“…Fluorescence in situ hybridization (FISH) has been conventionally used to integrate genetic and chromosomal maps in several plant species (Jiang et al, 1995;Dong et al, 2000;Sadder et al, 2000;Cheng et al, 2001a,b;Kulikova et al, 2001;Howell et al, 2002;Islam-Faridi et al, 2002;Kim et al, 2002;Pedrosa et al, 2002;Koumbaris and Bass, 2003;Zhang et al, 2005;Wang et al, 2008). Because of the combination of accuracy and sensitivity of polymerase chain reaction (PCR) with FISH, primed in situ labeling (PRINS) has been found to be a more powerful tool for localizing DNA sequences, single-copy genes (Macas et al, 2000;Kubaláková et al, 2001;Tatum and Rayburn, 2006;Kaczmarek et al, 2007;Gao et al, 2011), and restriction fragment length polymorphism loci (Zhu et al, 1995;Shi et al, 1996) in some plants (Birchler and Danilova, 2012).…”