The Evaluation of Hydroxyl Ions as a Nucleating Agent for Apatite on Electrospun Non-Woven Poly(<b><i>ϵ</i></b>-Caprolactone) Fabric

Kim, Hyung-Sup; Um, Seung‐Hoon; Rhee, Sang-Hoon

doi:10.1163/092050611x581507

Cited by 6 publications

(4 citation statements)

References 46 publications

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“…This resultantly upregulates apatite solubility and provokes the fast release of constituent elements of apatite such as calcium and hydroxyl ions in body fluid. This will enhance the hydroxyl carbonate apatite forming capacity51, 52 of anorganic bovine bone xenograft and resultant osteoconductivity.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a novel anorganic bovine bone xenograft with enhanced bioactivity and osteoconductivity

Cho

Kim

et al. 2013

J Biomed Mater Res

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A novel anorganic bovine bone xenograft with enhanced bioactivity and osteoconductivity was prepared by an ion substitution method using sodium hypochlorite. Bovine bone granules were defatted, washed, and then soaked in sodium hypochlorite solution at room temperature. Subsequently, the granules were dried and then heat-treated at 1000°C with sodium hypochlorite. As a control, bovine bone granules were prepared with the same conditions but without sodium hypochlorite treatment. Phase, functional group, and elemental analyses by XRD, FTIR, and EPMA showed that the granules heat-treated without and with sodium hypochlorite were pure hydroxyapatite and sodium-chlorine-bearing hydroxyapatite, respectively. After soaking in simulated body fluid (SBF) for 1 week, low crystalline hydroxyl carbonate apatite fully covered the surface of sodium-chlorine-bearing hydroxyapatite, whereas it formed little on the hydroxyapatite surface. After soaking in SBF and deionized water, ICP-AES and IC analyses showed that the dissolutions of calcium, sodium, chlorine, and hydroxyl ions from sodium-chlorine-bearing hydroxyapatite notably increased compared with those from hydroxyapatite. This resultantly increased the ionic activity product of apatite in SBF and induced new formation of low crystalline hydroxyl carbonate apatite. The cytotoxicity test by BCA assay showed that there were no statistically significant differences between hydroxyapatite and sodium-chlorine-bearing hydroxyapatite. In addition, sodium-chlorine-bearing hydroxyapatite showed better osteoconductivity in the calvarial defects of New Zealand white rabbits within 4 weeks compared with that of hydroxyapatite. The results suggest that this novel anorganic bovine bone xenograft possesses encouraging potential for use as a bone grafting material due to better bioactivity and osteoconductivity than hydroxyapatite.

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Section: Introductionmentioning

confidence: 99%

Preparation of a novel anorganic bovine bone xenograft with enhanced bioactivity and osteoconductivity

Cho

Kim

et al. 2013

J Biomed Mater Res

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“…To overcome these drawbacks when developing scaffold materials for bone tissue engineering, placing a simple apatite coating on a porous biodegradable polymeric scaffold seems to have a good chance of success because the surface is the only place on which biological reactions take place. Indeed, several methods have been reported for coating apatite crystals on the surface of polymers . However, these methods also have drawbacks such as spalling of apatite crystals during common handling procedures, for example, tailoring using scissors, folding, and holding with forceps.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, several methods have been reported for coating apatite crystals on the surface of polymers. [16][17][18][19][20][21][22][23][24] However, these methods also have drawbacks such as spalling of apatite crystals during common handling procedures, for example, tailoring using scissors, folding, and holding with forceps. Silanol groups [16][17][18][19][20] and silane coupling agents 21,22 have also been used to enhance apatite adhesion strength; these approaches yielded some success.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a non‐woven poly(ε‐caprolactone) fabric with partially embedded apatite surface for bone tissue engineering applications by partial surface melting of poly(ε‐caprolactone) fibers

