominant role of PYC in the anti-PL and anti-PAF responses in brucellosis.…”

“…First, phospholipase A 2 associated with LDL or expressed by different cell types during in¯ammatory processes hydrolyses the fatty acid of the C 2 producing a smooth PC; secondly, when PC presents polyunsaturated fatty acids in the C 2 (this is the case with brucella PC) [17] it is oxidised, causing a short-chain PC. The molecules obtained during these processes (smooth PC and oxidised short chain PC) have similar structures to PAF.…”