Diversity of RNA viruses in agricultural insects

Qi, Yu-Hua; Ye, Zhuang-Xin; Zhang, Chuan-Xi; Chen, Jian-Ping; Li, Jun-Min

doi:10.1016/j.csbj.2023.08.036

Cited by 3 publications

(1 citation statement)

References 154 publications

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“…Besides causing direct sucking damage, BPH also acts as a vector for two important rice viruses, rice ragged stunt virus (RRSV) and rice grassy stunt virus (RGSV), leading to substantial reductions in crop yield [2,3]. Apart from plant viruses transmitted by BPH, several BPH-speci c viruses have been identi ed and belong to the different virus families, such as I aviridae (Nilaparvata lugens honeydew virus, NLHV), Dicistroviridae (Himetobi P virus, and Nilaparvata lugens C virus), and Reoviridae (Nilaparvata lugens reovirus) [4][5][6][7].…”

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confidence: 99%

Complete sequence and genetic characterization of a novel segmented RNA virus infecting Nilaparvata lugens

Ning,

Ye,

et al. 2024

Preprint

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The brown planthopper (BPH), Nilaparvata lugens, is a significant agricultural pest capable of long-distance migration and transmitting severe rice viruses. This study identified a novel segmented RNA virus in BPH, displaying a close homology to members of a recently discovered virus lineage known as "Quenyaviruses" within the Orthornavia virus kingdom. This newly identified virus was named "Nilaparvata lugens Quenyavirus" (NLQV). NLQV consists of five positive-sense, single-stranded RNAs (+ ssRNAs), with each segment encoding a single open reading frame (ORF). The genomic characteristics and phylogenetic analysis support the classification of NLQV as a novel member of the Quenyaviruses. Notably, all NLRV segments share the 5'-terminal sequence AUCUG. The characteristic vsiRNA profile of NLQV suggests that the antiviral RNAi pathway of host BPH was activated in response to virus infection. Remarkably, these findings represent the first documented report of Quenyaviruses in planthoppers, contributing to our understanding of Quenyaviruses and expanding our knowledge of insect-specific viruses in planthoppers.

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Section: Full Textmentioning

confidence: 99%

Complete sequence and genetic characterization of a novel segmented RNA virus infecting Nilaparvata lugens

Ning,

Ye,

et al. 2024

Preprint

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Identification and Characterization of Three Novel Solemo-like Viruses in the White-Backed Planthopper, Sogatella furcifera

Yuan,

Ye,

Chen

et al. 2024

Insects

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Agricultural insects play a crucial role in transmitting plant viruses and host a considerable number of insect-specific viruses (ISVs). Among these insects, the white-backed planthoppers (WBPH; Sogatella furcifera, Hemiptera: Delphacidae) are noteworthy rice pests and are responsible for disseminating the southern rice black-streaked dwarf virus (SRBSDV), a significant rice virus. In this study, we analyzed WBPH transcriptome data from public sources and identified three novel viruses. These newly discovered viruses belong to the plant-associated viral family Solemoviridae and were tentatively named Sogatella furcifera solemo-like virus 1-3 (SFSolV1-3). Among them, SFSolV1 exhibited a prevalent existence in different laboratory populations, and its complete genome sequence was obtained using rapid amplification of cDNA ends (RACE) approaches. To investigate the antiviral RNA interference (RNAi) response in WBPH, we conducted an analysis of virus-derived small interfering RNAs (vsiRNAs). The vsiRNAs of SFSolV1 and -2 exhibited typical patterns associated with the host’s siRNA-mediated antiviral immunity, with a preference for 21- and 22-nt vsiRNAs derived equally from both the sense and antisense genomic strands. Furthermore, we examined SFSolV1 infection and distribution in WBPH, revealing a significantly higher viral load of SFSolV1 in nymphs’ hemolymph compared to other tissues. Additionally, in adult insects, SFSolV1 exhibited higher abundance in male adults than in female adults.

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Bactericera cockerelli Picorna-like Virus and Three New Viruses Found Circulating in Populations of Potato/Tomato Psyllids (Bactericera cockerelli)

Dahan,

Orellana,

Wald

et al. 2024

Viruses

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An investigation of viruses circulating in populations of field and laboratory potato/tomato psyllids (Bactericera cockerelli) was conducted using high-throughput sequencing (HTS) technology and conventional RT-PCR. Three new viruses were discovered: one from the family Tymoviridae and two from the family Solemoviridae. A tymo-like virus sequence represented a nearly complete 6843 nt genome of a virus named Bactericera cockerelli tymo-like virus (BcTLV) that spanned five open reading frames (ORFs) which encoded RNA-dependent RNA polymerase (RdRP), helicase, protease, methyltransferase, and a capsid protein. Phylogenetic analyses placed the RdRP of BcTLV inside a divergent lineage of the viruses from the family Tymoviridae found in insect and plant hosts in a sister clade to the genera Tymovirus, Marafivirus, and Maculavirus. Four solemo-like virus sequences were identified in the HTS outputs, representing two new viruses. One virus found only in field-collected psyllids and named Bactericera cockerelli solemo-like virus 1 (BcSLV-1) had a 5479 nt genome which spanned four ORFs encoding protease and RdRP. Three solemo-like sequences displayed 87.4–99.7% nucleotide sequence identity among themselves, representing variants or strains of the same virus named Bactericera cockerelli solemo-like virus 2 (BcSLV-2). The genome of BcSLV-2 spanned only two ORFs that encoded a protease and an RdRP. Phylogenetic analysis placed the RdRPs of BcSLV-1 and BcSLV-2 in two separate lineages as sister clades to viruses from the genus Sobemovirus found in plant hosts. All three new psyllid viruses were found circulating in psyllids collected from potato fields in southern Idaho along with a previously identified Bactericera cockerelli picorna-like virus. Any possible role of the three viruses in controlling populations of the field psyllids remains to be elucidated.

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Diversity of RNA viruses in agricultural insects

Cited by 3 publications

References 154 publications

Complete sequence and genetic characterization of a novel segmented RNA virus infecting Nilaparvata lugens

Complete sequence and genetic characterization of a novel segmented RNA virus infecting Nilaparvata lugens

Identification and Characterization of Three Novel Solemo-like Viruses in the White-Backed Planthopper, Sogatella furcifera

Bactericera cockerelli Picorna-like Virus and Three New Viruses Found Circulating in Populations of Potato/Tomato Psyllids (Bactericera cockerelli)

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