Influence of Moringa oleifera extract, vitamin C, and sodium bicarbonate on heat stress-induced HSP70 expression and cellular immune response in rabbits

Abstract

The current study aimed to test the effect of Moringa oleifera extract (MOE), vitamin (Vit) C, and sodium bicarbonate (NaHCO) on heat stress (HS)-induced alterations in rabbits. Five groups of rabbits were designed as control, HS, HS + MOE, HS + Vit C, and HS + NaHCO. HS groups were exposed to high temperatures, while treatments were given in drinking water for 6 weeks. Levels of blood cortisol, leptin, IFN-γ, TNF-α, and IL-10 were assayed using ELISA, while adrenaline was assayed calorimetrically. Expression of HSP70, FOXP3, T cell receptor (TCR) γ, and δ mRNA was tested using real-time (RT)-PCR, while HSP70 protein expression was tested using western blotting in liver and kidney tissues. Infiltration of regulatory T cells (Treg; CD25) and NK (CD56) cells were tested using immunohistochemistry (IHC). The levels of liver enzymes (ALT & AST), urea, and creatinine were assayed calorimetrically, while body weight gain (BWG) and feed conversion ratio (FCR) were calculated. The results showed increased levels of cortisol, adrenaline, leptin, IFN-γ, TNF-α, ALT, AST, urea, and creatinine but decreased IL-10 in the HS group. Increased expression of HSP70 on both mRNA and protein levels was associated with increased NK and γδ T cells versus decreased Treg cell infiltration in liver and kidney tissues of the HS group. In the same group, BWG was decreased, while FCR was increased with respect to the control group. All treatments used in this study reversed the effects of HS significantly. In conclusion, MOE, Vit C, and NaHCO can be added to rabbit diets for the amelioration of HS-induced symptoms.

Characterization of an inducible HSP70 gene in Chilo suppressalis and expression in response to environmental and biological stress

“…The HSP70 family is ubiquitous in living organisms (Feder and Hofmann 1999;Sørensen et al 2003) and functions as a molecular chaperone and antiapoptotic agent (Johnston et al 1998;Feder and Hofmann 1999;Yocum 2001;Hellmann 2001;McMillan et al 2005;Sørensen et al 2005;Koštál and Borovánsk 2009). Furthermore, HSP70 repressed the immune response in rabbits due to activation of IL-10-producing T cells (Abdel-Latif et al 2018). Interestingly, the activation of tumor cell death by heat treatment was dependent on hsp70 expression and production of γ δ T cells (Wei et al 1996).…”

Effect of Moringa oleifera leaf powder on skin mucosal immune responses and growth performance of guppy, Poecilia reticulata (Peter, 1860)

“…The M. oleifera contains ten essential amino acids, vitamins A, B complex, C, E and Carotenoids (Foidl, Makkar, & Becker, 2001), although the leaves are widely recognized as a food source for humans and as a dry season feed for animals (Becker & Makkar, 1999). Recently, moringa has been reported to reduce stress biomarker levels in different animals (Abdel‐Latif, Sakran, Badawi, & Abdel‐Hady, 2018).…”

Coenzyme Q10 attenuates rat hepatocarcinogenesis via the reduction of CD59 expression and phospholipase D activity

“…Samples were collected as previously described . Blood samples were collected and allowed to coagulate for serum preparation by centrifugation at 3500 × g for 15 minutes and then transferred into tubes and stored at −20°C.…”

“…The method was carried out as previously described. The total proteins were extracted from liver tissue using radioimmunoprecipitation buffer containing a protease inhibitor cocktail (Sigma). The protein quantity was performed using Bicinchoninic acid kit (Sigma).…”