Effect of
            <i>Moringa oleifera</i>
            leaf powder on skin mucosal immune responses and growth performance of guppy,
            <i>Poecilia reticulata</i>
            (Peter, 1860)

Bisht, Mayank; Kumar, Avdhesh; Shah, Tarang Kumar

doi:10.1111/are.14834

Cited by 12 publications

(9 citation statements)

References 21 publications

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“…This increasing response is likely correlated with the antibacterial properties of M. oleifera due to its high content of several immune-stimulant bioactive components like carotenoids, moringinine, vitamins, minerals, sterols, alkaloids, flavonoids, caffeoylquinic acids, and phytoestrogens ( 41 , 46 ). Our results agreed with ( 22 , 46 , 47 ) findings that reported increasing lysozyme activities with increasing moringa levels in the diet.…”

Section: Discussionsupporting

confidence: 93%

Moringa oleifera Leaf Powder Dietary Inclusion Differentially Modulates the Antioxidant, Inflammatory, and Histopathological Responses of Normal and Aeromonas hydrophila-Infected Mono-Sex Nile Tilapia (Oreochromis niloticus)

et al. 2022

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This study aimed to detect the impact of Moringa oleifera leaf powder dietary inclusion on the antioxidant and innate immune responses of mono-sex Nile tilapia fingerlings. A total of 180 fingerlings were allocated in a random method into three groups with triplicate each. One group (1st group) received the control diet (basal diet (BD) free of moringa) and the other groups (2nd and 3rd) fed BD containing M. oleifera leaf powder at 5 and 10% of the diet, respectively. After 6 weeks of feeding, fish were randomly redistributed into four replicates and rested for 24 h. Then, each fish in the first two replicates was injected with 0.2 mL of PBS, while the others were injected with 0.2 mL of A. hydrophila suspension (1.8 × 106 CFU/mL). Healthy fish fed on M. oleifera leaf powder showed enhanced immune response manifested by significant increases in phagocytic and lysozyme activities with a marked H/L ratio (P < 0.05). In addition, significant alterations of the lymphocytic and heterophilic population in circulation with increasing infiltration in tissue such as the spleen were noticed. Also, M. oleifera significantly upregulated the antioxidants, CAT and GPx, proinflammatory cytokines, IL1-β, IL-8, and IFN-γ relative mRNA levels. On the other hand, following A. hydrophila challenging conditions, M. oleifera caused downregulations of IL1-β, IL-8, and IFN-γ transcription levels, and also lowered the CAT and GPx mRNA levels. In addition, a marked reduction of leukocytic infiltration plus a significant improvement of the degenerative changes in intestinal architecture has occurred. So, M. oleifera leaf powder can be included in the fish diet to enhance immune response under normal health conditions and lower the infection-associated inflammatory response.

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Section: Discussionsupporting

confidence: 93%

Moringa oleifera Leaf Powder Dietary Inclusion Differentially Modulates the Antioxidant, Inflammatory, and Histopathological Responses of Normal and Aeromonas hydrophila-Infected Mono-Sex Nile Tilapia (Oreochromis niloticus)

et al. 2022

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“…MO has been demonstrated to enhance the growth performance of common carp (Cyprinus carpio) [35] and O. niloticus [28], and FMO has been reported to increase the growth performance of C. gibelio juveniles [9]. In this study, supplementation with MO and FMO had no negative effects on the growth performance of M. amblycephala juveniles, which was consistent with previous studies on guppy (Poecilia reticulata) [36] and O. niloticus [37]. These discrepancies may be attributed to differences in fish size and feeding conditions, as well as fish species [28].…”

Section: Discussionsupporting

confidence: 92%

The Application of Moringa oleifera Leaf Meal and Its Fermentation Products in the Diet of Megalobrama amblycephala Juveniles

et al. 2023

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This study assessed the potential applicability of Moringa oleifera leaf meal (MO) and fermented Moringa oleifera leaf meal (FMO) as feed supplements for aquatic animals. Five experimental diets, including the basal diet (control), 2.2% and 4.4% MO-supplemented diets (MO2 and MO4), and 2.2% and 4.4% FMO-supplemented diets (FMO2 and FMO4), were prepared for feeding Megalobrama amblycephala juveniles. After the eight-week feeding trial, the growth performance, muscle nutritional composition, plasma and hepatic biochemistry indicators were measured. The results demonstrated that MO and FMO had no detrimental effects on the growth performance of M. amblycephala juveniles. The muscle crude protein, crude lipid, and total free amino acids contents were significantly enhanced in the FMO4 group (p < 0.05). The liver acetyl-CoA carboxylase α mRNA level was significantly increased and the lipoprotein lipase mRNA level was markedly reduced in the FMO4 group (p < 0.05). Therefore, the FMO4 group exhibited a significant increase in plasma low-density lipoprotein cholesterol and triglyceride levels (p < 0.05). Compared to the control group, total superoxide dismutase and catalase activities were significantly increased in the FMO4 group (p < 0.05). The FMO2 and FMO4 groups exhibited an anti-inflammatory response by inhibiting the expression levels of toll-like receptor 4, nuclear factor-kappa B, and tumor protein P53 mRNA (p < 0.05). In conclusion, the 4.4% FMO treatment increased muscle crude protein content, enhanced lipogenesis, and improved the hepatic antioxidant abilities of M. amblycephala juveniles, while 2.2% FMO and 4.4% FMO improved the liver anti-inflammatory capacities.

