No abstract

Nigeria is the largest producer of tomato in Africa while the Northern regions are the main producers of tomatoes in the country. Tomato (Lycopersicon esculentum Mill.) is widely cultivated and consumed in every part of Nigeria. It is an important commercial crop and good source of vitamins, minerals, essential amino acids, sugar and dietary fibers. The study aimed to report the outbreak of Tomato leafminer (Tuta absoluta) in tomato growing regions of Nigeria with a view to bring out its management strategies in Nigeria. The study was conducted in the major tomato

Bacterial symbionts of insects affect a wide array of host traits including fitness and immunity. Octodonta nipae (Maulik), commonly known as hispid leaf beetle is a destructive palm pest around the world. Understanding the dynamics of microbiota is essential to unravel the complex interplay between O. nipae and its bacterial symbionts. In this study, bacterial 16S rRNA V3-V4 region was targeted to decipher the diversity and dynamics of bacterial symbionts across different life stages [eggs, larvae, pupae, and adult (male and female)] and reproductive organs (ovaries and testis) of O. nipae. Clustering analysis at ≥97% similarity threshold produced 3,959 operational taxonomic units (OTUs) that belonged to nine different phyla. Proteobacteria, Actinobacteria, and Firmicutes represented the bulk of taxa that underwent notable changes during metamorphosis. Enterobacteriaceae and Dermabacteraceae were the most abundant families in immature stages (eggs, larvae, and pupae), while Anaplasmataceae family was dominated in adults (male and female) and reproductive organs (ovaries and testis). The genus Serratia and Lactococcus were most abundant in eggs, whereas Pantoea and Brachybacterium represented the bulk of larvae and pupae microbiota. Interestingly the genus Wolbachia found positive to all tested samples and was recorded extremely high (>64%) in the adults and reproductive organs. The bacteria varied across the developmental stages and responsible for various metabolic activities. Selection choice exerted by the insect host as a result of its age or developmental stage could be the main reason to ascertain the shift in the bacteria populations. Maternally inherited Wolbachia was found to be an obligate endosymbiont infecting all tested life stages, body parts, and tissues. These outcomes foster our understanding of the intricate associations between bacteria and O. nipae and will incorporate in devising novel pest control strategies against this palm pest.

The humoral immune responses of the nipa palm hispid beetle Octodonta nipae involves the inducible expression of the genes coding for antimicrobial peptides (AMPs) which are mediated by immune deficiency signaling pathways. In insects, the nuclear factor-κB (NF−κB) transcription factor, Relish, has been shown to regulate AMP gene expressions upon microbial infections. Here, we dissect the expression patterns of some AMPs in O. nipae during infections by entomopathogenic nematodes (EPNs) and their symbionts, before and after Relish knock down. Our results indicate that, prior to gene silencing, the AMPs attacin C1, attacin C2, and defensin 2B were especially expressed to great extents in the insects challenged with the nematodes Steinernema carpocapsae and Heterorhabditis bacteriophora as well as with their respective symbionts Xenorhabdus nematophila and Photorhabdus luminescens. The study also established the partial sequence of OnRelish/NF-κB p110 subunit in O. nipae, with an open reading frame coding for a protein with 102 amino acid residues. A typical Death domain-containing protein was detected (as seen in Drosophila) at the C-terminus of the protein. Phylogenetic analysis revealed that in O. nipae, Relish is clustered with registered Relish/NF-κB p110 proteins from other species of insect especially Leptinotarsa decemlineata from the same order Coleoptera. Injection of OnRelish dsRNA remarkably brought down the expression of OnRelish and also reduced the magnitude of transcription of attacin C1 and defensin 2B upon S. carpocapsae and H. bacteriophora and their symbionts infections. Altogether, our data unveil the expression pattern of OnRelish as well as that of some AMP genes it influences during immune responses of O. nipae against EPNs and their symbionts.