Human body fluid proteome analysis

Hu, Shen; Loo, Joseph A.; Wong, David T.

doi:10.1002/pmic.200600284

Cited by 483 publications

(411 citation statements)

References 456 publications

Supporting

Mentioning

406

Contrasting

Unclassified

Order By: Relevance

“…in Hu et al 1 ). It is an opinion paper aiming at provocative discussion on how proteomics is more or less suited to the discovery of new biomarkers, and how the power of proteomics can be used in improved strategies to maximize the probability of new markers discovery.…”

Section: Frame and Factsmentioning

confidence: 99%

How Shall We Use the Proteomics Toolbox for Biomarker Discovery?

2007

View full text Add to dashboard Cite

Biomarker discovery for clinical purposes is one of the major areas in which proteomics is used. However, despite considerable effort, the successes have been relatively scarce. In this perspective paper, we try to figure out and analyze the main causes for this limited success, and to suggest alternate strategies, which will avoid them, without eluding the foreseeable weak points of these strategies. Two major strategies are analyzed, namely the switch from body fluids to cell and tissues for the initial biomarker discovery step or, if body fluids must be analyzed, the implementation of highly selective protein selection strategies.Keywords : proteomics ; biomarkers ; plasma ; biological fluids ; cerebrospinal fluid ; Synopsis : this paper aims at stimulating discussions on how proteomics could be best used for

show abstract

Section: Frame and Factsmentioning

confidence: 99%

How Shall We Use the Proteomics Toolbox for Biomarker Discovery?

2007

View full text Add to dashboard Cite

show abstract

“…The protein composition of CSF is strictly regulated and can reflect the physiological or pathological state of the CNS (15). Thus in the present study, we addressed the challenge of staging HAT by analyzing CSF from T. b. gambiense patients using two complementary proteomic strategies: a classical approach based on two-dimensional gel electrophoresis , and quantitative mass spectrometry (MS) using isobaric tandem mass tag (TMT) technology (sixplex TMT ® MS/MS) (20).…”

mentioning

confidence: 99%

“…Proteomic approaches are increasingly being applied in biomedical research and clinical medicine to investigate body fluids as a source of biomarkers (15), including the diagnosis of neurological disorders such as Alzheimer's disease (16), Parkinson's disease (17), and multiple sclerosis (18,19). The protein composition of CSF is strictly regulated and can reflect the physiological or pathological state of the CNS (15).…”

mentioning

confidence: 99%

Discovery and Verification of Osteopontin and Beta-2-microglobulin as Promising Markers for Staging Human African Trypanosomiasis

Tiberti¹,

Hainard²,

Lejon

et al. 2010

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Human African trypanosomiasis, or sleeping sickness, is a parasitic disease endemic in sub-Saharan Africa, transmitted to humans through the bite of a tsetse fly. The first or hemolymphatic stage of the disease is associated with presence of parasites in the bloodstream, lymphatic system, and body tissues. If patients are left untreated, parasites cross the blood-brain barrier and invade the cerebrospinal fluid and the brain parenchyma, giving rise to the second or meningoencephalitic stage. Stage determination is a crucial step in guiding the choice of treatment, as drugs used for S2 are potentially dangerous. Current staging methods, based on counting white blood cells and demonstrating trypanosomes in cerebrospinal fluid, lack specificity and/or sensitivity. In the present study, we used several proteomic strategies to discover new markers with potential for staging human African trypanosomiasis. Cerebrospinal fluid (CSF) samples were collected from patients infected with Trypanosoma brucei gambiense in the Democratic Republic of Congo. The stage was determined following the guidelines of the national control program. The proteome of the samples was analyzed by two-dimensional gel electrophoresis (n ‫؍‬ 9), and by sixplex tandem mass tag (TMT) isobaric labeling (n ‫؍‬ 6) quantitative mass spectrometry. Overall, 73 proteins were overexpressed in patients presenting the second stage of the disease. Two of these, osteopontin and ␤-2-microglobulin, were confirmed to be potential markers for staging human African trypanosomiasis (HAT) by Western blot and ELISA. The two proteins significantly discriminated between S1 and S2 patients with high sensitivity (68% and 78%, respectively) for 100% specificity, and a combination of both improved the sensitivity to 91%. The levels of osteopontin and ␤-2-microglobulin in CSF of S2 patients (g/ml range), as well as the fold increased concentration in S2 compared with S1 (3.8 and 5.5 respectively) make the two markers good candidates for the development of a test for staging HAT patients. Molecular & Cellular Proteomics 9:2783-2795, 2010.

show abstract

“…Among the human body fluids, urine has gained widespread popularity as a protein source for disease biomarker discovery [1]. Not only is sample collection noninvasive, but urine also contains an abundance of proteins; recent investigations suggest the presence of well over 1500 proteins in urine [2].…”

Section: Introductionmentioning

confidence: 99%

A qualitative proteome investigation of the sediment portion of human urine: Implications in the biomarker discovery process

Mataija-Botelho¹,

Murphy²,

Pinto³

et al. 2009

Proteomics Clinical Apps

View full text Add to dashboard Cite

Inherent to the biomarker discovery process is a comparative analysis of physiological states. It is therefore critical that the proteome detection protocol does not bias the analysis. With urine, the sediment portion, obtained upon thawing frozen urine, is routinely discarded prior to proteome analysis. However, our results demonstrate that such a practice inadvertently induces bias, having significant implications in the biomarker discovery process. We present the first proteome investigation of human urinary sediments, identifying 60 proteins in this phase by MS. Many sediment proteins were also detected in the urinary supernatant, indicating that several proteins partition between the two phases. This partitioning is dependant on the pH of the sample, as well as the degree of sample agitation. As a consequence of discarding the sediment portion of urine, the concentration of potential candidate biomarkers in the supernatant phase will be altered or, in other instances, may be completely removed from the sample. To minimize this, the pH of all samples should first be normalized, and the samples vigorously vortexed prior to discarding the sediments. For more comprehensive biomarker investigations, we suggest that urinary sediments be analyzed along with the supernatant proteins.

show abstract

Human body fluid proteome analysis

Cited by 483 publications

References 456 publications

How Shall We Use the Proteomics Toolbox for Biomarker Discovery?

How Shall We Use the Proteomics Toolbox for Biomarker Discovery?

Discovery and Verification of Osteopontin and Beta-2-microglobulin as Promising Markers for Staging Human African Trypanosomiasis

A qualitative proteome investigation of the sediment portion of human urine: Implications in the biomarker discovery process

Contact Info

Product

Resources

About