The first outbreak of Taenia ovis infection in China

Shi, Wan-Gui; He, Wei; Guo, Xiaola; Liu, Quanyuan; Gao, Sheng-Zhi; Zhan, Fenghuang; Liu, Xu; Pan, Yuejun; Luo, Xuenong; Zheng, Yadong

doi:10.1016/j.parint.2016.06.005

Cited by 11 publications

(13 citation statements)

References 6 publications

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“…The Egyptian isolate of the current study is highly similar to other T. ovis isolates, as it shared 99% identity with the MT-CO1 gene of the T. ovis New Zealand isolate in GenBank AB731675.1 (NAKAO et al, 2013), of China in GenBank KU995334.1 (SHI et al, 2016) and other reference sequences (Figure 2), while…”

Section: Sequence Analysis and Phylogeny Of C Ovismentioning

confidence: 57%

“…However, the obtained prevalence is lower than those reported in sheep of Cairo, Egypt (5.7%) (ABEDL-MAOGOOD, 2005), in sheep of Western Australia (20.5%) (WHITE, 1976), in goats of eastern Ethiopia (22%) (SISSAY et al, 2008), in sheep of China (58.9%) (Shi et al, 2016), in sheep of Canada (48%) (DEWOLF et al, 2012) and in sheep of south-west England (7%) (EICHENBERGER et al, 2011).…”

Section: Discussionmentioning

confidence: 68%

“…Several earlier studies have been done concerning the molecular identification of T. ovis worldwide (DEWOLF et al, 2012;LAVIKAINEN et al, 2008LAVIKAINEN et al, , 2010SHI et al, 2016). Adding to this, the present study investigated the molecular identity of T. ovis in sheep of Upper Egypt and identified the possible origin of the parasite isolate.…”

Section: Discussionmentioning

confidence: 95%

“…The MT-CO1 gene was amplified by PCR using the following primer pairs: CO1-F 5′-ATGAATATTAAAACTTTATTAAGTTGGA-3′ and CO1-R 5′-TTAAACTAAAAAACCACGGGCA-3′, and this was performed with minor modifications according to methods previously described (Shi et al, 2016). PCR was done with the following conditions: 94 °C for 5 min, then followed by 30 cycles of 94 °C for 1 min, 50 °C for 35 s, 72 °C for 2 min and a final extension at 72 °C for 10 min.…”

Section: Pcr and Sequencing Of The Mitochondrial Mt-co1 Genementioning

confidence: 99%

“…The phylogram was constructed using the neighbor-joining method analysis of coding genes in the mitochondrial genome of T. ovis based on comparison and alignment with reference sequences of other species in Taeniidae (LAVIKAINEN et al, 2008;SHI et al, 2016) (Figure 2), and the tree was rooted with C. pisiformis (GU569096.1) using Kimura's two parameter model. The phylogram is drawn to scale, with branch lengths (next to the Molecular characterization of C. ovis from Egypt branches) in the same units as those of the evolutionary genetic distances (0.05%) (TAMURA et al, 2013).…”

Section: Assembling Of Nucleotide Sequences and Phylogenic Analysismentioning

confidence: 99%

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Molecular phylogenetic analysis of Cysticercus ovis from Egypt based on MT-CO1 gene sequences

Abdelaziz

Khalafalla

Hassan

et al. 2019

Rev. Bras. Parasitol. Vet.

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Cysticercus ovis or sheep measles is the larval stage of Taenia ovis, which is the intestinal tapeworm of dogs. It is found in the cardiac and skeletal muscles of sheep and can be the cause of partial or total condemnation of carcasses at abattoirs. The aim of the current work was to determine the prevalence of C. ovis among sheep in Upper Egypt and to present the molecular and phylogenetic analysis of this using the amplified Mitochondrial Cytochrome Oxidase subunit 1 (MT-CO1) gene. A total of 1885 sheep slaughtered at local abattoirs of 4 different governorates of Upper Egypt (Asuit, Sohag, Qena and Aswan) were carefully examined for C. ovis. The overall prevalence of infection was 2.02%. The highest rate of infection was observed in adult animals over 4 years of age (44.73%). There was no significant effect of animal sex on infection rates. The phylogenic analysis of C. ovis Egyptian isolates showed very close similarity to the New Zealand isolate (AB731675). This is the first report showing the genetic analysis of C. ovis in Egypt, which provides a very powerful tool for taxonomy and definitive diagnosis of C. ovis, which could be helpful for preventive and control programs.

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Section: Sequence Analysis and Phylogeny Of C Ovismentioning

confidence: 57%