The enzyme-linked immunosorbent assay (ELISA) for determination of IgG and IgM antibodies after infection with mumps virus

Virus genotypes and responses of serum-specific antibodies in children with primary mumps and mumps reinfection

“…In this study, IgM antibody was negative in the acute phase in more than half of children with mumps reinfection that had developed after a natural infection. These findings suggest that previously reported patients diagnosed with primary mumps who showed a negative IgM antibody in the acute phase may have had inflammatory salivary gland diseases other than mumps (1) or mumps reinfection after natural infection (1)(2)(3)(4).…”

“…Previous reports showed that IgM antibodies were negative in some patients with primary mumps during the acute phase. The diagnoses of primary mumps in those reports were made based on physical findings (1), significant increases in serum complement fixation test titers (2), significant increases in IgG antibody titers (3,4), or positive virus cultures (2). In this study, IgM antibody was negative in the acute phase in more than half of children with mumps reinfection that had developed after a natural infection.…”

“…To make a definitive diagnosis of reinfection, viral cultures and detection of mumps-specific IgM and IgG antibodies in paired sera are required. Although there are some reports on the response of virus-specific serum antibodies in patients with primary mumps and in those with mumps reinfection (1)(2)(3)(4)(5)(6)(7), no studies have reported the detection of specific IgM and IgG antibodies in paired sera of children with mumps reinfection occurring after natural infection. In this study, we investigated responses of both antibodies in such cases as well as in previously vaccinated children with mumps and in those with primary mumps infection.…”

S-Sulfonation: a Reversible Chemical Modification of Human Immunoglobulins Permitting Intravenous Application

“…Quantitative determination of complement con sumption (anticomplementary activity, ACA) was per Antibody titers against cytomegalovirus, measles, mumps, rubella, and varicella zoster were measured using an ELISA technique [19,20]. Classical precipitin analysis was carried out as described by Eisen et al [21].…”

Rapid Serological Diagnosis of Mumops Virus Infection by an Enzyme-Linked Immunosorbent Assay of Mumps IgG and IgM Antibodies

“…Several reports described enzyme-linked immunosorbent assay (ELISA) as a sensitive and simple method for detecting mumps antibodies [ 1-51. The purposes of this paper are (1) to report a simple ELISA of mumps IgM antibody and of IgG antibody, (2) to evaluate the usefulness of this assay as a means of rapid diagnosis of mumps virus infection, and (3) to assure the validity of the ELISA of mumps IgM and IgG antibodies.…”