The features of morphological changes in the urinary bladder under combined effect of heavy metal salts

RomaniukAnatolii,; SikoraVladyslav,; LyndinMykola,; SmiyanovVladyslav,; SikoraVolodymyr,; Lyndina, Yuliia Mykolaivna; Piddubnyi, Artem; GyryavenkoNataliya,; KorobchanskaAnna,

doi:10.1556/1646.9.2017.09

Cited by 15 publications

(34 citation statements)

References 14 publications

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“…In our previous histological and immunohistochemical researches of UB, we demonstrated that HM impact is the cause of significant morphological transformations in all structural components of the organ with the specific features of each layer. Furthemore, we have shown that the sequences of intensity and depth of pathological lesions were dependent on the duration of the experiment 18 . Nevertheless, more recent research has shown that the that long-term of HMS exposure leads to increases of HM accumulation level in the UB tissue which influence significantly on the morphometric parameters of organ wall thickness (this data has not been published yet).A lot of simulated studies demonstrated that the organ easily exposed to development of inflammatory diseases of various genesis that accompanied by urodynamic disorders 15,17 .…”

Section: Discussionmentioning

confidence: 85%

“…Interestingly, that UB can be attributed to a few organs that fall under the indirect hematogenous and direct contact impact of HM ions that enter the body in the urine and blood 11,19 . Due to this, the metal-microelements can cause various variation of morphological changes in the UB during subacute and chronic periods of intoxication 18,20 . In our previous histological and immunohistochemical researches of UB, we demonstrated that HM impact is the cause of significant morphological transformations in all structural components of the organ with the specific features of each layer.…”

Section: Discussionmentioning

confidence: 99%

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Effect of heavy metals on the readaptive processes in the urinary bladder

Романюк¹,

Lуndіna²,

Lуndіn³

et al. 2018

Bangladesh J Med Sci

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Introduction: Heavy metals (HM) are dangerous elements due to their toxicity and prevalence play an important role in the environmental pollution and hazards to health risk. Impact of HM at high concentrations may lead to the failures of urinary bladder (UB) structure and functions. The aim of our study was to determine the readaptive changes in the UB of the rats and effectiveness of vitamin E using after the long-term intake of the HMS mixtures. Materials and Methods: We used mature male laboratory rats which were divided into three series with special experimental conditions after the influence of HM. The detailed analysis of the UB state was performed by using morphometric, spectrophotometric, histological and electron microscopy methods. Results: We had determined that the long-term intake of HM caused the significant increase of their accumulation in the tissue and morphometric changes of UB. Obtained results after the cancelation of the HM effect demonstrate smooth morphological restructuring of the UB wall with the reduction of difference of accumulation and morphometric indicators with control. The adjustment of vitamin E during there adaptation period showed better result recovery and optimization of all parameters, but they haven’t completely returned to normal state. Conclusions: The influence of HMS mixture led to significant negative effect on the UB at all stages of research. However, under the conditions of readaptation the tendency towards a certain stabilization of all indicators was observed, but they didn’t completely pass to the end of the study. The vitamin E using has caused the acceleration and improved the development of readaptive change after HM exposure. Bangladesh Journal of Medical Science Vol.18(1) 2019 p.100-106

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Effect of heavy metals on the readaptive processes in the urinary bladder

Романюк¹,

Lуndіna²,

Lуndіn³

et al. 2018

Bangladesh J Med Sci

Self Cite

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“…Different degrees of severity of inflammation are detected in the biopsy specimens of the bladder; however, cellular mechanisms of inflammation in IC/BPS and the pro-cesses leading to tissue damage and fibrosis are not yet clear (6,7). Among many suggested causes, mast cell activation is noteworthy (8).…”

Section: Introductionmentioning

confidence: 99%

Infiltration of Lymphocytes and Mast Cells to Bladder Tissues in Experimental Models of Interstitial Cystitis/Bladder Pain Syndrome

