The expression pattern of hsp70 plays a critical role in thermal tolerance of marine demersal fish: Multilevel responses of Paralichthys olivaceus and its hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic and acute heat stress

Liu, Yifan; Ma, Donghui; Zhao, Chunyan; Xiao, Zhizhong; Xu, Shihong; Xiao, Yongshuang; Wang, Yanfeng; Liu, Qinghua; Li, Jun

doi:10.1016/j.marenvres.2017.06.015

Cited by 33 publications

(21 citation statements)

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“…Giving doses of MOE, Vit C, and NaHCO 3 to HS rabbits reduced HSP70 expression on both levels indicating the sensitivity of the latter protein to attenuated cellular stress. It indicated also the great role of HSP70 in thermal tolerance (Liu et al 2017). M. oleifera gold nanoparticles were found to Fig.…”

Section: Discussionmentioning

confidence: 95%

Influence of Moringa oleifera extract, vitamin C, and sodium bicarbonate on heat stress-induced HSP70 expression and cellular immune response in rabbits

Abdel‐Latif

Sakran

Badawi

et al. 2018

Cell Stress and Chaperones

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The current study aimed to test the effect of Moringa oleifera extract (MOE), vitamin (Vit) C, and sodium bicarbonate (NaHCO) on heat stress (HS)-induced alterations in rabbits. Five groups of rabbits were designed as control, HS, HS + MOE, HS + Vit C, and HS + NaHCO. HS groups were exposed to high temperatures, while treatments were given in drinking water for 6 weeks. Levels of blood cortisol, leptin, IFN-γ, TNF-α, and IL-10 were assayed using ELISA, while adrenaline was assayed calorimetrically. Expression of HSP70, FOXP3, T cell receptor (TCR) γ, and δ mRNA was tested using real-time (RT)-PCR, while HSP70 protein expression was tested using western blotting in liver and kidney tissues. Infiltration of regulatory T cells (Treg; CD25) and NK (CD56) cells were tested using immunohistochemistry (IHC). The levels of liver enzymes (ALT & AST), urea, and creatinine were assayed calorimetrically, while body weight gain (BWG) and feed conversion ratio (FCR) were calculated. The results showed increased levels of cortisol, adrenaline, leptin, IFN-γ, TNF-α, ALT, AST, urea, and creatinine but decreased IL-10 in the HS group. Increased expression of HSP70 on both mRNA and protein levels was associated with increased NK and γδ T cells versus decreased Treg cell infiltration in liver and kidney tissues of the HS group. In the same group, BWG was decreased, while FCR was increased with respect to the control group. All treatments used in this study reversed the effects of HS significantly. In conclusion, MOE, Vit C, and NaHCO can be added to rabbit diets for the amelioration of HS-induced symptoms.

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Section: Discussionmentioning

confidence: 95%

Influence of Moringa oleifera extract, vitamin C, and sodium bicarbonate on heat stress-induced HSP70 expression and cellular immune response in rabbits

Abdel‐Latif

Sakran

Badawi

et al. 2018

Cell Stress and Chaperones

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“…These proteins were first reported by Tissieres et al (1974) who by exposing larvae of Drosophila melanogaster to extreme temperature, observed different proteins in the salivary glands that were not present before the heat treatment. The Hsp's can be synthesized by most of the studied species, such as mammals (Basiricò et al, 2011;Li et al, 2011), insects (Zizzari & Ellers, 2011;Calabria et al, 2012), fish (Hofmann et al, 2000;Narum & Campbell, 2010;Dalvi et al, 2012;Liu et al, 2017), mollusks (Jackson et al, 2011;Zerebecki & Sorte, 2011;Lim et al, 2012;Thompson et al, 2012;Brokordt et al, 2015) echinoderms (Dong et al, 2010) and crustaceans (Ravaux et al, 2007). In all these species groups, the heat-shock response is highly conserved, as is the sequence of the genes that control the mechanism (Kawabe & Yokoyama, 2011;Park et al, 2012;Wojstasik & Wisnik, 2012).…”