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“…Moreover, Abd El‐Gawad et al (2020) illuminated that supplementing the powder of M. oleifera in diets also increased LYZ activities and IgM values of Nile tilapia. Furthermore, it was found that M. oleifera leaf powder significantly increased LYZ activities and ACH50 in skin mucous and plasma total immunoglobulins in treated guppies (Bisht et al, 2020). The enhancement of the serum immunological assays of Nile tilapia fed on MOL‐enriched diets may be correlated with several factors.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, dietary M. oleifera leaf extract can boost the immunological responses of gilthead seabream ( Sparus aurata ) (García‐Beltrán et al, 2020; Mansour et al, 2020), O. niloticus (Abd El‐Gawad et al, 2020; Monir et al, 2020) and also improve the survival rates of Mozambique tilapia ( Oreochromis mossambicus ) infected experimentally with Aeromonas hydrophila (Mbokane & Moyo, 2018). Furthermore, M. oleifera leaf powder can augment the skin mucosal immunity indices of guppies ( Poecilia reticulata ) (Bisht et al, 2020). Of interest, M. oleifera ‐based meals could strengthen the antioxidant status (Ahmed et al, 2020; Monir et al, 2020) and diminish the effects of physical stressors affecting fish (Elabd et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Effects ofMoringa oleifera‐based diets on growth, immunological responses, liver antioxidant biomarkers and expression of immune‐related genes in Nile tilapia (Oreochromis niloticus) raised in hapa‐in‐pond system

El‐Son

Hendam

Nofal³

et al. 2022

Aquaculture Research

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We have evaluated the impacts of supplementing diets with Moringa oleifera leaf extract (MOL) on growth, intestine histomorphometry, immunity, liver antioxidants and expression of immune genes in Nile tilapia raised in hapas mounted in earthen pond. Four MOL supplementation doses (0.0, 5.0, 7.0 and 9.0 g/kg) were used to make four experimental diets defined as reference (CONT), MOL5, MOL7 and MOL9 respectively. Fish (⁓61.5 g) were allocated in 4 triplicate groups and were fed the corresponding diets for consecutive 60 days. The highest final weight, weight gain, weight gain % and specific growth rate were found in the MOL5 group among all experimental groups. Feed conversion ratio was not changed by dietary MOL. When compared with the CONT group, there was a significant decline in MDA levels along with a significant rise in hepatic CAT, SOD and GPx enzymes in all MOL‐supplemented groups. Serum lysozyme activities, IgM levels and complement C3 were elevated in MOL groups than the CONT group. Moreover, dietary MOL preserved the normal histomorphology of the intestines of the treated Nile tilapia along with a significant improvement in some intestinal morphometric indices. There was significant increase in the mRNA expressions of inf‐γ, il‐10 and tnf‐α genes in the spleen tissue of MOL groups. However, the mRNA expression levels of il‐1β were significantly decreased in MOL groups. Of interest, the second‐order polynomial regression clarified that a 5.80 g MOL/kg diet is the optimum dietary supplementation level for better growth and no side effects on the health condition of fish. Altogether, dietary MOL could be recommended as a practical feed supplement to enhance growth, intestinal health, antioxidant capacity and immunity of Nile tilapia reared in hapas.

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Effect of Moringa oleifera leaf powder on skin mucosal immune responses and growth performance of guppy, Poecilia reticulata (Peter, 1860)

Cited by 12 publications

References 21 publications

Moringa oleifera Leaf Powder Dietary Inclusion Differentially Modulates the Antioxidant, Inflammatory, and Histopathological Responses of Normal and Aeromonas hydrophila-Infected Mono-Sex Nile Tilapia (Oreochromis niloticus)

Moringa oleifera Leaf Powder Dietary Inclusion Differentially Modulates the Antioxidant, Inflammatory, and Histopathological Responses of Normal and Aeromonas hydrophila-Infected Mono-Sex Nile Tilapia (Oreochromis niloticus)

The Application of Moringa oleifera Leaf Meal and Its Fermentation Products in the Diet of Megalobrama amblycephala Juveniles

Effects ofMoringa oleifera‐based diets on growth, immunological responses, liver antioxidant biomarkers and expression of immune‐related genes in Nile tilapia (Oreochromis niloticus) raised in hapa‐in‐pond system

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