оглы

2020

Nephro-Urol Mon

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Background: A different degree of severity of inflammation was detected in the bladder biopsy specimens; however, the cellular mechanisms of inflammation in interstitial cystitis/bladder pain syndrome (IC/BPS) and the processes leading to tissue damage and fibrosis are not yet completely clear. Objectives: The aim of this study was to determine the leukocyte and mast cell infiltration to the bladder tissue in various experimental models of IC/BPS. Methods: Various IC/BPS models have been generated using white New Zealand rabbits. Animals were divided into 4 groups depending on the type of the model: (1) The introduction of 0.5% HCl into the bladder; (2) The introduction of urine taken from the bladder into the wall of the bladder; (3) The introduction of 0.9% NaCl; (4) Intact animals. The number of cells in the tissues of the bladder was evaluated in points and histological analysis of the tissues of the bladder wall was performed. The analysis was carried out after 14 days of modeling. Results: Mast cells were determined only in groups 1 and 2 (P < 0.001). In the 2nd group, developing necrosis and mixed inflammatory infiltration, giant, necrotic, multinucleated histiocytes were noted. In group 3, tissue edema and single scattered lymphocytes were noted. Conclusions: The association of high inflammatory cell infiltration in the chemical version of the IC/BPS model and mast cell infiltration with impaired bladder integrity has been established.

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“…However, the nature of the disease and the symptoms may vary, depending on the provocative factors: factors of the modern lifestyle (smoking, alcohol, chronic stress), the influence of the pollutants (anthropogenic pollution), the presence of the pathogenic microorganisms (Escherichia coli, Clostridium botulinum, etc.) and pathological processes in the UB wall (innervation disorders, cystitis and cancer) [3,6,7,10,11].…”

Section: Introductionmentioning

confidence: 99%

Variability of T-Lymphocytes and Macrofags Composition in the Urinary Bladder Under the Influence of Heavy Metals Salts, Correction of Their Effect With Vitamin E and in the Readaptation Period

Sikora

2018

Rep. of Morph.

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In modern urology, it is important to study and understand the features of the development of various pathologies in the organs of the urinary system since the disturbances of the urodynamics is a widespread problem all over the world. Thus, the environment pollution caused by heavy metal salts has unpredictable consequences for the functioning of the urinary bladder. That is why the object of this study is to determine the features of the expression and the quantitative composition of T-cells and macrophages in the UB wall under the intake of the HMS and correction of their effect with vitamin E, as well as during the readaptation period. According to the experimental scenario, the experiment model was implemented by using male rats that were divided into six groups. The heavy metal salts mixture consisted of the most common potentially dangerous metals-microelements (zinc, cooper, iron, manganese, lead and chrome). Vitamin E was chosen as a corrector. To determine the quantitative composition of T-cells and macrophages the immunohistochemical identification by CD3 and CD68 antibodies was used. The main results are presented as M±SD, with the assessment of the differences and dependencies between the groups by using nonparametric Mann-Whitney U test and Spearman’s Rank Correlation Coefficient. We found that the number of CD3+ lymphocytes and CD68+ macrophages differed between the groups and depended on the experimental conditions. Thus, the results of our study demonstrate that the number of T-cells and macrophages in the bladder increases under the influence of heavy metals salts. The simultaneous intake of the pollutants and vitamin E is accompanied by the decrease of expression intensity of the corresponding cells. When the intake of the heavy metals is canceled, the qualitative composition of the identified CD3 and CD68 cells gradually comes to the norm, but the speed of its reduction differs depending on the group of the readaptation. Discovered that the decrease of T-cells and macrophages in the groups with the use of vitamin E was more approached to the control. Based on the results of the immunohistochemical study, a strong correlation between an increased number of the expressed T-cells and macrophages was revealed in the urinary bladder during the whole period of the study that demonstrates the dependence of their activity.

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The features of morphological changes in the urinary bladder under combined effect of heavy metal salts

Cited by 15 publications

References 14 publications

Effect of heavy metals on the readaptive processes in the urinary bladder

Effect of heavy metals on the readaptive processes in the urinary bladder

Infiltration of Lymphocytes and Mast Cells to Bladder Tissues in Experimental Models of Interstitial Cystitis/Bladder Pain Syndrome

Variability of T-Lymphocytes and Macrofags Composition in the Urinary Bladder Under the Influence of Heavy Metals Salts, Correction of Their Effect With Vitamin E and in the Readaptation Period

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