Virus genotypes and responses of serum-specific antibodies in children with primary mumps and mumps reinfection

Sakata, Rika; Nagita, Akira; Kidokoro, Minoru; Kato, Atsushi; Oginö, Kazuhíde

doi:10.1038/pr.2015.141

Cited by 9 publications

(10 citation statements)

References 9 publications

(10 reference statements)

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“…It is interesting to note that about 55.2% of healthy individuals are positive by FRNT (National Institute of Virology, 2014). Because of the unavailability of a mumps IgG avidity testing facility, recent or past mumps infection could not be determined in these individuals with suspected mumps, as described in previous studies (7,16). Altogether, 26.34% of specimens from individuals suspected clinically of having mumps showed neither MuV RNA nor MuV IgM antibodies; those individuals may therefore not have had true mumps.…”

Section: Discussionmentioning

confidence: 98%

Utility of neutralization test for laboratory diagnosis of suspected mumps

Vaidya

Hamde²,

Kumbhar

et al. 2018

Microbiology and Immunology

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Mumps is an infectious disease caused by mumps virus (MuV), which belongs to the family Paramyxoviridae and genus Rubulavirus. Typical symptoms of mumps include fever and swelling of the parotid glands; however, mumps can be asymptomatic. Mumps is diagnosed by molecular and serological methods (i.e., PCR and Enzyme Immunoassay [EIA]); however, both methods have pros and cons. This study was performed to compare the diagnostic utility of a focus reduction neutralization test (FRNT) to that of MuV-specific commercial IgM and IgG antibody EIA in patients suspected of having mumps. One hundred-eighty six samples collected during mumps outbreak in 2012-16 were studied. Samples (n = 80) were tested by all the three serological assays and showed 70.4%, 83% and 92.5% positivity by IgM EIA, IgG and FRNT, respectively. In all, 58.8% samples (n = 47) tested positive in all three assays. Concordance between mumps RT-PCR and IgM EIA was highest during the first 2-5 days and decreased with increasing time post-onset. Mumps FRNT results agreed with those of RT-PCR/IgM EIA from the second week onwards, whereas the results of mumps IgG EIA agreed with those of RT-PCR/IgM EIA from post-onset days 3-10. These findings suggest the utility of a FRNT for laboratory diagnosis of mumps in countries whose populations are not immunized against this infection.

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Section: Discussionmentioning

confidence: 98%

Utility of neutralization test for laboratory diagnosis of suspected mumps

Vaidya

Hamde²,

Kumbhar

et al. 2018

Microbiology and Immunology

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“…El estudio serológico de la parotiditis viral depende de si el paciente tiene una primoinfección, una reinfección luego de la infección natural o una infección postvacunación. En casos de primoinfección suele detectarse simultáneamente positividad para los anticuerpos IgM e IgG, situación encontrada en nuestros dos casos 15 . Para casos con reinfección se ha descrito una seropositividad IgM (en aproximadamente 60%) acompañada de un aumento de los valores IgG positivos basales durante el seguimiento.…”

Section: Discussionunclassified

“…Para casos con reinfección se ha descrito una seropositividad IgM (en aproximadamente 60%) acompañada de un aumento de los valores IgG positivos basales durante el seguimiento. Sin embargo, el estudio serológico en personas previamente vacunadas ha revelado una muy baja sensibilidad para los anticuerpos IgM (< 10%) [15][16][17] . Los antecedentes entregados sugieren que, en el contexto actual, es más útil el diagnóstico molecular que la caracterización serológica de los casos, enfoque aplicado en algunos centros universitarios y que, además, permite una tipificación viral 18 .…”

Section: Discussionunclassified

Meningitis aséptica en pacientes con parotiditis viral durante un brote epidémico

Fíca

Díaz

Aguilar

et al. 2019

Rev. chil. infectol.

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Aseptic meningitis associated to mumps during an epidemic outbreak We report two cases of acute aseptic meningitis associated to mumps in middle-aged women, one pregnant. Both presented shortly after parotid gland enlargement. Neurological complications were suspected by headache, fever and meningeal signs and confirmed by CSF findings (mononuclear predominant pleocytosis) with negative results for alternative causes. Mumps were confirmed by positive IgM and IgG serology. Both patients were discharged with a favorable evolution and complete disappearance of symptoms. Cases were concurrent with a regional mumps outbreak. Conclusions: Aseptic meningitis is a rare mumps-associated neurological complication. Its diagnostic can be achieved by precedent parotid enlargement, mononuclear pleocytosis in the CSF and positive IgM and IgG serology or viral detection by PCR in urine or salivary samples. This complication would be more probably observed during mumps outbreaks.

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“…29 The diagnoses of the aforementioned patients without antibody or CMI were made based on the presence of high IgG antibody and high avidity of IgG antibody without demonstrable IgM antibody, which has been used to determine reinfection. 9,13,14,23,[29][30][31] Furthermore, in order to ensure the accuracy of diagnosis of reinfection, virus isolation and/or detection of the mumps virus genome 12 was added. That report showed that the viral load in reinfection was lower than in primary infection, and that reinfection was not a rare event after natural infection.…”

Section: Discussionmentioning

confidence: 99%

“…Under this condition, breakthrough infection after vaccination or symptomatic reinfection after natural infection of mumps has been noted in Japan. 9,[11][12][13][14] Efficacy studies regarding mumps vaccines have noted rates as low as 64% after one dose, rising to 88% after two doses, [15][16][17] although high rates (>90%) of seroconversion of the IgG antibody have been reported after mumps vaccination. 7,18 Immunogenicity studies that measure only anti-mumps specific IgG overestimate vaccine efficacy.…”

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confidence: 99%