Effect of IGF-1 and Uncultured Autologous Bone-Marrow-Derived Mononuclear Cells on Repair of Osteochondral Defect in Rabbits

Abstract

Objective:To investigate the utility of bone-marrow-derived mononuclear cells (BMNCs) and insulin like growth factor-1 (IGF-1) in articular cartilage repair.Design:An osteochondral defect of 3 mm diameter and 5 mm depth was created in patellar groove of the left knee joint in each of 36 New Zealand White rabbits. The defect was filled with RPMI-1640 medium in group A (control), autologous BMNCs in group B, and autologous BMNCs plus IGF-1 in group C (n = 12). Healing of the defect was assessed by gross, scanning electron microscopic, radiographic, and histological examinations up to 90 days.Results:Gross and scanning electron microscopic examination of the healing site revealed superior gross morphology and surface architecture of the healing tissue in the animals of group C as compared to other groups. Radiographically on day 90, the defect area was not distinguishable from the surrounding area in group C, but a small circular defect area was still evident in groups A and B. The regenerated tissue was mostly hyaline in group C and fibrocartilage in groups A and B. The cells were well organized and showed better deposition of proteoglycans in groups C and B than in group A.Conclusions:It was concluded that implantation of bone-marrow-derived nucleated cells may facilitate the healing of osteochondral defects; however, the combination of BMNCs and IGF-1 induces faster and histologically better healing than the BMNCs alone.

The innate immune response to lower respiratory tract E. Coli infection and the role of the CCL2-CCR2 axis in neonatal mice

“…They noted improved healing of the bone fracture with early administration of recombinant human TNF, whereas inhibition of CCR2 signaling impaired bone healing. Bone marrow-derived mononuclear cells have also been shown to promote cartilage and diabetic wound healing [41,42] and attenuate stenosis of tissue engineered tracheas [43]. Taken together, our data supports the hypothesis that an intact CCL2-CCR2 axis is required to optimize the dynamics and effectiveness of a regulated innate immune response that results in timely resolution of microbial challenge in neonatal lungs.…”

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

“…A falta de resposta ao IGF-1 dos condrócitos pode ser parcialmente atribuída a uma série de fatores, incluindo o aumento na quantidade de proteínas de ligação do IGF-1 no espaço articular, falta de nutrição do tecido enxertado, micromovimentação do enxerto ou a dose administrada (Singh et al 2007). Além disso, em nenhum estudo com enxertos cartilaginosos foi obtido sucesso pleno na formação de um tecido com as características idênticas da cartilagem hialina normal (Kangarlu & Gahunia 2006, Heir et al 2012, Tiwary et al 2013.…”

“…A cartilagem articular possui uma capacidade limitada de reparação (Sellers et al 1997, Martin-Hernandez et al 2010, Dhollander et al 2011, Khan et al 2013, Kim et al 2013, Liu et al 2015, Jing et al 2015, devido ao ambiente avascular, ausência de inervação (Thiede et al 2012, Mollon et al 2013, pouca mobilidade dos condrócitos e capacidade reduzida de proliferação dos condrócitos maduros (Nam et al 2004, Chiang & Jiang 2009, o que impossibilita a recuperação do tecido cartilaginoso para seu estado original e geralmente resulta no preenchimento do defeito com tecido fibrocartilaginoso (Simank et al 2004, Tiwary et al 2013.…”

Animal mesenchymal stem cell research in cartilage regenerative medicine – a review

“…These defects are analogous to fissures or clefts seen in the early stages of OA (Hunziker 1999). Although the full-thickness defects heal spontaneously, they result in a mechanically and structurally weakened fibrous tissue that lacks integration with the native cartilage (Hunziker 1999;Arican et al 2006;Tiwary et al 2014).…”