The expression of the c‐<i>erb</i>B‐2 receptor protein in glandular salivary secretions

Streckfus, Charles F.; Bigler, Lenora; Dellinger, Tracy; Kuhn, Melinda; Chouinard, Normand; Dai, X.

doi:10.1111/j.1600-0714.2004.00255.x

Cited by 20 publications

(23 citation statements)

References 23 publications

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“…Determining the levels of erbB2 protein is a reliable method for early diagnosis and screening of cancer (Streckfus et al, 2004). Bayrak et al (2013) found that overexpression of genes is seen in many solid tumors, which is useful in disease prognosis and response to treatment.…”

Section: Discussionmentioning

confidence: 99%

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Salivary Levels of ErbB2 and CEA in Oral Squamous Cell Carcinoma Patients

Honarmand¹,

Farhad-Mollashahi²,

Nakhaee³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Oral squamous cell carcinoma (OSCC) is one of the ten most common causes of cancer death worldwide. Assessment of molecular changes can help detect and control lesions. The aim of this study was evaluation of salivary levels of ErbB2 and CEA in OSCC patients. In this case-control study, 27 OSCC patients and 26 healthy controls participated. After obtaining consent and filling out a questionnaire, unstimulated saliva samples were collected from people in the morning for measurement of the two markers using ELISA. Data were analyzed using Mann Whitney U test in SPSS 19 software with P<0.05 considered significant. Subjects were in the age range of 40 to 65 years. \Salivary level CEA was elevated in OSCC samples (42.6 ± 21.1 ng/ml) as compared to those of controls (22.6 ± 22.1ng/ml) (p< 0.01), but no significant variation was noted for ErbB2 (5.2 ± 1.8ng/ml and 4.93 ± 2.14ng/ml, p=0.28). The role of ErbB2 as a tumor marker in patients with OSCC must still be regarded as controversial and needs further studies to clarify any significance for early detection or screening. In contrast the salivary level of CEA may find application for early detection of patients.

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Section: Discussionmentioning

confidence: 99%

“…Measuring the level of erbB2 protein is a reliable method for the initial diagnosis and screening of cancers (Streckfus et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Salivary Levels of ErbB2 and CEA in Oral Squamous Cell Carcinoma Patients

Honarmand¹,

Farhad-Mollashahi²,

Nakhaee³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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“…It is also possible that RNAs are actively secreted. These RNAs could originate from secreting cells or they could be produced elsewhere in the body, travel through the circulatory system, and be secreted into the saliva (3,17,18 ). It has been reported that a breast cancer marker protein, c-erb-B-2, also known as HER-2/neu, is found in stratified saliva fractions (17 ).…”

Section: Discussionmentioning

confidence: 99%

“…Like many microand macromolecules, RNA in salivary gland secretions could originate from acinar cells or by circulation (1,3,17,18 ). GCF is also a likely source of RNA, because many blood cells and their cellular components are released into the oral cavity from GCF (19 ).…”

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confidence: 99%

Characterization of RNA in Saliva

et al. 2006

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Background:We have previously shown that human mRNAs are present in saliva and can be used as biomarkers of oral cancer. In this study, we analyzed the integrity, sources, and stability of salivary RNA. Methods: We measured the integrity of salivary RNA with reverse transcription followed by PCR (RT-PCR) or RT-quantitative PCR (RT-qPCR). To study RNA entry sites into the oral cavity, we used RT-PCR analysis of salivary RNA from the 3 major salivary glands, gingival crevice fluid, and desquamated oral epithelial cells. We measured stability of the salivary ␤-actin mRNA by RT-qPCR of salivary RNA incubated at room temperature for different periods of time. We measured RNA association with other macromolecules by filtering saliva through pores of different sizes before performing RT-qPCR. To assess RNA-macromolecule interaction, we incubated saliva with Triton X-100 for different periods of time before performing RT-qPCR. Results: In most cases, we detected partial-to fulllength salivary mRNAs and smaller amounts of middle and 3 gene amplicons compared with the 5. RNA was present in all oral fluids examined. Endogenous salivary ␤-actin mRNA degraded more slowly than exogenous ␤-actin mRNA, with half-lives of 12.2 and 0.4 min, respectively (P <0.001). Salivary RNA could not pass through 0.22 or 0.45 m pores. Incubation of saliva with Triton X-100 accelerated degradation of salivary RNA. Conclusions: Saliva harbors both full-length and partially degraded forms of mRNA. RNA enters the oral cavity from different sources, and association with macromolecules may protect salivary RNA from degradation.

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“…Saliva proteins may have diagnostic value for human oral and systemic diseases. For instance, the soluble fragments of the c-erbB-2 oncogene and CA 15-3 were found significantly up-regulated in the saliva samples of breast cancer patients than in those of healthy controls and patients with benign tumors [385,386]. Elevated levels of salivary CEA, defensin-1, TNF-alpha, IL-1, 6 and 8, and CD44 were detected in patients of oral cancer [387][388][389][390][391][392][393].…”

Section: Application To Human Disease Detectionmentioning

confidence: 99%

Human body fluid proteome analysis

2006

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The focus of this article is to review the recent advances in proteome analysis of human body fluids, including plasma/serum, urine, cerebrospinal fluid, saliva, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate fluid, tear fluid, and amniotic fluid, as well as its applications to human disease biomarker discovery. We aim to summarize the proteomics technologies currently used for global identification and quantification of body fluid proteins, and elaborate the putative biomarkers discovered for a variety of human diseases through human body fluid proteome (HBFP) analysis. Some critical concerns and perspectives in this emerging field are also discussed. With the advances made in proteomics technologies, the impact of HBFP analysis in the search for clinically relevant disease biomarkers would be realized in the future.

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The expression of the c‐erbB‐2 receptor protein in glandular salivary secretions

Cited by 20 publications

References 23 publications

Salivary Levels of ErbB2 and CEA in Oral Squamous Cell Carcinoma Patients

Salivary Levels of ErbB2 and CEA in Oral Squamous Cell Carcinoma Patients

Characterization of RNA in Saliva

Human body fluid proteome analysis

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