The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula

Abstract

We report the first gene-based linkage map of Lupinus angustifolius (narrow-leafed lupin) and its comparison to the partially sequenced genome of Medicago truncatula. The map comprises 382 loci in 20 major linkage groups, two triplets, three pairs and 11 unlinked loci and is 1,846 cM in length. The map was generated from the segregation of 163 RFLP markers, 135 gene-based PCR markers, 75 AFLP and 4 AFLP-derived SCAR markers in a mapping population of 93 recombinant inbred lines, derived from a cross between domesticated and wild-type parents. This enabled the mapping of five major genes controlling key domestication traits in L. angustifolius. Using marker sequence data, the L. angustifolius genetic map was compared to the partially completed M. truncatula genome sequence. We found evidence of conserved synteny in some regions of the genome despite the wide evolutionary distance between these legume species. We also found new evidence of widespread duplication within the L. angustifolius genome.

Prins and C-PRINS: Promising tools for the physical mapping of the lupin genome

“…All these cytogenetic chromosome markers together will enable us to construct the karyotype of L. angustifolius. PRINS and C-PRINS will also be useful for the localisation of marker sequences from the genetic maps of the narrow-leafed lupin [22,23] on its chromosomes. That will allow the verification of linkage groups and the integration of the physical and genetic maps of L. angustifolius.…”

Development of two sequence-specific PCR markers linked to the le gene that reduces pod shattering in narrow-leafed Lupin (Lupinus angustifolius L.)

“…Part of the resulting RIL population has previously been used to produce linkage maps for L. angustifolius Nelson et al, 2006).…”

“…Recently, two molecular genetic maps have been produced in L. angustifolius on which le has been mapped Nelson et al, 2006). No marker was located within 5 centiMorgans (cM) of the le gene on the map of Nelson et al (2006).…”

Pod Shattering: A Homologous Series of Variation Underlying Domestication and an Avenue for Crop Improvement

“…Genetic analysis of pod shattering has been carried out in various legumes including soybean [48,[93][94][95][96][97][98][99][100][101], common bean [13,44], pea [18,50,102], cowpea [49,[103][104][105][106], lentil [16,46,107], narrow-leaf lupin [47,108], azuki bean [45,109], and common vetch [43,110]. In most of the legumes that have been studied so far, pod shattering is found to be a dominant trait controlled by one or two genes or QTLs (Table 1).…”

“…Similar to common vetch, a single gene controls the shattering process in azuki bean, Vigna angularis [45,109]. In narrow-leafed lupin (Lupinus angustifolius), two genes are involved in controlling the recessive non- shattering trait [108]. One of the two genes, lentus (le) affects the pod endocarp orientation, reducing the mechanical pressure required for pod shattering, while the second gene tardus (ta) causes the dorsal and ventral pod seams to be fused, inhibiting the separation of the two halves [47].…”