The Feasibility of Using Blood Bank-Stored (4°C) Cord Blood, Unmatched for HLA for Marrow Transplantation

Abstract

The main purpose for storing large numbers of umbilical cord blood (CB) Previous results 4,5 have indicated that CB stored for 5 to 21 days under blood bank conditions at 4°C will rescue mice from the effects of lethal irradiation by acting as a temporary bridge and by stimulating endogenous hematopoiesis leading to self-reconstitution. 6 Furthermore, in methylcellulose cultures, blast colonies were generated from 21-day-old stored CB, and replating these colonies produced secondary colonies 62.5% of the time. 4If CB can be stored in existing blood bank facilities at 4°C and does not require a close HLA match for successful transplantation, it would transform what is now a valuable biologic commodity with limited availability into one that is widely available to many more patients, children and adults, at a much more affordable cost. Moreover, for developing countries, which currently do not have the necessary financial resources to establish frozen CB banks or have cultural barriers, this would be a major step forward in providing a source of hematopoietic cells for transplantation.The studies described herein were undertaken to further evaluate the effects of storage of human umbilical CB

Effect of IGF-1 and Uncultured Autologous Bone-Marrow-Derived Mononuclear Cells on Repair of Osteochondral Defect in Rabbits

“…MSCs have both osteogenic and chondrogenic potential when tested in in vivo or in vitro systems. 30 - 32 Local environment is an important factor for the differentiation of MSCs into chondrocytes or osteoblasts. 33 In the deep layer of defects, MSCs might have differentiated into osteoblasts and thus subchondral bone was well remodeled.…”

Cartilage Repair Using Composites of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Hyaluronic Acid Hydrogel in a Minipig Model

“…Among the various stem cell sources available, human umbilical cord blood‐derived MSCs (hUCB‐MSCs) have gained increasing attention, because they are easy to obtain and store. They also do not require a close human leukocyte antigen (HLA) match owing to the naïve nature of a newborn's immune system [5, 6].…”

Avaliação do comportamento de células indiferenciadas do cordão umbilical humano cultivadas sobre uma superfície de titânio nanotexturizado

“…DISCUSSÃOMuitos estudos mostram que as pesquisas envolvendo células-tronco mesenquimais vem despertando interesse na odontologia e medicina, trazendo avanços na área de implantodontia e reabilitação oral. Portanto, a utilização de células mesenquimais do cordão umbilical pode ter implicações terapêuticas, devido à sua facilidade de obtenção e armazenamento(Ende, 1999), além de apresentarem-se em grande quantidade e potencial proliferativo (Petsa e cols., 2009). O potencial de diferenciação celular é variado, podendo ser induzidas a se diferenciarem em adipócitos, células ósseas, musculares, cartilaginosas, endoteliais ou neuronais ( Weiss e cols., 2008; Fan e cols., 2011).…”