The Expression Changes of Myelin and Lymphocyte Protein (MAL) Following Optic Nerve Crush in Adult Rats Retinal Ganglion Cells

Huang, Yongsheng; Xu, Yue; Cheng, Qiaochu; Yu, Shanshan; Gao, Yi; Shu, Qinmeng; Yang, Cheng; Sun, Yuan; Wang, Jiawei; Xu, Fan; Liang, Xiaoling

doi:10.1007/s12031-014-0332-5

Cited by 12 publications

(13 citation statements)

References 48 publications

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“…Thus, the activation of caspase-3 is involved in both the extrinsic and the intrinsic apoptotic pathway [30]. A growing body of evidence demonstrates that caspase-3 activation in RGCs is up-regulated after ON injury [39-41], and inhibition of caspase-3 activation increase the survival of RGCs [28, 37]. In our present study, the increased expression of cleaved-caspase-3 in NeuN-positive RGCs suggested a possible activation of caspase-3-dependent pathway after ONC.…”

Section: Discussionmentioning

confidence: 99%

“…Only left optic nerve of the rats was crushed as described in previous studies [39, 49] with slight modification. Briefly, after general anesthesia induced by 10 % chloral hydrate, the ON was exposed and isolated after the gently rotated downward in the orbit.…”

Section: Methodsmentioning

confidence: 99%

“…Immunofluorescent staining was performed on 8 μm thick frozen sections as our previously described [39, 52]. All retinal sections were prepared at 1-2 mm distance from the ONH, which was to ensure the use of equivalent fields for comparison.…”

Section: Methodsmentioning

confidence: 99%

“…Western blot analyses were performed as our previously described [39, 52]. 20-50 μg protein was separated with SDS-PAGE and transferred to PVDF membrane (Millipore, Bedford, MA).…”

Section: Methodsmentioning

confidence: 99%

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Wogonin prevents TLR4-NF-κB-medicated neuro-inflammation and improves retinal ganglion cells survival in retina after optic nerve crush

Yang

et al. 2016

Oncotarget

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Chronic neuro-inflammation is involved in the death of retinal ganglion cells (RGCs) in glaucoma. The aim of this study is to determine whether wogonin can suppress inflammatory responses and rescue RGCs death after optic nerve crush (ONC), an ideal animal model of glaucoma. Wogonin was administered intraperitoneally 10 min after establishment of ONC model. In this study, wogonin treatment reduced RGCs loss and inhibited RGCs apoptosis demonstrated by the increased Brn3a labeling RGCs at day 14 and the decreased cleaved caspase-3 expression at day 7 after ONC, respectively. In ONC model, number of GFAP-positive glial cells and iba1-positive microglial cells were increased, combined of the elevated level of pro-inflammatory cytokines released in retina at day 7. However, most of these responses were inhibited after wogonin treatment. The level of TLR4 expression, NF-κB-P65 nucleus location and NF-κB-P65 phosphorylation were increased in retina at day 1 after ONC, which was significantly reduced after wogonin treatment. These results demonstrated that wogonin protected RGCs survival and suppressed neuro-inflammation in retina after ONC by inhibiting TLR4-NF-κB pathways. We conclude that wogonin could be a possible strategy for the treatment of glaucoma.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Western blot analyses were performed as our previously described [39, 52]. 20-50 μg protein was separated with SDS-PAGE and transferred to PVDF membrane (Millipore, Bedford, MA).…”

Section: Methodsmentioning

confidence: 99%

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Wogonin prevents TLR4-NF-κB-medicated neuro-inflammation and improves retinal ganglion cells survival in retina after optic nerve crush

Yang

et al. 2016

Oncotarget

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“…They are found throughout the brain and spinal cord of the CNS and are one of the few mobile cell types in the CNS, which can proliferate readily at the sign of injury (Ransohoff and Brown, 2012). Oligodendrocytes are responsible for myelination of axons facilitating rapid conduction of the action potential from one node of Ranvier to the next (Huang et al, 2014). …”

Section: Use Of Cells In the In Vitro Modeling Of The Cnsmentioning

confidence: 99%

3D in vitro modeling of the central nervous system

Hopkins

DeSimone

Chwalek

et al. 2015

Progress in Neurobiology

196

188

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There are currently more than 600 diseases characterized as affecting the central nervous system (CNS) which inflict neural damage. Unfortunately, few of these conditions have effective treatments available. Although significant efforts have been put into developing new therapeutics, drugs which were promising in the developmental phase have high attrition rates in late stage clinical trials. These failures could be circumvented if current 2D in vitro and in vivo models were improved. 3D, tissue-engineered in vitro systems can address this need and enhance clinical translation through two approaches: (1) bottom-up, and (2) top-down (developmental/regenerative) strategies to reproduce the structure and function of human tissues. Critical challenges remain including biomaterials capable of matching the mechanical properties and extracellular matrix (ECM) composition of neural tissues, compartmentalized scaffolds that support heterogeneous tissue architectures reflective of brain organization and structure, and robust functional assays for in vitro tissue validation. The unique design parameters defined by the complex physiology of the CNS for construction and validation of 3D in vitro neural systems are reviewed here.

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Pim‐1 Expression in Rat Retina and its Changes after Optic Nerve Crush

Zhang

Wang

Huang

et al. 2018

The Anatomical Record

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Pim‐1 is a proto‐oncogene which has been discovered to involve in cell proliferation, differentiation, and survival. In this study, we observed the expression of Pim‐1 in neonatal and adult rat retina and the changes in rat retina following optic nerve crush (ONC) in order to explore the relationship between Pim‐1 and the survival of retinal ganglion cells (RGC). We discovered that Pim‐1 was distributed mainly in retinal pigment epithelial cells (RPE) and retinal ganglion cell layer (GCL) in normal newborn rats, and it appeared in RPE, cone rod cell layer and GCL in normal adult rats by immunohistochemistry. Our double immunofluorescent staining of Pim‐1 and γ‐synuclein further confirmed that Pim‐1 was localized in 80% of RGC. Moreover, we found that the amount of Pim‐1 mRNA and protein in adult rat retina was transiently increased after ONC and then decreased 2 weeks after ONC, and the expression level was lower than that of neonatal rat retina under all conditions. We also discovered that Pim‐1 expression in GCL detected by immunohistochemistry was upregulated at Day 1 and Day 3 after ONC, but downregulated at Day 14 after ONC when the survival of RGC was decreased and the apoptotic cells in GCL were increased by hematoxylin‐eosin staining, immunohistochemistry, and TUNEL detection. We suggest that the overexpression of Pim‐1 in the RGC is related to the optic nerve repair while the low expression of Pim‐1 in RGC may be associated with apoptosis and weak intrinsic regeneration ability of RGC. Anat Rec, 301:1968–1976, 2018. © 2018 Wiley Periodicals, Inc.

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The Expression Changes of Myelin and Lymphocyte Protein (MAL) Following Optic Nerve Crush in Adult Rats Retinal Ganglion Cells

Cited by 12 publications

References 48 publications

Wogonin prevents TLR4-NF-κB-medicated neuro-inflammation and improves retinal ganglion cells survival in retina after optic nerve crush

Wogonin prevents TLR4-NF-κB-medicated neuro-inflammation and improves retinal ganglion cells survival in retina after optic nerve crush

3D in vitro modeling of the central nervous system

Pim‐1 Expression in Rat Retina and its Changes after Optic Nerve Crush

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