Kim

Rhee

2017

J Biomedical Materials Res

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This article describes a novel method for the preparation of a biodegradable non-woven poly(ε-caprolactone) fabric with a partially embedded apatite surface designed for application as a scaffold material for bone tissue engineering. The non-woven poly(ε-caprolactone) fabric was generated by the electro-spinning technique and then apatite was coated in simulated body fluid after coating the PVA solution containing CaCl ·2H O. The apatite crystals were partially embedded or fully embedded into the thermoplastic poly(ε-caprolactone) fibers by controlling the degree of poly(ε-caprolactone) fiber surface melting in a convection oven. Identical apatite-coated poly(ε-caprolactone) fabric that did not undergo heat-treatment was used as a control. The features of the embedded apatite crystals were evaluated by FE-SEM, AFM, EDS, and XRD. The adhesion strengths of the coated apatite layers and the tensile strengths of the apatite coated fabrics with and without heat-treatment were assessed by the tape-test and a universal testing machine, respectively. The degree of water absorbance was assessed by adding a DMEM droplet onto the fabrics. Moreover, cell penetrability was assessed by seeding preosteoblastic MC3T3-E1 cells onto the fabrics and observing the degrees of cell penetration after 1 and 4 weeks by staining nuclei with DAPI. The non-woven poly(ε-caprolactone) fabric with a partially embedded apatite surface showed good water absorbance, cell penetrability, higher apatite adhesion strength, and higher tensile strength compared with the control fabric. These results show that the non-woven poly(ε-caprolactone) fabric with a partially embedded apatite surface is a potential candidate scaffold for bone tissue engineering due to its strong apatite adhesion strength and excellent cell penetrability. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1973-1983, 2017.

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“…Chloride substitution is expected to increase the system free energy of hydroxyapatite due to the ionic size differences between hydroxide and chloride, which would resultantly upregulate the solubility of chloride‐substituted hydroxyapatite. Increased solubility would speed up the release of constituent elements of apatite and positively influence its bioactivity and osteoconductivity.…”

Section: Introductionmentioning

confidence: 99%

Enhanced bioactivity and osteoconductivity of hydroxyapatite through chloride substitution

Cho

Yoo

Chung

et al. 2013

J Biomedical Materials Res

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The effect of chloride-substitution on bioactivity and osteoconductivity of hydroxyapatite (OHAp) was newly investigated. Chloride-substituted hydroxyapatites (ClAp) with low and high chloride concentrations were synthesized by reacting Ca(OH)2 and H3 PO4 with NH4 Cl of low and high concentrations, with subsequent sintering. As a control, pure OHAp was prepared under the same conditions but without addition of NH4 Cl. The ClAp showed markedly enhanced bioactivity in simulated body fluid (SBF) as the chloride substitution was increased. In contrast, OHAp did not show any bioactivity at all within the testing period. The solubility tests in deionized water also showed that the higher the chloride-substituting amount, the higher the dissolution amounts of the constituent elements of apatite, which directly affect bioactivity by increasing the degree of supersaturation of apatite in SBF. In addition, ClAp also showed noticeably higher osteoconductivity within the 4 weeks of implantation in calvarial defects of New Zealand white rabbits, compared with that of OHAp. The total system energy of the apatite calculated by the ab initio method showed that the higher the chloride-substituting amount, the higher the total system energy, which suggests that the ClAp was energetically less stable compared with OHAp. This result demonstrates the higher solubility of ClAp over that of OHAp in SBF and deionized water. The improved solubility of the OHAp enhances its bioactivity and consequent osteoconductivity. Taken together, it can be concluded that ClAp has encouraging potential for use as a bone grafting material due to its highly enhanced bioactivity and osteoconductivity compared with pure OHAp.

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The Evaluation of Hydroxyl Ions as a Nucleating Agent for Apatite on Electrospun Non-Woven Poly(ϵ-Caprolactone) Fabric

Cited by 6 publications

References 46 publications

Preparation of a novel anorganic bovine bone xenograft with enhanced bioactivity and osteoconductivity

Preparation of a novel anorganic bovine bone xenograft with enhanced bioactivity and osteoconductivity

Preparation of a non‐woven poly(ε‐caprolactone) fabric with partially embedded apatite surface for bone tissue engineering applications by partial surface melting of poly(ε‐caprolactone) fibers

Enhanced bioactivity and osteoconductivity of hydroxyapatite through chloride substitution

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