Relevance. Interstitial cystitis/painful bladder syndrome (IC/PBS) is a debilitating condition of pain and discomfort in the bladder. Due to the limited number of published studies in this area, there is a need for further research. Objective: to evaluate the level of nerve growth factor and its relationship with various types of leukocytes and mast cells of the bladder tissue in animals with experimental models of interstitial cystitis/painful bladder syndrome. Material and methods. IC/PBS modeling was performed on 38 female rabbits. The IC/PBS modeling was created by introducing HCl into the bladder (group 1), urine into the wall of the bladder (group 2), and physiological saline (group 3). Nerve growth factor (NGF) was determined by the ELISA method, and white blood cells along with mast cells in tissues were determined by histological processing. Results. A statistically significant high level of NGF in blood and urine was observed on the 1st day after IC/PBS initiation in groups 13 and in group 2 respectively. Two weeks after the initiation of IC/PBS in animals of group 1 a decrease in the level of FRN in the blood by 29.3% and its increase in urine by 14.3% was observed; in group 2 an increase by 65.5% (p 0.01) in blood and by 52.7% (p 0.01) in urine was observed and in group 3 a decrease by 30.8% (p 0.05) in blood and by 30.5% (p 0.05) in urine was observed. The greatest number of leukocytes was determined in the tissue of the bladder in animals of the 1st group. Mast cell infiltration was observed in groups 1 and 2. Correlation between the NGF in blood and urine and between NGF and the number of leukocytes and mast cells was revealed. Findings. In IC/PBS, the level of NGF in the blood and urine is increased. Indicators of nerve growth factor in blood and urine are correlated with multidirectional connections. High mast cell infiltration occurs when damage to the integrity of the bladder by urinary toxicity. Nerve growth factor correlates with leukocyte and mast cell infiltration in the bladder tissue. Conclusion. During IC/PBS, the level of NGF in the blood and urine is increased. Levels of nerve growth factor in blood and urine are correlated with multidirectional connections. High mast cell infiltration occurs as a response to the damage of the bladder wall integrity caused by urinary toxicity. The level of nerve growth factor correlates with the bladder tissue intensity of infiltration with leukocytes and mast cells.
Background: A different degree of severity of inflammation was detected in the bladder biopsy specimens; however, the cellular mechanisms of inflammation in interstitial cystitis/bladder pain syndrome (IC/BPS) and the processes leading to tissue damage and fibrosis are not yet completely clear. Objectives: The aim of this study was to determine the leukocyte and mast cell infiltration to the bladder tissue in various experimental models of IC/BPS. Methods: Various IC/BPS models have been generated using white New Zealand rabbits. Animals were divided into 4 groups depending on the type of the model: (1) The introduction of 0.5% HCl into the bladder; (2) The introduction of urine taken from the bladder into the wall of the bladder; (3) The introduction of 0.9% NaCl; (4) Intact animals. The number of cells in the tissues of the bladder was evaluated in points and histological analysis of the tissues of the bladder wall was performed. The analysis was carried out after 14 days of modeling. Results: Mast cells were determined only in groups 1 and 2 (P < 0.001). In the 2nd group, developing necrosis and mixed inflammatory infiltration, giant, necrotic, multinucleated histiocytes were noted. In group 3, tissue edema and single scattered lymphocytes were noted. Conclusions: The association of high inflammatory cell infiltration in the chemical version of the IC/BPS model and mast cell infiltration with impaired bladder integrity has been established.
Интерстициальный цистит, или болевой синдром мочевого пузыря, -хроническое воспалительное заболева-ние мочевого пузыря с неизвестной этиологией. Оно ухудшает качество жизни, вызывает депрессию, стресс, нарушение сексуальных функций. Несмотря на многочисленные исследования, этиология интерстициально-го цистита до сих пор неясна, и болезнь рассматривают как синдром раздражённого мочевого пузыря с муль-тифакториальным генезом. Согласно Национальному анкетированию по вопросам здоровья и питания США,
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