Section: Heat-shock Proteins: Features and General Aspectsmentioning

confidence: 99%

“…Also, Farcy et al (2009) reported variable expression levels of Hsp70 in Crassostrea gigas, along different seasons, however, they found no correlation between the intensity of the expression of this gene and magnitude of changes in surface temperature of the sea. In this sense, Liu et al (2017) are concerned about ocean warming, resulting from global climate change, and its relationship with the ability to express Hsp70 and the thermal tolerance of demersal fish. On the other hand, Valenzuela-Castillo et al (2015) proved that in cultures of C. gigas from areas with large seasonal temperature fluctuations and extreme tidal range, as in the middle and upper Gulf of California, the difference between seasonal expression levels of Hsp70 is great.…”

Section: The Hsp70 Synthesis In Response To Heat Stressmentioning

confidence: 99%

Hsp70 function and polymorphism, its implications for mollusk aquaculture: a review

Valenzuela-Castillo

Sánchez‐Paz²,

Castro‐Longoria

et al. 2019

LAJAR

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Hsp70 proteins are widely found through several species, and they are considered among the most conservative proteins in nature, in both their sequence and function. They play an important role in the response mechanism to several different kinds of stress such as temperature and pathogens among others. In some species, the variations in the sequence of the Hsp70 gene result in intra-specific differential expression, and therefore in differences in thermo-tolerance of individuals as well as other productive characteristics. The study of the potential impact of the Hsp70 gene polymorphisms represents a field of study that could greatly help the mollusk farming industry.

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“…Moreover, the adaptive responses after heat stress also play a crucial role in recovery [5,6]. According to Liu et al [7], thermal-tolerant fish exhibit a shorter duration of heat stress response and earlier decline of HSP70 proteins when undergoing heat stress.…”

Section: Introductionmentioning

confidence: 99%

“…High temperatures cause cellular stresses and induce protein unfolding, which activates transcription factors, including heat shock factor 1 (HSF1), tumor protein p53 (p53), and nuclear factor-kappa B (NF-kB); such temperatures also allow HSF1 to produce HSPs (HSP70 and HSP90) in the cytoplasm [10,11]. HSPs are molecular chaperones involved in temperature tolerance [7,9], e.g., by preventing protein aggregation, assisting damaged proteins, and acting as primary genes, to cope with heat stress in the cell [12]. HSPs are associated with heat stress in tilapia [4], rainbow trout [9,13], killifish [14], and catfish [15], and heat stress induces tissue damage through apoptosis and necrosis, which increases the rate of cell proliferation and metabolism for maintaining cell activities [5,12,14,16].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Yamame (Oncorhynchus masou) in Normal Conditions after Heat Stress

Kraitavin

Yoshitake

Igarashi

et al. 2019

Biology

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Understanding the mechanism of high-temperature tolerance in cold-freshwater fish is crucial for predicting how certain species will cope with global warming. In this study, we investigated temperature tolerance in masu salmon (Oncorhynchus masou, known in Japan as ‘yamame’), an important aquaculture species. By selective breeding, we developed a group of yamame (F2) with high-temperature tolerance. This group was subjected to a high-temperature tolerance test and divided into two groups: High-temperature tolerant (HT) and non-high-temperature tolerant (NT). RNA was extracted from the gill and adipose fin tissues of each group, and the mRNA expression profiles were analyzed using RNA sequencing. A total of 2893 differentially expressed genes (DEGs) from the gill and 836 from the adipose fin were identified by comparing the HT and NT groups. Functional analyses were then performed to identify associated gene ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The HT group showed a high expression of heat shock protein 70 (HSP70) gene and enriched gene expression in the extracellular matrix (ECM), cell junction, and adhesion pathways in gill tissues compared to the NT group. The HT group also exhibited highly expressed genes in glycolysis and showed lower expression of the genes in the p53 signaling pathway in adipose fin tissues. Taken together, the difference of expression of some genes in the normal condition may be responsible for the difference in heat tolerance between the HT and NT yamame in the heat stress condition.

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The expression pattern of hsp70 plays a critical role in thermal tolerance of marine demersal fish: Multilevel responses of Paralichthys olivaceus and its hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic and acute heat stress

Cited by 33 publications

References 84 publications

Influence of Moringa oleifera extract, vitamin C, and sodium bicarbonate on heat stress-induced HSP70 expression and cellular immune response in rabbits

Influence of Moringa oleifera extract, vitamin C, and sodium bicarbonate on heat stress-induced HSP70 expression and cellular immune response in rabbits

Hsp70 function and polymorphism, its implications for mollusk aquaculture: a review

Transcriptome Analysis of Yamame (Oncorhynchus masou) in Normal Conditions after Heat Stress

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