Transcriptome Analysis of Yamame (Oncorhynchus masou) in Normal Conditions after Heat Stress

Abstract

Understanding the mechanism of high-temperature tolerance in cold-freshwater fish is crucial for predicting how certain species will cope with global warming. In this study, we investigated temperature tolerance in masu salmon (Oncorhynchus masou, known in Japan as ‘yamame’), an important aquaculture species. By selective breeding, we developed a group of yamame (F2) with high-temperature tolerance. This group was subjected to a high-temperature tolerance test and divided into two groups: High-temperature tolerant (HT) and non-high-temperature tolerant (NT). RNA was extracted from the gill and adipose fin tissues of each group, and the mRNA expression profiles were analyzed using RNA sequencing. A total of 2893 differentially expressed genes (DEGs) from the gill and 836 from the adipose fin were identified by comparing the HT and NT groups. Functional analyses were then performed to identify associated gene ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The HT group showed a high expression of heat shock protein 70 (HSP70) gene and enriched gene expression in the extracellular matrix (ECM), cell junction, and adhesion pathways in gill tissues compared to the NT group. The HT group also exhibited highly expressed genes in glycolysis and showed lower expression of the genes in the p53 signaling pathway in adipose fin tissues. Taken together, the difference of expression of some genes in the normal condition may be responsible for the difference in heat tolerance between the HT and NT yamame in the heat stress condition.

Comparative Transcriptomic Analysis of Largemouth Bass (Micropterus salmoides) Livers Reveals Response Mechanisms to High Temperatures

“…These results suggest that the 'ECM-receptor interaction' pathway in the livers of largemouth bass has a crucial role in the response of M. salmoides to HS. This finding is consistent with previous studies showing that the 'ECM-receptor interaction' pathway can be strongly induced in fish in response to HS [59,66,67].…”

Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

“…Therefore, those transport temperatures were recommended for further experiments. HSP is a protein that will respond to external stressful conditions [44]. It protects cells from extreme physiological, pathological, and environmental conditions, and plays a role in protein misfolding correction, preserving immature polypeptides from aggregation under stress [45,46].…”

Cardiac hemodynamics and ventricular stiffness of sea-run cherry salmon (Oncorhynchus masou masou) differ critically from those of landlocked masu salmon

“…Salmon have undergone four genome duplication events during their speciation, and consequently, their genomic DNA sequences are extremely complex and difficult to analyze [107][108][109]; however, recently, the complete genome of the Atlantic salmon was sequenced [79]. O. masou is also assumed to have a complex genome, but its genomic DNA sequences have been gradually deciphered, and comprehensive gene expression analysis is being attempted [110].…”