The fabrication of an ICA-SF/PLCL nanofibrous membrane by coaxial electrospinning and its effect on bone regeneration in vitro and in vivo

Abstract

GBR is currently accepted as one of the most effective approaches for bone defect regeneration relating to dental implant. Icariin is the main active ingredient in the extraction of total flavonoids from the Chinese traditional herb Epimediumbrevicornum Maxim. In this study, ICA was successfully incorporated into the nanofibers barrier membrane (ICA-SF/PLCL) as osteoinduction factor by coaxial electrospinning and was released in a sustained and controlled manner. The entire release period included two stages: an initial burst stage (47.54 ± 0.06% on 5 d) and a decreasing and constant stage (82.09 ± 1.86% on 30 d). The membrane has good biocompatibility with BMMSCs anchored and significantly promoted its osteogenic activity. Moreover, in vivo experiment, bone defect covered by ICA-SF/PLCL membrane in rat cranium were statistically repaired compare to other groups. 12 weeks after implantation, in the test group, the new bone formation spread to cover most of the defect region with volume and density of approximately 15.95 ± 3.58 mm3 and 14.02 ± 0.93%. These results demonstrated that ICA-SF/PLCL nanofibrous membrane could be a promising barrier applicated for GBR.

Fabrication of tri-layered electrospun polycaprolactone mats with improved sustained drug release profile

“…In vitro cytotoxic study revealed that all groups in the study were not cytotoxic, and the cells grew as layers on the mat for 5 days as shown by SEM images. Notably, the reported tri-layered mats with highly sustained drug release profile are easy and straight forward to fabricate when compared to other methods such as coaxial / triaxial / microfluidic methods 3,17,[19][20][21][55][56][57][58] , hence can serve as a potential drug delivery vehicle in clinical applications. In summary, 12% PCLSW-2 with controlled initial burst and sustained release for 62 days, compared to all other groups in evaluation, seems to be best suited for delivery of IBU.…”

“…Various studies have reported the merits of coaxial/tri-axial fabrication of nanofibrous for sustained release of drugs 3,[19][20][21] . Although such methods yield efficient nanofiberous systems for controlled drug release, the complexity of fabrication process is a drawback.…”

Icariin-Functionalized Coating on TiO2 Nanotubes Surface to Improve Osteoblast Activity In Vitro and Osteogenesis Ability In Vivo

“…That said, icariin has a number of shortcomings, such as the first-pass effect after oral administration and low bioavailability, which limit its clinical application [20]. Previous studies have shown that icariin could be combined with small intestine submucosa scaffold to enhance bone regeneration [21]. Icariin was also incorporated into bioactive glass based scaffolds coated with gelatin for the sustained release of icariin [22].…”

Icariin-Loaded TiO2 Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells

“…ICA may exert favorable osseointegration effects by enhancing the differentiation of MSCs into osteoblasts while inhibiting the adipogenesis of bone marrow cells [17] and can treat osteoporosis by increasing bone mineral content [18]. Recently, ICA has been widely applied for bone tissue engineering due to its safe, nontoxic, and osteoinductive properties as well as its low cost [19,20]. The porous PLGA/TCP composite scaffold loaded with ICA was filled in mouse calvarial bone defects and induced significant new bone formation [21].…”