The estrogenic activity of certain phytoestrogens in the siberian sturgeon Acipenser baeri

Pelissero, C.; Bennetau, Bernard; Babin, Pierre; Menn, F. Le; Dunoguès, J.

doi:10.1016/0960-0760(91)90100-j

Cited by 151 publications

(79 citation statements)

References 32 publications

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“…However, according to the previous literature the estrogenic action of the isoflavones (including biochanin A, genistein, formonetin and daidzein) varies from one compound to another and from one study to another, suggesting inter-specific differences from one animal model to another [68] and/or technical differences in the systems used to assess the activities of these chemicals (in vivo tests [67]; in vitro tests [68]). However, it is important to note that when these compounds were tested in vivo in sturgeon, their activity did seem to be variable [27].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Vitellogenin synthesis in cultured hepatocytes; an in vitro test for the estrogenic potency of chemicals

Pelissero

Flouriot

Foucher

et al. 1993

The Journal of Steroid Biochemistry and Molecular Biology

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266

108

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Smnmary--We describe here an/n vitro technique to assess the estrogenic activity of chemicals. This technique is based on rainbow trout hepatocytes incubated in a basic medium free of any additional growth factors or estrogenic chemicals and uses the production of vitellogenin (VTG) as a marker for the estrogenic potency of the compounds tested. The system allows at least some of the metabolic transformations which are undertaken by the liver cells/n vivo and could therefore be used for xenobiotic compounds which exhibit estrogenic activities after liver metabolic transformation. A dose-response curve was always consistently obtained using estradiol-17fl (E2), with a mid point at around 100 nME 2 and a maximum response at around 1000nM. Established estrogens such as 17al ethynylestradiol (EE2) or diethylstilboestrol (DES) were also tested. EE 2 appeared to be equipotent with E2 and DES slightly less potent. E2 conjugates were, perhaps surprisingly, also very potent. Estradiol-3-sulfate was equipotent with E2 and estradiol-17fl-glucuronide approx. 10% as potent. Other steroids such as androgens and progesterone, though active in the bioassay, were 3 orders of magnitude less potent than E 2. Of the various steroids tested, only cortisol, at concentrations up to 50 #M, was completely inactive. Six different phytoestrogens were tested in the assay. All were weakly estrogenic, possessing approximately one thousanth the potency of E 2 (they were as potent as the androgens and progesterone). All six phytoestrogens, as well as the androgens and progesterone, were tested in the presence of tamoxifen. In all cases tamoxifen reduced the production of VTG significantly, demonstrating that the estrogenic action of all of these compounds was most likely mediated by the E 2 receptor. The potencies determined here may not reflect the situation/n vivo but can provide complementary results about the activity of chemicals which need an hepatic metabolization to be estrogenic. Hepatocyte cultures would profitably be developed in other species to sustain these results.

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Section: Resultsmentioning

confidence: 99%

“…The phytoestrogens tested were synthesized according to Pelissero et al [27]. The steroids tested were either USP standards or Sigma standards.…”

Section: Compounds Testedmentioning

confidence: 99%

Vitellogenin synthesis in cultured hepatocytes; an in vitro test for the estrogenic potency of chemicals

Pelissero

Flouriot

Foucher

et al. 1993

The Journal of Steroid Biochemistry and Molecular Biology

Self Cite

266

108

View full text Add to dashboard Cite

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“…Phytostrogens increased vitellogenin synthesis in Tilapia primary hepatocytes (Turker and Takemura, 2011). Furthermore, phytoestrogens are able to stimulate vitellogenin synthesis in vivo in Siberian sturgeon Acipenser baeri (Pelissero et al, 1991) and common carp, Cyprinus carpio, (Turker and Bozcaarmutlu, 2009), in vitro in rainbow trout, Oncorhynchus mykiss, hepatocyte (Pelissero et al, 1993) and in vivo / in vitro in Siberian sturgeon, Acipenser baeri, and rainbow trout (Latonnelle et al, 2002). Turker and Takemura (2011) in their study indicated that plant extract such as fennel, fenugreek, aniseed, safflower, flaxseed, liqurice, pomegranate and soybean were considerably less potent than estradiol.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Nazari¹,

Roozbehani²

2015

Turk. J. Fish. Aquat. Sci.

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Ornamental fish breeding and growing are an important sector of aquaculture, which has increased income and occupations in the country in recent years. Presenting the new methods and producing the new food supplements could have effects on efficiency and sustainable development in this new technology. In the present study, the effect of ethanolic extract of F. vulgar having phytoestrogenic compounds on growth performance and fertility rate and gonadal tissue changes of guppy was tested for offering it as a food supplement in the aquaculture. 360 juvenile guppies were distributed into 12 glass aquaria at 30 fish/ tank in triplicate. Four diets containing varying levels of alcoholic extract of F. vulgar seed at 0. 75, 100, 125 µl/g were prepared and fed to the fish twice daily at 3% of their body weight for 133 days. Biometry of the groups was done every 15days. The born fry were separated from the mothers and counted. One fish from all groups were fixed in formalin buffered 10% every 15days for histology. 5 microns sections were prepared and stained with H&E technique. The results for each group were compared statistically with those for the control. Analysis of the results showed a significant increase in weight gain and fertility rate in group T2 (100 µl/g). Gonad cells increased in the test groups comparing with control group simultaneously with raise in phytostrogenes in tests groups. In the follicles, enlarge granulose layer with increase in number of ovules in different evolution stage (O 1 -O 7 ), were seen in test groups. The increase in fertility rate in tests groups may be due to the increase in the level of estrogenic compound which in turn could be due to the action of the extract present in the diet. The observation made in the present investigation show that F. vulgar can act as a phytoestrogenic compound at low concentration and hence F. vulgar can be considered as a potential as well as a natural additive to fish feed in commercial aquaculture to augment the fertility rate.

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“…Pelissero et al (1991b) subsequently reported direct evidence for oestrogenic activity of daidzein, biochanin A, genistein, equol and coumestrol after intraperitoneal injection. However, the activity was considerably less compared with estradiol.…”

Section: Biological Effects In Fishmentioning

confidence: 99%