The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)

Meng, Elaine C.; Tang, Baozhen; Sánchez‐García, Francisco Javier; Qiao, Ting; Fu, Lang; Wang, Yu; Wu, Jianglin; Chen, Zhiming

doi:10.3390/v11030257

Cited by 4 publications

(3 citation statements)

References 50 publications

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“…AcNSRV-1 and AcNSRV-2 belong to family Lispiviridae of the order Mononegavirales, whereas LdNSRV-1 belongs to a big family Rhabdoviridae. Sixteen viral genome sequences which belong to family Lispiviridae (3, found in hymenopteran orussidae, cynipidae, or aphelinidae insects, respectively) and Rhabdoviridae (13, found in hymenopteran eulophidae, sphecidae, chrysididae, pompilidae, sapygidae, or mymaridae insects, respectively) have been identified in the hymenopteran insects (32)(33)(34). The impact of these viruses on the host wasps has rarely been determined.…”

Section: Discussionmentioning

confidence: 99%

Diverse RNA Viruses Discovered in Three Parasitoid Wasps of the Rice Weevil Sitophilus oryzae

Wang

Yuan

Xiao

et al. 2021

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In this study, many virus-like fragments were obtained from transcriptomes of three wasp species, including Anisopteromalus calandrae (8), Lariophagus distinguendus (3), and Theocolax elegans (18), which can parasitize and control rice weevil Sitophilus oryzae, a serious insect pest of farm-stored grains. By further bioinformatic analysis and sequencing, we identified six novel RNA viruses with complete genomes and named them WWPSRV-1, WWPSRV-2, AcPSRV-1, AcNSRV-1, AcNSRV-2, and LdNSRV-1. PCR-based detection revealed that WWPSRV-1 and WWPSRV-2 had the possibility of interspecies virus transmission, especially WWPSRV-2, which was also present in the rice weevil adults. Phylogenetically, three out of these six viruses appeared to be members of order Picornavirales: WWPSRV-1 belonged to unassigned virus families of this order, whereas WWPSRV-2 and AcPSRV-1 belonged to families Iflaviridae and Dicistroviridae, respectively. The conserved picornavirus-typical domains helicase, protease, and RNA-dependent RNA polymerase could be found in the nonstructural protein encoded by the three viruses, whose genomes consisted of the different numbers of open reading frames (ORFs). The other three RNA viruses could be classified to order Mononegavirales: AcNSRV-1 and AcNSRV-2 belonged to family Lispiviridae, whereas LdNSRV-1 belonged to a big family Rhabdoviridae. The genomes of the three viruses contained at least five ORFs, encoding deduced proteins in the following order: 3′-N-P-M-G-L-5′. All the ORFs were separated by conserved intergenic sequences which likely regulated the transcription termination and initiation. Our findings enhance the understanding of RNA viruses in weevil wasps and set the foundation for the future study of the association among weevils, weevil wasps, and RNA viruses. IMPORTANCE The enormous diversity of RNA viruses in insects is continuously validated. Parasitoid wasps, as biocontrol insects which are widely used against insect pests in agroecosystems, may also carry many “good” RNA viruses. Some RNA viruses in parasitoid wasps have been reported to affect the host wasps or the wasps’ host. Here, six novel RNA viruses with complete genomes were identified in three parasitoid wasps of the rice weevil. One of these viruses was also detected in the rice weevil adults. Phylogenetically, WWPSRV-1 was the first unambiguous detection of Nora-like virus in insect parasitoids. WWPSRV-2 and AcPSRV-1 belong to families Iflaviridae and Dicistroviridae, some viruses of which can result in lethal infections in silkworms and honeybees. The other three RNA viruses belong to order Mononegavirales, which comprises many well-known insect-associated viruses.

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Section: Discussionmentioning

confidence: 99%

Diverse RNA Viruses Discovered in Three Parasitoid Wasps of the Rice Weevil Sitophilus oryzae

Wang

Yuan

Xiao

et al. 2021

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“…In a comprehensive study, 68 novel and nine previously reported viral sequences were characterized in the transcriptomes of V. vulgaris colonies from both their native range (Belgium) and an invasive range (New Zealand) [34] . RNA viruses have also been identified in parasitic wasps, which are natural enemies of insect pests [33] , [37] , [38] , [39] . Transcriptomic analysis of three wasp species, Anisopteromalus calandrae , Lariophagus distinguendus , and Theocolax elegans , known for parasitizing and controlling the rice weevil Sitophilus oryzae , a major insect pest of stored grains, led to the discovery of six novel RNA viruses [33] .…”

Section: Rna Viruses In Agricultural Insects Of Different Ordersmentioning

confidence: 99%

Diversity of RNA viruses in agricultural insects

Qi,

Ye,

Zhang

et al. 2023

Computational and Structural Biotechnology Journal

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“…Several attempts were made by different studies to control this beetle using non-chemical methods [ 17 , 18 , 19 ]. One of these was the use of Tetrastichus brontispae Ferrière (Hymenoptera: Eulophidae) [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ] entomopathogenic nematode Steinernema carpocapsae [ 17 , 18 , 19 ] and Metarrhizium anisopliae [ 29 , 30 , 31 , 32 ]. More eco-friendly and cost-effective methods of control of O. nipae are needed.…”

Section: Introductionmentioning

confidence: 99%

The Entomopathogenic Nematodes H. bacteriophora and S. carpocapsae Inhibit the Activation of proPO System of the Nipa Palm Hispid Octodonta nipae (Coleoptera: Chrysomelidae)

Sanda

Hou

2022

Life

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Entomopathogenic nematodes are biocontrol agents of invasive insect pests in soil and cryptic habitats. Nipa palm hispid, Octodonta nipae, is a pest of palm trees in Sothern China. To address its increasing damage, environmentally friendly control methods are required. This study aimed to test efficacy of Heterorhabditis bacteriophora and Steinernema carpocapsae on O. nipae and investigated the influence of secondary metabolites, nematodes, and their isolated cuticles on the activation of O. nipae’s prophenoloxidase system using qPCR analysis. Our data revealed that O. nipae were less susceptible to H. bacteriophora than S. carpocapsae and penetrations of infective juveniles were higher with S. carpocapsae treatment than H. bacteriophora. Moreover, expression levels of the serine protease P56, prophenoloxidase activation factor 1, PPO and serine protease inhibitor 28 upon S. carpocapsae and H. bacteriophora infections were generally downregulated at all times. However, upon heating, the cuticles lost their inhibitory effects and resulted in upregulation of the PPO gene. Similarly, the addition of arachidonic acid reversed the process and resulted in the upregulation of the PPO gene compared to the control. Further work is needed to identify toxic substances secreted by these EPNs to evade O. nipae’s immune system.

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The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)

Cited by 4 publications

References 50 publications

Diverse RNA Viruses Discovered in Three Parasitoid Wasps of the Rice Weevil Sitophilus oryzae

Diverse RNA Viruses Discovered in Three Parasitoid Wasps of the Rice Weevil Sitophilus oryzae

Diversity of RNA viruses in agricultural insects

The Entomopathogenic Nematodes H. bacteriophora and S. carpocapsae Inhibit the Activation of proPO System of the Nipa Palm Hispid Octodonta nipae (Coleoptera: Chrysomelidae)

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